Umshini weSepabean ™

Ukulingana kwekholomu kungavikela ikholomu kusuka ekulimaleni ngenxa yomphumela ophumelelayo lapho i-solvent idonsa ngokushesha ikholomu. Ngenkathi i-silica eyomile yangaphambi kwekholomu ithintwa yi-solvent okokuqala ngesikhathi sokuhlukaniswa, ukushisa okuningi kungakhishwa ikakhulukazi lapho i-solvent idonsa ngesilinganiso esiphakeme sokugeleza. Lokhu kushisa kungadala ukuthi umzimba wekholomu ukhubazeke futhi ngenxa yalokho avele ukuvuza okuvela kwikholamu. Kwezinye izimo, lokhu kushisa kungalimaza nesampula elibucayi lokushisa.

Kungenzeka okubangelwa ukuntuleka kwamafutha okuthambisa emgodini wokujikeleza kwepompo.

Inani eliphelele le-tubing yohlelo, oConnetors kanye neChamber yokuxuba cishe i-25 ML.

Iseli yokugeleza yemodyuli yomtshina ingcoliswe yisampula enokutholwa okuqinile kwe-UV. Noma kungenzeka kube ngenxa yokungena kwe-solvent UV yinto ejwayelekile. Sicela wenze okulandelayo kokusebenza:

1

2. I-Solvent UV Inkinga yokutholwa: isib. U-N-Hexane noDCMORomethane (DCM) baqashwa njenge-solvent ye-DCM Sekuvele bamunwe be-DCM ngo-254 nm baphansi kunaleyo ka-N-HEXAWE. Uma kwenzeka kwenzeka le nto, singakwazi ukuyiphatha ngokuchofoza inkinobho ethi "Zero" ekhasini lokuQhunyelwa Lokuhlukaniswa kuhlelo lokusebenza lweSepabean.

3.Ingcingo yokugeleza kwemodyuli yomtshina ingcoliswe kakhulu futhi idinga ukuhlanzwa kwe-ultrasolically.

Kungahle kube ngenxa yalokho izixhumi ezisekhanda eliphethe ikholomu kanye nasengxenyeni eyisisekelo zivunguswe yi-solvent ukuze izixhumi zibambeke.

Umsebenzisi angaphakamisa ngesandla ikhanda lekholomu ngokusebenzisa amandla amancane. Lapho ikhanda lekholomu liphakanyiswa likhuphuke lifinyelele ekuphakameni okuthile, ikhanda lekholamu kufanele likwazi ukuhanjiswa ngokuthinta izinkinobho kulo. Uma ikhanda lekholomu lingeke liphakanyiswe ngesandla, umsebenzisi kufanele athinte ukwesekwa kwezobuchwepheshe kwendawo.

Indlela Ezezimo Eziphuthumayo: Umsebenzisi angafaka ikholomu phezulu kwekhanda lomnikazi wekholomu esikhundleni salokho. Isampula ye-liquid ingafakwa ngqo kukholamu. Ikholomu yokulayisha isampula eqinile ingafakwa ngaphezulu kwekholomu yokuhlukanisa.

1. Amandla aphansi omthombo wokukhanya;

2. Ichibi lokujikeleza lingcolisiwe; Ngezifiso, akukho dolobha eliphakeme noma inani eliphakeme lincane ngokuhlukana, i-Energy Spectra ikhombisa inani elingaphansi kwama-25%.

Sicela ushayele ishubhu nge-solvent efanelekile ku-10ml / min nge-30min bese ubheka i-energy spectrum.if Akukho shintsho ku-spectrum, kubonakala sengathi kunesibani somthombo wokukhanya, sicela ungene esikhundleni sesibani se-deuterium; Uma i-spectrum ishintshile, ichibi lokujikeleza lokujikeleza lingcolisiwe, sicela uqhubeke nokuhlanza nge-solvent efanelekile.

Sicela ubheke ishubhu nesixhumi njalo.

I-wavelength etholwayo isethwe e-wavlength engaphansi kune-245 nm selokhu i-Ethyl Acetate ithola ukumunca okuqinile kuhlu lokutholwa olungaphansi kwe-245NM. I-Baseline Driffing izoba namandla kakhulu lapho kusetshenziswa i-ethyl acetate njengoba nje i-solvent futhi sikhetha i-220 NM njenge-wavelength yokutholwa.

Sicela ushintshe i-wavelength etholwayo. Kunconywa ukukhetha 254nm njenge-wavength etholwayo. Uma i-220 NM ukuphela kwe-wavelength elungele ukutholwa kwesampula, umsebenzisi kufanele aqoqe i-eluent ngokuhlulela ngokucophelela futhi i-solvent eyeqile ingaqoqwa kuleli cala.

Hlanza ikhanda le-solvent ngekhanda ngokuphelele ukuze ususe noma yikuphi ukungcola. Sebenzisa i-ethanol noma i-isopropanol ukuze ugcwalise uhlelo ngokuphelele ukugwema izinkinga ezingenakuqhathaniswa.

Ukuhlanza ikhanda le-solvent filly, qabula isihlungi esivela ekhanda lokuhlunga bese usihlanza ngebhulashi elincane. Bese ugeza isihlungi nge-ethanol futhi ushaye-womisa. Hlanganisa kabusha ikhanda lokuhlunga ukuze usebenzise ikusasa.

Cishe usuka ekuhlukaniseni kwesigaba esijwayelekile ukuze uhlehlise ukwahlukana kwesigaba noma okuphambene nalokho, i-ethanol noma i-isopropanol kufanele isetshenziswe njengoba inguquko i-solvent yokukhipha ngokuphelele noma yiziphi izixazululo ezingenakuqhathaniswa.

Kuphakanyiswa ukusetha isilinganiso sokugeleza ku-40 ml / amaminithi ukufuza imigqa ye-solvent nawo wonke ama-tubings angaphakathi.

Sicela ubeke kabusha phansi komnikazi wekholomu ngemuva kokukhulula isikulufa.

1. Izinga lokugeleza kwesistimu liphezulu kakhulu kwikholomu yamanje ye-flash.

2

3. Enye Isizathu Kubangela Ukuqhekeka Kweshubhu.