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Isicelo se-R & D
Intshayelelo
Iintsholongwane yiklasi ye-metabolites yesibini eziveliswe ziintsholongwane (kubandakanya intsholongwane, i-fungi, i-actooyomycetes) okanye imichiza eyahlukileyo. Iintsholongwane zinokuthintela ukukhula kunye nokusinda kwezinye ii-microorganisosms. I-atvibiotic yokuqala efunyenwe ngumntu, i-penicillin, yafunyanwa ngu-Alexander waseBritani ngo-1928. Wabona ukuba intsholongwane kwindawo ekufutshane nayo ayinakukhula kwisitya senkcubeko engcolisekileyo. Uye wathumela ukuba ubumba kufuneka bube ngumsebenzi wentsholongwane, awawubiza ngokuba nguPenicillin ngo-1928. Nangona kunjalo, izithako ezisebenzayo zazingahlanjululwanga ngelo xesha. Ngo-1939, i-ersts Chain kunye neHoward Florey yeYunivesithi yase-Oxford bagqibe kwelokuba aphuhlise isiyobisi esinokunyanga usulelo lwentsholongwane. Emva koqhakamshelalelo lokunxibelelana ukuze ufumane ubunzima, bakhupha ngempumelelo kwaye batyhidiwe ngempumelelo nepenicillin ephumeleleyo kwimigca. Ngophuhliso lwabo oluyimpumelelo lwePenicillin njengechiza lonyango, amacebo, i-chain kunye neflorey i-romey yebhaso le-1945 kunyango.
Iintsholongwane zisetyenziswa njengabameli be-antibacterial ukunyanga okanye ukuthintela usulelo lwe-bactery. Kukho iindidi ezininzi eziphambili ze-antibiotics ezisetyenziswa njengeearhente ze-antibatics: I-Semi-synthesis kunye neSynthesis iyonke. Ii-antibiotics eziveliswe ngulwelo lwendalo kufuneka zilungiswe ngokweendlela zekhemikhali ngenxa yokuzinza kwemichiza, iziphumo ebeziyityhefu, i-antibacterial kunye neminye imiba. Emva kokuguqulwa kwemichiza, ii-antibiotics zinokufumana uzinzo ongezelelweyo, zincitshiswe iziphumo ebezingalunganga, zinciphise i-aspicterium ye-aspicterium, inciphise ukuxhaswa kwe-aspitacterium, inciphise ukuxhaswa kwe-aspitacterium, inciphise i-biaricterium, inciphise i-biaring yeziyobisi, iphuculwe i-biaaaaasaaction, kwaye ngaloo ndlela iphuculweyo yonyango lweziyobisi. Ke ngoko, i-syli-syntatic intsholongwane okwangoku yeyona ndlela ithandwayo ekuphuhlisweni kwamayeza e-antibiotic.
Ukuphuhliswa kwe-syntitic ye-syntitic, ii-antibiotics zinepropathi yokucoceka okusulekileyo, iimveliso ezininzi kunye nezinto ezinzima kuba zivela kwiimveliso zentsholongwane. Kule meko, uhlalutyo kunye nolawulo lokungcola kwi-semi-synthetics eshukumisayo kubalulekile. Ukwenzela ukuba uchonge kwaye ubonakalise ukungcola, kuyimfuneko ukufumana inani elaneleyo lokungcola kwimveliso ye-semi-synthetic. Phakathi kweendlela ezisetyenziswayo ezisetyenziswayo ezisetyenziswayo, i-flash chromatography yindlela esebenzayo enezibonelelo ezifana nesampulu yesampulu yesampulu, indleko ephantsi, ukugcinwa kwexesha, njl njl.
In this post, the main impurity of a semi-synthetic aminoglycoside antibiotic was utilized as the sample and purified by a SepaFlash C18AQ cartridge combined with the flash chromatography system SepaBean™ machine. Impembelelo yeMveliso yeNgxelo yeNgxelo ifunyenwe ngempumelelo, iphakamisa isisombululo esifanelekileyo sokuhlanjululwa kwezi zinto.
Icandelo lovavanyo
Isampulu yanikezelwa ngobubele yinkampani yamayeza yasekhaya. Isampulu yayiluhlobo lwe-Amino Polycyclic Carbohydrates kunye nesakhiwo saso semolekyuli sasifana ne-Aminiibiotics. I-polared yesampulu yayiphezulu kakhulu, iyenza inyibilikiswe emanzini. Umzobo ocwangcisiweyo wesampulu yemolekyuli yaboniswa kuMfanekiso 1. Ukucoceka kwesampulu ye-RAW yayimalunga ne-88% njengoko kuhlaziywa yi-HLLC. Ukuhlanjululwa kwezi zinto zenzala ephezulu, isampulu iya kugcinwa ngokungafanelekanga kwikholamu yesiqhelo ye-C18 ngokwamava aselwe ngaphambili. Ke ngoko, ikholamu ye-C18AQ yaqashwe ukuba kuhlanjululwe isampulu.
Umzobo 1. Umzobo we-schematic wesampulu yemolekyuli.
Ukulungiselela isisombululo sesampulu, isampulu ye-MG CREUDE yachithwa kwi-5 ml amanzi kwaye emva koko yayenzelwe ukuba ibe sisisombululo esicacileyo ngokupheleleyo. Isisombululo sesampulu emva koko satyalwa kwikholamu yokuKhanya yintsholongwane. Ukusetwa kokuvavanywa kokuhlanjwa kweFlash kudweliswe kwiTheyibhile 1.
| Isixhobo | Yahlulahlula umatshini 2 | |
| Iikhatriji | I-12 g eyahlula i-c18aq rp i-caltridge (i-spherical silica, 20 μ, inombolo ye-RW-5222-012-SP (AQ) | |
| Wavelength | I-204 NM, i-220 NM | |
| Isigaba sefowuni | I-Solvent A: Amanzi I-Solvent B: Acetostitrile | |
| Umgangatho wohambo | I-15 ml / min | |
| Isampulu yokulayisha | I-50 mg | |
| Igradient | Ixesha (imiz) | I-Solvent B (%) |
| 0 | 0 | |
| 19.0 | 8 | |
| I-47.0 | 80 | |
| I-52.0 | 80 | |
Iziphumo neengxoxo
I-flash chromatogram yesampulu kwi-C18aq Cartridge iboniswe kumzobo 2. Njengoko kubonisiwe kumzobo 2, isampulu ye-Polar yayigcinwa ngokufanelekileyo kwi-C18AQ ye-cartridge. Emva kwe-LOOPONIC yokuqokelelwa kwamaqhezu aqokelelweyo, imveliso ekujolise kuyo yayinobunyulu be-96.2% (njengoko kubonisiwe kumzobo 3) ngohlalutyo lwe-HPPC. Iziphumo zibonakalise ukuba imveliso ecocekileyo inokupheliswa ngakumbi kuPhando olulandelayo loPhando kunye noPhuculo.
Umzobo 2. I-Flash Chromatogram yesampulu kwikhatriji ye-C18AQ.
Umzobo 3. I-HPPC yeClromatom yemveliso ekujoliswe kuyo.
Ukuqukumbela, ngokwahlula-hluha c18aq RP ye-carridge edibene nenkqubo ye-flash chromatography yesahlulo yesahlulo esikhawulezayo kunye nesomeleleyo sokuhlanjwa kweesampulu zepolir.
Malunga ne-PopfffLash C18aq rp carridges
Kukho uthotho lwezahlulo ze-P18aq rp carridges eneenkcukacha ezahlukeneyo ezivela kwiTekhnoloji ye-SAntai (njengoko kubonisiwe kwiTheyibhile 2).
| Inombolo yento | Ubungakanani bekholamu | Umgangatho wohambo (ml / min) | Max.pressored (i-psi / bar) |
| SW-5222-004-SP (AQ) | 5.4 g | 5-15 | I-400 / 27.5 |
| SW-5222-012-SP (AQ) | I-20 g | I-10-25 | I-400 / 27.5 |
| SW-5222-025-SP (AQ) | 33 g | I-10-25 | I-400 / 27.5 |
| SW-5222-040-SP (AQ) | 48 g | I-15-30 | I-400 / 27.5 |
| SW-5222-080-SP (AQ) | I-105 g | 25-50 | I-350 / 24.0 |
| SW-5222-120-SP (AQ) | I-155 g | I-30-60 | I-300 / 20.7 |
| SW-5222220-SP (AQ) | I-300 g | 40-80 | I-300 / 20.7 |
| SW-5222-330-SP (AQ) | I-420 g | 40-80 | I-250 / 17.2 |
Itheyibhile 2. Yahlula i-c18aq rp carridges. Izinto zokupakisha: Ukusebenza kakuhle kwe-SP18 (AQ) ye-AICACA, 45 μm, 100 å.
Ngolwazi oluthe kratya malunga neenkcazo ezichaziweyo zomatshini we-Supben ™, okanye ulungelelwaniso lwe-odolo malunga ne-Prefish Carridges, nceda undwendwele iwebhusayithi yethu.
Ixesha lokuposa: Oct-26 ukuya kwi-2618