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Ungayenza kanjani lapho umsakazo we-solvents ungelutho?
Hlanza ikhanda le-solvent lokuhlunga ngokuphelele ukuze ususe noma yikuphi ukungcola, kungcono ukusebenzisa ukuhlanzwa kwe-ultrasonic.
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Yini ebangela umsindo ophakeme wesisekelo?
1. Iseli yokugeleza komtshina yangcoliswa.
2. Amandla aphansi omthombo wokukhanya.
3. Ithonya lokudonswa kwephampu.
4. Umphumela wokushisa womtshina.
5. Kukhona ama-bubble echibini lokuhlola.
6. Ikholomu noma isigaba sesigaba seselula.
Ku-chromatography elungiselelweni, inani elincane lomsindo wesisekelo linomthelela omncane ekuhlukaniseni.
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Ungayenza kanjani i-Alarm Level Level ngokungajwayelekile?
1. Isixhumi seshubhu ngemuva komshini sikhululekile noma silimele; Faka esikhundleni isixhumi seshubhu;
2. I-Vas Way Check valve yonakalisiwe. Buyisela i-valve yesheke.
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Ungayenza kanjani uma umlando ongumlando ushukumisa
Ngemuva kokuhlukaniswa, kuyadingeka ukulinda imizuzu engu-3-5 ngaphambi kokuvala ukuqiniseka ubuqotho berekhodi lokuhlola.
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Kungani kudingeka silinganise ikholamu ngaphambi kokuhlukana?
Ukulingana kwekholomu kungavikela ikholomu kusuka ekulimaleni ngenxa yomphumela ophumelelayo lapho i-solvent idonsa ngokushesha ikholomu. Ngenkathi i-silica eyomile yangaphambi kwekholomu ithintwa yi-solvent okokuqala ngesikhathi sokuhlukaniswa, ukushisa okuningi kungakhishwa ikakhulukazi lapho i-solvent idonsa ngesilinganiso esiphakeme sokugeleza. Lokhu kushisa kungadala ukuthi umzimba wekholomu ukhubazeke futhi ngenxa yalokho avele ukuvuza okuvela kwikholamu. Kwezinye izimo, lokhu kushisa kungalimaza nesampula elibucayi lokushisa.
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Ungayenza kanjani lapho impompo izwakala kakhulu kunangaphambili?
Kungenzeka okubangelwa ukuntuleka kwamafutha okuthambisa emgodini wokujikeleza kwepompo.
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Yini umthamo wama-tubings nokuxhumeka ngaphakathi kwensimbi?
Inani eliphelele le-tubing yohlelo, oConnetors kanye neChamber yokuxuba cishe i-25 ML.
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Ungayenza kanjani lapho isinali elingesihle liphefumula ku-chromatogram ye-flash, noma i-etuting peak ku-flash chromatograph ingajwayelekile ...
Iseli yokugeleza yemodyuli yomtshina ingcoliswe yisampula enokutholwa okuqinile kwe-UV. Noma kungenzeka kube ngenxa yokungena kwe-solvent UV yinto ejwayelekile. Sicela wenze okulandelayo kokusebenza:
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2. I-Solvent UV Inkinga yokutholwa: isib. U-N-Hexane noDCMORomethane (DCM) baqashwa njenge-solvent ye-DCM Sekuvele bamunwe be-DCM ngo-254 nm baphansi kunaleyo ka-N-HEXAWE. Uma kwenzeka kwenzeka le nto, singakwazi ukuyiphatha ngokuchofoza inkinobho ethi "Zero" ekhasini lokuQhunyelwa Lokuhlukaniswa kuhlelo lokusebenza lweSepabean.
3.Ingcingo yokugeleza kwemodyuli yomtshina ingcoliswe kakhulu futhi idinga ukuhlanzwa kwe-ultrasolically.
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Ungayenza kanjani lapho ikhanda lekholamu lingaphakamisi ngokuzenzakalelayo?
Kungahle kube ngenxa yalokho izixhumi ezisekhanda eliphethe ikholomu kanye nasengxenyeni eyisisekelo zivunguswe yi-solvent ukuze izixhumi zibambeke.
Umsebenzisi angaphakamisa ngesandla ikhanda lekholomu ngokusebenzisa amandla amancane. Lapho ikhanda lekholomu liphakanyiswa likhuphuke lifinyelele ekuphakameni okuthile, ikhanda lekholamu kufanele likwazi ukuhanjiswa ngokuthinta izinkinobho kulo. Uma ikhanda lekholomu lingeke liphakanyiswe ngesandla, umsebenzisi kufanele athinte ukwesekwa kwezobuchwepheshe kwendawo.
Indlela Ezezimo Eziphuthumayo: Umsebenzisi angafaka ikholomu phezulu kwekhanda lomnikazi wekholomu esikhundleni salokho. Isampula ye-liquid ingafakwa ngqo kukholamu. Ikholomu yokulayisha isampula eqinile ingafakwa ngaphezulu kwekholomu yokuhlukanisa.
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Ungayenza kanjani uma ubukhulu bomtshina buba buthakathaka?
1. Amandla aphansi omthombo wokukhanya;
2. Ichibi lokujikeleza lingcolisiwe; Ngezifiso, akukho dolobha eliphakeme noma inani eliphakeme lincane ngokuhlukana, i-Energy Spectra ikhombisa inani elingaphansi kwama-25%.
Sicela ushayele ishubhu nge-solvent efanelekile ku-10ml / min nge-30min bese ubheka i-energy spectrum.if Akukho shintsho ku-spectrum, kubonakala sengathi kunesibani somthombo wokukhanya, sicela ungene esikhundleni sesibani se-deuterium; Uma i-spectrum ishintshile, ichibi lokujikeleza lokujikeleza lingcolisiwe, sicela uqhubeke nokuhlanza nge-solvent efanelekile.
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Ungayenza kanjani lapho umshini uvuza uketshezi ngaphakathi?
Sicela ubheke ishubhu nesixhumi njalo.
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Ungayenza kanjani uma isisekelo siqhubeka sikhuphuka phezulu lapho kuqashwa i-Ethyl Acetate njengoba i-solvent evuthayo?
I-wavelength etholwayo isethwe e-wavlength engaphansi kune-245 nm selokhu i-Ethyl Acetate ithola ukumunca okuqinile kuhlu lokutholwa olungaphansi kwe-245NM. I-Baseline Driffing izoba namandla kakhulu lapho kusetshenziswa i-ethyl acetate njengoba nje i-solvent futhi sikhetha i-220 NM njenge-wavelength yokutholwa.
Sicela ushintshe i-wavelength etholwayo. Kunconywa ukukhetha 254nm njenge-wavength etholwayo. Uma i-220 NM ukuphela kwe-wavelength elungele ukutholwa kwesampula, umsebenzisi kufanele aqoqe i-eluent ngokuhlulela ngokucophelela futhi i-solvent eyeqile ingaqoqwa kuleli cala.
