URui Huang, bo xu
Isikhungo se-R & D Center
Ukuqalisa
I-ION Exchange Chromatography (IEC) yindlela ye-Chromatographic ejwayelekile esetshenziselwa ukuhlukanisa nokuhlanza amakhompiyutha avezwa ngefomu le-ionic ngesixazululo. Ngokwezindawo ezihlukile zokushaja zama-ion ashintshiwe, i-IEC ingahlukaniswa ngezinhlobo ezimbili, i-coition ukushintshanisa i-chromatography kanye ne-anion exchange chromatography. Ku-cotion screen chromatography, amaqembu acidic ahlanganiswe ebusweni bemidiya yokuhlukanisa. Isibonelo, i-sulfonic acid (-SHOH) iqembu elisetshenziswa kakhulu ku-cotion exchange eqinile (i-SCX), elihlukanisa i-H + neqembu elikhokhisiwe leqembu--ke-ke-ke-ke i-adsorb ezinye izixazululo kwikhambi. E-Anion Exchange Chromatography, amaqembu e-alkaline ahlanganiswe ebusweni bemidiya yokuhlukanisa. Isibonelo, i-Quaternary Amine (-NR3OH, lapho r yi-R yi-hydrocarbon) imvamisa isetshenziswa ku-antion exchange eqinile (i-sax), ehlukanisa i-oh- neqembu elikhokhiswayo
Phakathi kwemikhiqizo yemvelo, ama-flavonoids adonsele ukunakwa kwabaphenyi ngenxa yeqhaza labo ekuvinjelweni nasekwelashweni kwezifo zenhliziyo. Njengoba ama-molecule e-flavonoid ane-acicic ngenxa yokuba khona kwamaqembu we-phenolic hydroxyl, i-ini Exchange Chromatography yindlela ehlukile ngaphezulu kwesigaba esijwayelekile noma i-chromatography yesigaba esibuyiselwe kulezi zinhlanganiso ze-acidic. Ku-Flash Chromatography, imithombo yokuhlukanisa evame ukusetshenziswa kwe-Ion Exchange yi-Silica Gel Matrix lapho amaqembu e-ION Exchange Amaqembu ahlanganiswe ebusweni bayo. Izindlela ezisetshenziswa kakhulu ze-ION Exchange Izinhlobo ku-Flash Chromatography kukhona i-SCX (imvamisa i-Sulfonic Acid Group) ne-SAX (imvamisa i-Amine Group). Encwadini eshicilelwe ngaphambili yesicelo esishicilelwe nesihloko esithi "Ukusetshenziswa kwe-SepaFlash eqinile ye-Contaction Crolography Collents ekuhlanzeni amakhompiyutha e-Alkaline" yiSantai Technologies, amakholomu we-SCX aqashwa ekuhlanzeni amakhompiyutha e-Alkaline. Kulokhu okuthunyelwe, ingxube yezindinganiso ezingathathi hlangothi kanye ne-acidic yayisetshenziswa njengesampula ukuhlola ukusetshenziswa kwamakholomu we-SAX ekuhlanjweni kwe-acidic compound.
Isigaba Sokuhlola
Umdwebo 1. Umdwebo we-Schematic wesigaba esimisiwe oboshwe ebusweni bemidiya ye-SAX HARDATION.
Kulokhu okuthunyelwe, ikholomu ye-SAX kuqala egcwele i-silica ye-quaternary amoren anine yasetshenziswa (njengoba kukhonjisiwe kuMfanekiso 1). Ingxube ye-chromone ne-2,4-dihydroxybenzoic acid yasetshenziswa njengoba isampula ukuze ihlanzwe (njengoba kukhonjisiwe kuMfanekiso 2). Ingxube ichithwe e-methanol futhi ilayishwe ku-cartridge ye-flash ngumjovo. Ukusethwa kokuhlola kokuhlanzwa kwe-Flash kufakwe kuhlu etafuleni 1.
Umdwebo 2. Isakhiwo samakhemikhali sezakhi ezimbili kwingxubevange yesampula.
| Into yokwenza utho | Umshini we-Sepabean ™ t | |||||
| Ama-cartridges | I-4 g sepaflash ejwayelekile Flash Carthridge (i-Ireregular Silica, 40 - 63 μm, 60 Å, Inombolo ye-Order: S-5101-0004) | I-4 g sepaflash BondEd Series Sax Flash cartridge (i-Iroregular Silica, 40 - 63 μm, 60-5001-004-IR) | ||||
| Umbuzi | 254 NM (ukutholwa), 280 nm (ukuqapha) | |||||
| Isigaba | Solvent A: N-HEXANE | |||||
| Solvent b: ethyl acetate | ||||||
| Izinga Lokugeleza | I-30 ml / min | 20 ml / min | ||||
| Ukulayisha isampula | I-20 mg (ingxube yengxenye a nengxenye b) | |||||
| I-gradient | Isikhathi (CV) | Solvent b (%) | Isikhathi (CV) | Solvent b (%) | ||
| 0 | 0 | 0 | 0 | |||
| 1.7 | 12 | 14 | 100 | |||
| I-3.7 | 12 | / | / | |||
| 16 | 100 | / | / | |||
| 18 | 100 | / | / | |||
Imiphumela nengxoxo
Okokuqala, ingxubevange yesampula yahlukaniswa yisigaba esijwayelekile se-flash cartridge pre-egcwele ama-silica ajwayelekile. Njengombukiso kuMfanekiso 3, izakhi ezimbili zesampula zaqoshwa kusuka ku-cartridge kungekudala. Ngokulandelayo, kusetshenziselwe i-sax cartry cartridge ngokuhlanzwa kwesampula. Njengombukiso kuMdwebo 4, i-acid contonent B yagcinwa ngokuphelele ku-SAX CARGCTRED. Ingxenye engathathi hlangothi A yavutha kancane kancane ebhokisini le-cartring elisezingeni lesigaba seselula.
Umdwebo 3. I-flash chromatograph yesampula ku-cartridge ejwayelekile yesigaba ejwayelekile.
Umdwebo 4. I-flash chromatograph yesampula ku-sax cartridge.
Ukuqhathanisa umfanekiso 3 futhi Umdwebo 4, isakhi esiyi-A sinesimo esiphakeme esingahambisani nama-cartridgege amabili ahlukene. Ukuqinisekisa ukuthi i-stuar peak ihambelana nengxenye, singasebenzisa isici esigcwele sokuthwebula i-wavelength esakhelwe kwisoftware yokulawula yomshini we-Sepabean ™. Vula idatha yokuhlola yokwehlukana okubili, Hudula kulayini wesikhombi ku-Chromatograph kuze kube seqoqo eliphakeme kakhulu kanye ne-wavewthleth ephelele yalezi zingxenye ezimbili kuzoboniswa ngokuzenzakalela ngaphansi kwe-chromatograph (njengoba kukhonjisiwe kuMfanekiso 5 noMfanekiso 6). Ukuqhathanisa idatha ephelele ye-wavelength ye-wavecthth yalezi zinto ezimbili, ingxenye i-A inakho i-spectrum yokutholwa okungaguquki ekuhlolweni ezimbili. Ngesizathu sengxenye inesimo esiphakeme esingahambisani nama-cartridge amabili ahlukene, kucatshangelwa ukuthi kunokungcola okukhethekile kwingxenye ye-adji ye-asparid ehlukile kwi-cartridge ejwayelekile yesigaba ne-sax cartridge. Ngakho-ke, ukulandelana okuphelele kwehlukile kwingxenye A nokungcola kula ma-cartridge ama-flart amabili, okuholela emugqeni ophakeme kakhulu kuma-chromatograms.
Umdwebo 5
Umdwebo 6
Uma umkhiqizo oqondiswe kuwo uqoqwe yingxenye engathathi hlangothi a, umsebenzi wokuhlanza ungaphothulwa kalula ngokusebenzisa i-SAX Cartridge ukuze uqedwe ngemuva kokulayishwa kwesampula ngemuva kokulayishwa kwesampula. Ngakolunye uhlangothi, uma umkhiqizo oqondiwe uqoqwe yingxenye ye-acidic B, indlela yokupendela yokuthwebula ingahle yamukelwe ngokulungiswa okuncane kwezinyathelo zokuhlola, lapho isampula ifakwe ngokuphelele nge-Methanol Sollents, shintsha isigaba se-methanol esine-5% acetic acid. I-acetate ion esigabeni seselula izoncintisana nengxenye b yokubopha amaqembu akwa-quaternary amine amaqembu esigabeni se-sax cartridge, ngaleyo ndlela elungisa ingxenye ye-Cartridge ukuze uthole i-cartridge ukuze uthole umkhiqizo oqondiwe. I-Chromatogram yesampula ehlukaniswe ngemodi yokushintshana kwe-Ion yaboniswa kuMfanekiso 7.
Umdwebo 7
Ekuphetheni, isampula ye-acidic noma engathathi hlangothi ingahlanzwa ngokushesha yi-sax cartred ehlanganiswe ne-cartridge evamile yesigaba esisebenzisa amasu okuhlanza ahlukene. Ngaphezu kwalokho, ngosizo lwe-wavelength scanning isici esigcwele esakhelwe kwisoftware yokulawula yomshini we-Sepabean ™.
| Inombolo yento | Usayizi wekholomu | Izinga Lokugeleza (ml / min) | Max.prestrure (PSI / Bar) |
| SW-5001-004-IR | 5.9 g g | 10-20 | 400 / 27.5 |
| SW-5001-012-IR | 23 g | 15-30 | 400 / 27.5 |
| SW-5001-025-IR | 38 g | 15-30 | 400 / 27.5 |
| SW-5001-040-IR | 55 g | 20-40 | 400 / 27.5 |
| SW-5001-080-IR | 122 g | 30-60 | 350 / 24.0 |
| SW-5001-120-IR | 180 g | 40-80 | 300 / 20.7 |
| SW-5001-220-IR | 340 g | 50-100 | 300 / 20.7 |
| SW-5001-330-IR | 475 g | 50-100 | 250 / 17.2
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Ithebula 2 Ukupakisha izinto: I-Silica Ehlanzekile ye-Ultra-Bonder Ereregular, 40 - 63 μm, 60 Å.
Ngeminye imininingwane ngokucaciswa okunemininingwane ye-Sepabean ™Umshini, noma imininingwane yoku-oda ku-Sepaflash Series Flart Cartridges, sicela uvakashele iwebhusayithi yethu.
Isikhathi sePosi: Nov-09-2018