Ikholomu ye-SepaFlash™

I-Santai Solvent Lines Kit & Izesekeli

Cha, i-silica ene-end-capped ayincibiliki kunoma iyiphi i-solvent ye-organic evame ukusetshenziswa.

Ukuze uthole ukuhlanzeka okuphelele ngamakholomu e-C18, sicela ulandele lezi zinyathelo:
① Hlanza ikholomu ngo-100% wesincibilikisi esiqinile (esiphilayo) se-10 – 20 CVs (ivolumu yekholomu), ngokuvamile i-methanol noma i-acetonitrile.
② Sula ikholomu ngamandla angu-50% + angu-50% anamanzi (uma izithasiselo kudingeka, zifake) ukuze uthole amanye ama-CV angu-3 – 5.
③ Sula ikholomu ngezimo zokuqala zegradient kuma-CV angu-3 – 5.

Ngosayizi wekholomu phakathi kuka-4g no-330g, isixhumi esivamile se-Luer sisetshenziswa kulawa ma-flash columns. Ngosayizi wekholomu ka-800g, 1600g kanye no-3000g, izixhumi ezingeziwe zokuxhuma kufanele zisetshenziselwe ukukhweza lawa makholomu amafuleshi amakhulu ohlelweni lwe-flash chromatography. Sicela ubheke idokhumenti Santai Adapter Kit ka-800g, 1600g, 3kg Flash Columns ukuze uthole imininingwane eyengeziwe.

Kukholomu yesigaba esivamile, kunconywa ukusebenzisa isigaba seselula lapho isilinganiso se-methanol singeqi ku-25%.

Ngokuvamile, kunconywa ukusebenzisa isigaba seselula lapho isilinganiso se-solvents ye-polar singadluli u-5%. Izincibilikisi ze-polar zihlanganisa i-DMSO, i-DMF, i-THF, i-TEA njll.

Ukulayisha isampula eliqinile kuyindlela ewusizo yokulayisha isampula ezohlanzwa kukholomu, ikakhulukazi kumasampuli okuncibilika kancane. Kulokhu, i-ILOK flash cartridge iyisinqumo esifanele kakhulu.
Ngokuvamile, isampuli incibilika ku-solvent efanelekile futhi ikhangiswe ku-adsorbant eqinile engase ifane nesetshenziswa kumakholomu akhanyayo, okuhlanganisa umhlaba we-diatomaceous noma i-silica noma ezinye izinto. Ngemuva kokususwa / ukuhwamuka kwe-solvent eyinsalela, i-adsorbent ibekwa phezu kwekholomu egcwele ingxenye noma ku-cartridge elayishiwe eqinile engenalutho. Ukuze uthole imininingwane eyengeziwe, sicela ubheke idokhumenti yomsebenzisi we-iLOK-SL Cartridge User guide ukuze uthole imininingwane eyengeziwe.

Ivolumu yekholomu icishe ilingane nevolumu efile (VM) uma uziba ivolumu eyengeziwe kumashubhu axhuma ikholomu nomjovo nomtshina.

Isikhathi sokufa (tM) isikhathi esidingekayo ukuze kusetshenziswe ingxenye engagciniwe.

Ivolumu efile (VM) ingumthamo wesigaba seselula esidingekayo ukuze kusetshenziswe ingxenye engagciniwe. Ivolumu efile ingabalwa ngesibalo esilandelayo:VM =F0*tM.

Phakathi kwesibalo esingenhla, i-F0 izinga lokugeleza lesigaba seselula.

Cha, i-silica ene-end-capped ayincibiliki kunoma iyiphi i-solvent ye-organic evame ukusetshenziswa.

Amakholomu e-silica flash ayalahlwa futhi asetshenziswa kanye, kodwa ngokuphathwa kahle, amakhatriji e-silica angasetshenziswa kabusha ngaphandle kokudela ukusebenza.
Ukuze iphinde isetshenziswe, ikholomu ye-silica flash idinga ukumane yomiswe ngomoya ocindezelwe noma ishaywe futhi igcinwe ku-isopropanol.

Isitoreji esifanele sizovumela amakholomu efuleshi e-C18 ukuthi aphinde asetshenziswe:
• Ungalokothi uvumele ikholomu ukuthi yome ngemva kokuyisebenzisa.
• Susa zonke iziguquli eziphilayo ngokugeza ikholomu ngama-80% e-methanol noma i-acetonitrile emanzini ama-CV angu-3 – 5.
• Gcina ikholomu ku-solvent eguquguqukayo eshiwo ngenhla enezixhumo ezisendaweni.

Kumakholomu kasayizi omkhulu ngaphezu kuka-220g, umphumela wokushisa ubonakala ngenqubo yokulingana kwangaphambili. Kunconywa ukusetha izinga lokugeleza ku-50-60% wezinga lokugeleza eliphakanyisiwe kunqubo yokulingana kwangaphambili ukuze kugwenywe umphumela ocacile wokushisa.

Umphumela oshisayo we-solvent exubile usobala kakhulu kune-solvent eyodwa. Thatha i-solvent system cyclohexane/ethyl acetate njengesibonelo, kuphakanyiswa ukuthi kusetshenziswe i-cyclohexane engu-100% kunqubo yokulingana kwangaphambili. Uma ukulinganisa kwangaphambili sekuqediwe, ukuhlolwa kokuhlukanisa kungenziwa ngokuya ngesistimu yokuncibilikisa esethiwe.