Imibuzo Evame Ukubuzwa

OkweSepaFlash^TMAmakholomu ochungechunge olujwayelekile, izixhumi ezisetshenzisiwe ziyi-Luer-lock in kanye ne-Luer-slip out. Lawa makholomu angase afakwe ngokuqondile ezinhlelweni ze-ISCO ze-CombiFlash.

Kumakholomu e-SepaFlash HP Series, Bonded Series noma iLOKTM Series, izixhumi ezisetshenzisiwe ziyi-Luer-lock in kanye ne-Luer-lock out. Lawa makholomu angase afakwe ezinhlelweni ze-ISCO ze-CombiFlash kusetshenziswa ama-adaptha engeziwe. Ukuze uthole imininingwane yalawa ma-adaptha, sicela ubheke idokhumenti Santai Adapter Kit ye-800g, 1600g, 3kg Flash Columns.

Ivolumu yekholomu yepharamitha (i-CV) iwusizo ikakhulukazi ukunquma izici zokukhuphuka. Abanye osokhemisi bacabanga ukuthi ivolumu yangaphakathi ye-cartridge (noma ikholomu) ngaphandle kokupakisha izinto ngaphakathi yivolumu yekholomu. Nokho, umthamo wekholomu engenalutho akuyona i-CV. I-CV yanoma iyiphi ikholomu noma i-cartridge ingumthamo wesikhala esingahlaliwe yimpahla epakishwe ngaphambilini kukholomu. Le volumu ihlanganisa kokubili ivolumu ye-interstitial (ivolumu yesikhala esingaphandle kwezinhlayiya ezigcwele) kanye ne-porosity yangaphakathi yezinhlayiyana (ivolumu yembotshana).

Amakholomu e-alumina flash angenye inketho lapho amasampula ezwela futhi ethambekele ekonakaleni kujeli ye-silica.

Ingcindezi yangemuva yekholomu ye-flash ihlobene nosayizi wezinhlayiyana zezinto ezipakishiwe. Izinto ezipakishwe ezinosayizi wezinhlayiyana ezincane zizoholela ekucindezelweni okuphezulu emuva kwekholomu ye-flash. Ngakho-ke izinga lokugeleza lesigaba seselula esisetshenziswa ku-flash chromatography kufanele sehliswe ngokufanele ukuze kuvinjwe uhlelo lwe-flash ukuthi luyeke ukusebenza.

Ingcindezi yangemuva yekholomu ye-flash iphinde ilingane nobude bekholomu. Umzimba wekholomu omude uzophumela ekucindezelweni okuphezulu emuva kwekholomu ye-flash. Ngaphezu kwalokho, ingcindezi yangemuva yekholomu ye-flash ilingana ngokuphambene ne-ID (ububanzi bangaphakathi) bomzimba wekholomu. Ekugcineni, ingcindezi yangemuva yekholomu ye-flash ilingana ne-viscosity yesigaba seselula esisetshenziswa ku-flash chromatography.

Vula insimbi bese ulinda umlayezo othi “Ulungile”. Qiniseka ukuthi uxhumano lwenethiwekhi ye-iPad lulungile, futhi umzila uvuliwe.

Hlola futhi uqinisekise isimo somzila ukuze wenze isiqiniseko sokuthi i-iPad ingaxhunywa kumzila wamanje.

Ukulinganisa kwenziwa lapho ikholomu imanziswe ngokuphelele futhi ibukeka iguquguqukayo. Ngokuvamile lokhu kungenziwa ngokushayela ama-CV angu-2 ~ 3 wesigaba seselula. Phakathi nenqubo yokulinganisa, ngezinye izikhathi singathola ukuthi ikholomu ayikwazi ukumanziswa ngokuphelele. Lokhu kuyinto evamile futhi ngeke kuphazamise ukusebenza kokuhlukanisa.

Hlola ukuthi i-tube rack ibekwe ngendlela efanele yini endaweni efanele. Uma lokhu sekwenziwe, isikrini se-LCD ku-tube rack kufanele sibonise uphawu oluxhunyiwe.

Uma i-tube rack inephutha, umsebenzisi angakhetha i-tube rack eyenziwe ngokwezifiso ohlwini lwamashubhu ku-SePaBean App ukuze isetshenziswe okwesikhashana. Noma xhumana nonjiniyela wangemva kokudayiswa.

Hlola ukuthi ibhodlela le-solvent liyashoda yini i-solvent ehlobene futhi ugcwalise isincibilikisi.

Uma ulayini we-solvent ugcwele i-solvent, sicela ungakhathazeki. Ibhamuza lomoya alithinti ukuhlukaniswa kwefuleshi njengoba lingenakugwemeka phakathi nokulayisha isampula eliqinile. Lawa mabhamuza azokhishwa kancane kancane ngesikhathi sokuhlukaniswa.

Sicela uvule ikhava yangemuva yensimbi, hlanza i-pump piston rod nge-ethanol (ukuhlaziywa kokucwenga noma ngaphezulu), bese uzungezisa ipiston ngenkathi ugeza kuze kube yilapho ipiston ijika kahle.

1. Insimbi ngeke ikwazi ukumpompa izincibilikisi lapho izinga lokushisa lendawo lingaphezu kuka-30℃, ikakhulukazi izincibilikisi ezibilayo eziphansi, Njenge-dichloromethane noma i-Ether.

Sicela uqinisekise ukuthi izinga lokushisa le-ambient lingaphansi kuka-30℃.

2. Umoya uthatha ipayipi ngenkathi i-instrumnet ingasebenzi isikhathi eside.

Sicela wengeze i-ethanol ku-ceramic rod yekhanda lepompo (ukuhlaziywa kokucwenga noma ngaphezulu) futhi ukhuphule izinga lokugeleza ngesikhathi esifanayo. Isixhumi esiphambi kwepompo sonakalisiwe noma I-Lose, lokhu kuzobangela ukuthi ulayini uvuze umoya .Sicela uhlole ngokucophelela ukuthi ingabe ukuxhunywa kwepayipi kukhululekile yini.

3. Isixhumi esiphambi kwepompo sonakalisiwe noma i-Lose, sizokwenza ukuthi umugqa uvuze umoya.

Sicela uqinisekise ukuthi isixhumi sepayipi sisesimweni esihle yini.

I-valve yokuqoqa ivinjiwe noma iyaguga. Sicela ushintshe i-valve ye-solenoid enezinhlangothi ezintathu.

ISELULEKO: Sicela uxhumane nonjiniyela wangemuva kokudayiswa ukuze ubhekane nakho.