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Kuthiwani ngokuhambisana kwamakholomu e-SepaFlash™ kwamanye amasistimu we-flash chromatography?
OkweSepaFlashTMAmakholomu ochungechunge olujwayelekile, izixhumi ezisetshenzisiwe ziyi-Luer-lock in kanye ne-Luer-slip out. Lawa makholomu angase afakwe ngokuqondile ezinhlelweni ze-ISCO ze-CombiFlash.
Kumakholomu e-SepaFlash HP Series, Bonded Series noma iLOKTM Series, izixhumi ezisetshenzisiwe ziyi-Luer-lock in kanye ne-Luer-lock out. Lawa makholomu angase afakwe ezinhlelweni ze-ISCO ze-CombiFlash kusetshenziswa ama-adaptha engeziwe. Ukuze uthole imininingwane yalawa ma-adaptha, sicela ubheke idokhumenti Santai Adapter Kit ye-800g, 1600g, 3kg Flash Columns.
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Ithini ngempela ivolumu yekholomu yekholomu ye-flash?
Ivolumu yekholomu yepharamitha (i-CV) iwusizo ikakhulukazi ukunquma izici zokukhuphuka. Abanye osokhemisi bacabanga ukuthi ivolumu yangaphakathi ye-cartridge (noma ikholomu) ngaphandle kokupakisha izinto ngaphakathi yivolumu yekholomu. Nokho, umthamo wekholomu engenalutho akuyona i-CV. I-CV yanoma iyiphi ikholomu noma i-cartridge ingumthamo wesikhala esingahlaliwe yimpahla epakishwe ngaphambilini kukholomu. Le volumu ihlanganisa kokubili ivolumu ye-interstitial (ivolumu yesikhala esingaphandle kwezinhlayiya ezigcwele) kanye ne-porosity yangaphakathi yezinhlayiyana (ivolumu yembotshana).
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Uma kuqhathaniswa namakholomu e-silica flash, yikuphi ukusebenza okukhethekile kwamakholomu e-alumina flash?
Amakholomu e-alumina flash angenye inketho lapho amasampula ezwela futhi ethambekele ekonakaleni kujeli ye-silica.
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Injani ingcindezi yangemuva uma usebenzisa ikholomu ye-flash?
Ingcindezi yangemuva yekholomu ye-flash ihlobene nosayizi wezinhlayiyana zezinto ezipakishiwe. Izinto ezipakishwe ezinosayizi wezinhlayiyana ezincane zizoholela ekucindezelweni okuphezulu emuva kwekholomu ye-flash. Ngakho-ke izinga lokugeleza lesigaba seselula esisetshenziswa ku-flash chromatography kufanele sehliswe ngokufanele ukuze kuvinjwe uhlelo lwe-flash ukuthi luyeke ukusebenza.
Ingcindezi yangemuva yekholomu ye-flash iphinde ilingane nobude bekholomu. Umzimba wekholomu omude uzophumela ekucindezelweni okuphezulu emuva kwekholomu ye-flash. Ngaphezu kwalokho, ingcindezi yangemuva yekholomu ye-flash ilingana ngokuphambene ne-ID (ububanzi bangaphakathi) bomzimba wekholomu. Ekugcineni, ingcindezi yangemuva yekholomu ye-flash ilingana ne-viscosity yesigaba seselula esisetshenziswa ku-flash chromatography.
