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Isikhungo se-R & D Center
Ukuqalisa
Ama-antibiotic yisigaba sama-metabolites asekondari akhiqizwa ama-microorganisms akhiqizwa ama-microorganisms (kufaka phakathi amagciwane, isikhunta, ama-actecomycetes) noma amakhompiyutha afanayo ahlanganisiwe ahlanganisiwe noma ahlanganisiwe. Ama-antibiotic angavimba ukukhula nokusinda kwamanye ama-microorganisms. I-anticiotic yokuqala etholwe ngumuntu, i-penicillin, yatholakala ngudokotela waseBrithani waseBrithani u-Alexander Fleming ngo-1928. Wabona ukuthi amagciwane aseduze kwesikhunta asikwazanga ukukhula esikhunjeni. Wathumela ukuthi isikhunta kufanele sithengele into ephilayo, eyayinguPhenicillin ngo-1928. Kodwa-ke, izithako ezisebenzayo azihlanzwa ngaleso sikhathi. Ngo-1939, u-Ernst Chain noHoward Florey wase-Oxford University banquma ukuthuthukisa isidakamizwa esingaphatha izifo ezingamagciwane. Ngemuva kokuxhumana noFleming ukuthola ama-perking, akhishwe ngempumelelo futhi ahlanza i-penicillin evela ezinhlamvwini. Ngokuthuthuka kwabo ngempumelelo kwe-penicillin njengomuthi wokwelapha, uFleming, uchungechunge noFlorey kwabelana ngomklomelo we-1945 Nobel kwezokwelapha.
Ama-antibiotic asetshenziswa njengama-antibacterial agents ukwelapha noma ukuvimba izifo zamagciwane. Kunemikhakha eminingana eminingana yama-antibiotics asetshenziswa njenge-antibacterial agents: β-lactam antibiotic (kufaka phakathi i-penicillin, i-antibootic, imithombo ye-antibiotic), njll. Imithombo yokwenziwa antibiotic), njll. Imithombo ye-antibiotic ifaka biological Fermentation, semi-synthesis kanye synthesis okuphelele. Ama-antibiotic akhiqizwa ukuvutshelwa kwemvelo kudinga ukuguqulwa ngezindlela zamakhemikhali ngenxa yokuqina kwamakhemikhali, imiphumela emibi enobuthi, imiphumela ye-antibacterial kanye nezinye izingqinamba. Ngemuva kokuguqulwa ngamakhemikhali, ama-antibiotic angathola ukuqina okukhuphukayo, anciphise imiphumela emibi enobuthi, anwetshiswe ama-antibacterial spectrum, ukuncishiswa kwemithi, futhi ngaleyo ndlela kuthuthukiswe i-bioavailability, bese wenza umphumela othuthukisiwe wokwelashwa kwezidakamizwa. Ngakho-ke, ama-antibiotic okwenziwa ngama-semi-zokwenziwa njengamanje eyinkomba ethandwa kakhulu ekuthuthukisweni kwezidakamizwa ezilwa namagciwane.
Ekuthuthukisweni kwama-anti-antibitic anokwenziwa, ama-antibiotics anezici zokuhlanzeka okuphansi, imikhiqizo eminingi ngemikhiqizo nezinto eziyinkimbinkimbi selokhu zithathwe yimikhiqizo yokuvutshelwa kwe-microbial. Kulokhu, ukuhlaziywa nokulawulwa kokungcola kuma-anti-antibiotics anokwenziwa kubaluleke kakhulu. Ukuze ukhombe ngempumelelo futhi ubonise ukungcola, kuyadingeka ukuthola inani elanele lokungcola kusuka kumkhiqizo wokwenziwa we-semi-zokwenziwa antibiotiotic. Phakathi kwamasu okulimazeka asetshenziswa ngokujwayelekile, i-flash chromatography iyindlela esebenza ngemali enezimali ezifana nenani elikhulu lesampula, izindleko eziphansi, isikhathi sokugcina, njll. I-Flash Chromatography ibilokhu iqashwe kakhulu ngabacwaningi besayensi.
Kulokhu okuthunyelwe, ukungcola okuyinhloko kwe-aminoglycoside antibinoside antibiotic kusetshenziswe njengesampula futhi kuhlanjululwe yi-Sepaflash C18AQ Cartridge kuhlanganiswe nomshini we-chromatography sepawean ™. Umhlangano womkhiqizo oqondiwe Izidingo zitholwe ngempumelelo, ziphakamisa isixazululo esisebenza kahle kakhulu sokuhlanzwa kwalezi zinhlanganisela.
Isigaba Sokuhlola
Isampula lanikezwa ngomusa inkampani yendawo yemithi. Isampula ibiwuhlobo lwama-modecyclic ama-carbohydrate kanye nesakhiwo salo samangqamuzana kwakufana ne-aminoglycoside antibiotic. I-polarity yesampula yayiphakeme kakhulu, okwenza kwanyanyeka kakhulu emanzini. Umdwebo we-schematic wesakhiwo samangqamuzana esampula saboniswa kuMfanekiso 1. Ubumsulwa besampula eluhlaza belingaba ngu-88% njengoba kuhlaziywe yi-HPLC. Ngokuhlanzwa kwalezi zinhlanganisela ze-polarity ephezulu, isampula lizogcinwa kalula kumakholomu ajwayelekile e-C18 ngokuya ngolwazi lwethu lwangaphambili. Ngakho-ke, ikholomu ye-C18AQ isetshenziselwe ukuhlanzwa kwesampula.
Umdwebo 1. Umdwebo we-schematic wesakhiwo samangqamuzana esibonelo.
Ukulungiselela isixazululo sesampula, isampula engu-50 mg uCrude sachithwa emanzini ahlanzekile ayi-5 ml bese ifakwa ngo-ultrasonion ukuze ikwenze kube yisixazululo esicacile ngokuphelele. Isixazululo sesampula sabe sesifakwa kwikholomu ye-flash ngumjovo. Ukusethwa kokuhlola kokuhlanzwa kwe-flash kwafakwa ohlwini etafuleni 1.
| Into yokwenza utho | Umshini weSepabean ™ Umshini 2 | |
| Ama-cartridges | I-12 g sepaflash C18AQ RP Flash Carthridge (spherical Silica, 20 - 45μm, Inombolo ye-Order: Inombolo ye-SW-5222-012-SP (AQ)) | |
| Umbuzi | 204 NM, 220 NM | |
| Isigaba | Solvent a: amanzi Solvent b: acetonitrile | |
| Izinga Lokugeleza | 15 ml / min | |
| Ukulayisha isampula | 50 mg | |
| I-gradient | Isikhathi (min) | Solvent b (%) |
| 0 | 0 | |
| 19.0 | 8 | |
| 47.0 | 80 | |
| 52.0 | 80 | |
Imiphumela nengxoxo
I-Flash Chromatogram yesampula ku-C18AQ cart cartridy yaboniswa kuMfanekiso 2. Njengoba kukhonjisiwe kuMfanekiso 2, isampula eliphakeme kakhulu le-polar lagcinwa ngempumelelo kwi-Cartidge Cartridge. Ngemuva kokunyathela kwezingxenyana eziqoqiwe, umkhiqizo oqondiwe unobumsulwa bama-96.2% (njengoba kukhonjisiwe kuMfanekiso 3) ngokuhlaziywa kwe-HPLC. Imiphumela iveze ukuthi umkhiqizo ohlanziwe ungasetshenziswa futhi esiteshini esilandelayo sokucwaninga nentuthuko.
Umdwebo 2. I-flash chromatograph yesampula ku-C18AQ cartridge.
Umdwebo 3. I-HPLC Chromatogram yomkhiqizo oqondiwe.
Ekuphetheni, iSepaflash C18AQ RP Flash Carthridge ihlanganiswe nesidlali se-Chromatography System Sepabean Syste Machine inganikeza isixazululo esisheshayo nesisebenzayo sokuhlanzwa kwamasampula anamakhaza.
Mayelana ne-Sepaflash C18AQ RP Flash Cartridges
Kunochungechunge lwama-cartridgeges we-sepaflash C18AQ RP Flash anokucaciswa okuhlukile kubuchwepheshe beSantai (njengoba kukhonjisiwe kuThebula 2).
| Inombolo yento | Usayizi wekholomu | Izinga Lokugeleza (ml / min) | Max.prestrure (PSI / Bar) |
| SW-5222-004-SP (AQ) | 5.4 g | 5-15 | 400 / 27.5 |
| SW-5222-012-SP (AQ) | 20 g | 10-25 | 400 / 27.5 |
| SW-5222-025-SP (AQ) | 33 g | 10-25 | 400 / 27.5 |
| SW-5222-040-SP (AQ) | 48 g | 15-30 | 400 / 27.5 |
| SW-5222-080-SP (AQ) | 105 g | 25-50 | 350 / 24.0 |
| SW-5222-120-SP (AQ) | 155 g | 30-60 | 300 / 20.7 |
| SW-5222-220-SP (AQ) | 300 g | 40-80 | 300 / 20.7 |
| SW-5222-330-SP (AQ) | 420 g | 40-80 | 250 / 17.2 |
Ithebula 2. Sepaflash C18AQ RP Flash Cartridges. Izinto zokupakisha: I-Spherical C18 esebenza kahle kakhulu
Ukuthola eminye imininingwane ngokuchazwa okunemininingwane yomshini we-Sepabean ™, noma imininingwane yoku-oda kuma-cartridge we-Sepaflash Series, sicela uvakashele iwebhusayithi yethu.
Isikhathi sePosi: Oct-26-2018