Amazolumbo

USantai Solvent Lines Kit & izesekeli

Cha, i-silica ephelile eboshwe i-infoluble kunoma iyiphi i-organic solvent evame ukusetshenziswa.

Ngokuhlanjululwa okuhle nge-C18 Flash Columns, sicela ulandele lezi zinyathelo:
① Flush ikholomu nge-100% ye-solvent eqinile (ye-organic) ye-solvent yama-10 - 20 ama-CVs (ivolumu yekholomu), imvamisa ye-methanol noma i-acetonitrile.
② Flush ikholomu nge-50% enamandla + 50% aqued (uma izengezo zidingeka, zibandakanya nazo) kwamanye ama-3 - 5 ama-CV.
③ Flush ikholomu ngezimo zokuqala ze-gradient ama-3 - 5 ama-CV.

Ngosayizi wekholomu phakathi kwe-4G ne-330G, isixhumi esijwayelekile se-LEERS sisetshenziswa kulezi zinkundla ze-flash. Ngosayizi wekholomu ka-800g, 1600g ne-3000g, ama-adap we-Connector angeziwe kufanele asetshenziselwe ukukhweza lezi zinhle ezinkulu ze-flash kuhlelo lwe-chromatography. Uyacelwa ukuthi ubheke kudokhumenti ye-Santai Adapter Kit ye-800g, 1600g, amakholomu we-3kg flash ukuthola eminye imininingwane.

Kwikholamu yesigaba ejwayelekile, kunconywa ukusebenzisa isigaba se-mobile lapho isilinganiso se-methanol singadluli ku-25%.

Ngokuvamile, kunconywa ukusebenzisa isigaba se-mobile lapho isilinganiso se-polar solvents esingadluli ku-5%. I-polar solvents ifaka i-DMSO, DMF, THF, itiye njll.

Ukulayisha isampula eqinile kuyindlela ewusizo ukulayisha isampula ukuze ihlanzwe kwikholamu, ikakhulukazi kumasampula we-solubity ephansi. Kulokhu, iLok Flash Carthridge iyisinqumo esifanele kakhulu.
Ngokuvamile, isampula lichithwa kwi-solvent efanelekile futhi i-adsorbed ku-adsorbant eqinile engafana ne-flash colomns, kufaka phakathi umhlaba we-diatomaceous noma ezinye izinto. Ngemuva kokususwa / ukuhwamuka kwe-solvent eyakhayo, ama-adsorbent afakwa ngaphezulu kwekholomu egcwele ngokwengxenye noma ebhokisini eliqinile eliqinile. Ngemininingwane enemininingwane eminingi, sicela ubheke kudokhumenti i-ILok-SL Cartridge Widle Sindies ukuthola eminye imininingwane.

IVolumu yekholomu icishe ilingane nevolumu efile (VM) lapho inganaki ivolumu eyengeziwe kumagama axhumanisa ama-tubings axhuma ikholomu nesijobelelo kanye nomtshina.

Isikhathi Esifile (i-TM) isikhathi esidingekayo sokuqedwa kwengxenye engafundisiwe.

IVolumu Efile (VM) ivolumu yesigaba seselula esidingekayo ekuqedeni ingxenye engafakwanga. Ivolumu efile ingabalwa yi-equation elandelayo: VM = F0 * TM.

Phakathi kokulingana okungenhla, i-F0 yisilinganiso sokugeleza kwesigaba seselula.

Cha, i-silica ephelile eboshwe i-infoluble kunoma iyiphi i-organic solvent evame ukusetshenziswa.

Amakholomu we-Silica Flash asetshenziswa futhi asetshenziswa okukodwa, kepha ngokuphathwa okufanele, ama-cartridge ama-silica angasetshenziswa ngaphandle kokuzidela ukusebenza.
Ukuze asetshenziswe kabusha, ikholomu ye-Silica Flash idinga ukumiswa ngomoya ocindezelwe noma igcwele futhi igcinwe ku-isopropanol.

Isitoreji esifanele sizovumela amakholomu we-C18 Flash ukuthi asetshenziswe kabusha:
• Ungalokothi uvumele ikholomu ukuthi yome ngemuva kokusebenzisa.
• Susa wonke ama-organic modifiers ngokufaka ikholomu nge-80% ye-methanol noma i-acetonitritle emanzini ama-3 - 5 ama-CV.
• Gcina ikholomu kuleli ngenhla elishiwo ngenhla elibunjiwe ngokufakelwa kokuphela endaweni.

Kumakholomu amakhulu asayizi ngaphezulu kwama-220g, umphumela we-thermal usobala kwinqubo yokulingana kwangaphambili. Kunconywa ukusetha isilinganiso sokugeleza ku-50-60% wesilinganiso sokugeleza esiphakanyisiwe kwinqubo yangaphambi kokulingana ukugwema umphumela osobala we-thermal.

Umphumela we-thermal we-solvent exubile usobala ukwedlula i-solvent eyodwa. Thatha uhlelo lwe-solvent cyclohexenane / ethyl acetate njengesibonelo, kuphakanyiswa ukuthi usebenzise i-100% cyclohekhali yenqubo yangaphambi kokulingana. Lapho kuqedwa ukulingana kwangaphambilini, ukuhlolwa kwehlukana kungenziwa ngokuya ngohlelo lwe-preset solvent.