Rui Huang, Bo xu
Isicelo se-R & D
Intshayelelo
I-ion yokutshintshiselana i-chromatography (IEC) yindlela ye-chromatographic ye-chromatographic eqhelekileyo esetyenziselwa ukwahlula kwaye icoce imimandla eboniswe kwifom ye-Ionic kwisisombululo. Ngokutsho kwentlawulo eyahlukileyo ye-ion etshintshe i-IEC, i-IEC inokwahlulwa ibe ziindidi ezimbini, i-creation yokutshintshiselana i-chromatography kunye ne-chromatography ye-chromatography. KwiCosta Tshintshiselwano yeChromatography, amaqela e-acidic adityaniswe kumphezulu wemithombo yeendaba yokwahlukana. Umzekelo, i-Sulfonic Acid (-SO3H) liqela elisetyenzisiweyo elisetyenzisiweyo kwi-CARD eqinileyo Kwi-anyanisi yokutshintshiselana i-chromatography, amaqela eAlkaline adityaniswe kumphezulu wamajelo eendaba. Umzekelo, i-amine yethutyana
Phakathi kweemveliso zendalo, i-flavonoids itsalele ingqalelo yabaphandi ngenxa yendima yabo kuthintelo kunye nonyango lwezifo zentliziyo. Kuba iimolekyuli ze-flavonoid ziyi-acidic ngenxa yobukho bamaqela e-phenolic ye-phenolic, i-ion yokutshintshiselana i-chromatography yenye indlela yokwahlulahlula iSigaba esiqhelekileyo se-chromatography yokwahlulahlula kunye nokucocwa kwezi ntlobo ze-acidic. Kwi-flash chromatography, iMidiya yeMidiya esetyenzisiweyo ye-ion yotshintshiselwano yi-silica gel matrix apho amaqela atshintshiselana khona. Ezona ndlela zisetyenziswa rhoqo i-ion esetyenziswayo kwi-flash chromatography yi-Scx (ihlala i-Sulfonis Acid) kunye ne-sax (ihlala iQuenary Amine Group). Kwinqaku lesicelo esishicilelweyo ngaphambili ngesihloko esithi "Ukusetyenziswa kwesakhiwo se-cern ezamahala ikhomishini ye-Alkaline, iikholamu zeSantai zaziqashwe ukuhlanjululwa kweAlkaline. Kule thuba, imigangatho yokungathathi hlangothi kunye ne-acidic yayisetyenziswa njengesampulu yokuphonononga ukusetyenziswa kweekholakisi ze-SAX ekuhlanjululweni kwee-acidic.
Icandelo lovavanyo
Figure 1. The schematic diagram of the stationary phase bonded to the surface of SAX separation media.
Kule posi, ikholamu ye-SAX ipakishwe kwangaphambili kunye ne-quatertary Amine Bold Silica isetyenzisiwe (njengoko kubonisiwe kumzobo 1). Umxube we-chromone kunye ne-2,4-dihydroxybnoic acid yasetyenziswa njengesampulu ukuze ihlanjululwe (njengoko kubonisiwe kumzobo 2). Umxube waphulukana ne-methanol kwaye ilayishwe kwi-carridge ye-flash yintsholongwane. Ukuseta uvavanyo lovavanyo lokuhlanjwa kweFlash kudweliswe kwiTheyibhile 1.
Umzobo 2. Ubume bemichiza yezinto ezimbini kumxube wesampulu.
| Isixhobo | Yahlulahlula umatshini t | |||||
| Iikhatriji | I-4 g eyahlule i-Flash Cartridge (i-Argregate Silica, 40 - 63 μm, 60 å, inombolo: Inombolo ye-S-5101-0004) | I-4 g eyahlulahlula i-Sax Flash Cartridge (i-Argonver Silica, 40 - 6, i-60 å-5001-00-ir) | ||||
| Wavelength | I-254 NM (ihloliwe), i-280 NM (iliso) | |||||
| Isigaba sefowuni | I-Solvent A: N-Hexane | |||||
| I-Solvent B: I-Ethyl Acetate | ||||||
| Umgangatho wohambo | I-30 ml / min | I-20 ml / min | ||||
| Isampulu yokulayisha | I-20 mg (umxube wecandelo A kunye neCandelo B) | |||||
| Igradient | Ixesha (cv) | I-Solvent B (%) | Ixesha (cv) | I-Solvent B (%) | ||
| 0 | 0 | 0 | 0 | |||
| 1.7 | 12 | 14 | I-100 | |||
| 3.7 | 12 | / | / | |||
| 16 | I-100 | / | / | |||
| 18 | I-100 | / | / | |||
Iziphumo neengxoxo
Okokuqala, umxube wesampulu wahlukaniswa yi-carridge yesigaba esiqhelekileyo esipakishwe kwangaphambili nge-silica eqhelekileyo. Njengoko kubonisa kumzobo 3, ezi zinto zimbini kwisampulu zazibhalwe kwikhatriji enye emva kwenye. Emva koko, i-carridge ye-sax flash yasetyenziselwa ukuhlanjululwa kwesampulu. Njengoko kubonisa kumzobo 4, icandelo le-acidic B lagcinwa ngokupheleleyo kwi-cartridge. Inxalenye engathathi hlangothi i-Edrid Add kwi-carridge ngesigaba sezixhobo.
Umzobo 3. I-chromatom ye-chromatom yesampulu kwi-cartridge yenqanaba eliqhelekileyo eliqhelekileyo.
Umzobo we-4
Ukuthelekisa umzobo 3 kunye ne-4, icandelo eline-Asment engahambelaniyo kwi-Peak ye-Peak kwii-carridges ezimbini ezahlukeneyo. Ukuqinisekisa ukuba i-Pepy Peak ihambelana necandelo, sinokusebenzisa inqaku elipheleleyo le-wavency yesaphulelo eyakhelwe kwisoftware yolawulo lwamatshini wokuLawulwa kwe-sadbean ™. Vula idatha yovavanyo yendlela ezimbini, tsala i-Abreyimu kwi-Axis kwi-Axis (CV) kwi-chromatom yendawo ephezulu ye-Elpres ehambelana ne-chromatom (njengoko kubonisiwe kumzobo we-5 kunye ne-6). Ukuthelekisa i-Wavect Spectrum epheleleyo yedatha yale nqanaba zimbini, icandelo a line-spectrum engaguqukiyo kwiimvavanyo ezimbini. Ngesizathu secandelo anomonge we-Peak ongenamdla kwii-carridges ezimbini ezahlukeneyo, icingelwa ukuba kukho ukungcola okuthile kwicandelo elinendawo eyahlukileyo kwi-cartridge yenqanaba eliqhelekileyo kunye ne-cartridge. Ke ngoko, ukulandelelana kokukhokelela kwahlukile kwicandelo a kunye nokungahlambuluki kwezi zixhobo zimbini zebhokisi, zibangela ubume be-chromatoograms.
Umzobo 5. I-wavelength epheleleyo ye-suveclor yecandelo a kunye nokungalungisi kwahlulwe yi-cartridge yenqanaba eliqhelekileyo.
Umzobo 6. I-Wavelength egcweleyo yecandelo lecandelo a kunye nokungalungisi kwahlulwe yi-cartridge.
Ukuba imveliso ekujoliswe kuyo iqokelelwe yinxalenye engesopolitiko A, umsebenzi wokucoca unokugqitywa ngokulula ngokusebenzisa ikhaphethi ye-sax emva kokulayishwa kwesampulu. Kwelinye icala, ukuba imveliso ekujoliswe kuyo iqokelelwe yinxalenye ye-Acidic B, indlela yokukhululwa-yokukhululwa kwayo inokuguqulwa kuphela kwi-cartridge encinci kwi-SINTACS, tshintsha iSigaba seSigaba esiqhelekileyo esine-5% ye-acetic acid. I-icetate i-ion kwiSigaba seSoftware iya kukhuphisana necandelo le-B lokubopha amaqela e-Amine ion kwiSigaba se-Sax Cartridge, ngaloo ndlela yakhupha icandelo B ukusuka kwi-cartridge ukufumana imveliso ekujolise kuyo. I-chromatogram yesampulu eyahlulwe kwimodi yotshintshiselwano ye-ION iboniswe kuMfanekiso 7.
Umzobo we-7
Ukuqukumbela, isampulu ye-acidic okanye ukungathathi cala kungazihlanjululwa ngokukhawuleza yi-cartridge edityaniswe ne-cartridge isebenzisa iindlela ezahlukeneyo zokucoca. Ngaphaya koko, ngoncedo lwezinto ezigcweleyo ezigcweleyo ezakhelwe kwiSoftware yoLawulo ye-Subbean ™, i-Surction Spect Spectum ye-Edperment yenziwa ngokulula kwaye incedise amaqhezu aphucukileyo ngokukhawuleza kwaye ke ukuphucula ukusebenza kakuhle.
| Inombolo yento | Ubungakanani bekholamu | Umgangatho wohambo (ml / min) | Max.pressored (i-psi / bar) |
| SW-5001-004-IR | 5.9 g | I-10-20 | I-400 / 27.5 |
| SW-5001-012-IR | 23 g | I-15-30 | I-400 / 27.5 |
| SW-5001-025-ir | 38 g | I-15-30 | I-400 / 27.5 |
| SW-5001-040-IR | I-55 g | 20-40 | I-400 / 27.5 |
| SW-5001-080-ir | 122 g | I-30-60 | I-350 / 24.0 |
| SW-500-10-IR | I-180 g | 40-80 | I-300 / 20.7 |
| I-SW-50010-IR | I-340 g | I-50-100 | I-300 / 20.7 |
| I-SW-5001-30-ir | I-475 g | I-50-100 | I-250 / 17.2
|
Itheyibhile 2. Yahlula-hlula uthotho lwe-sax flash cartridges. Izinto zokupakisha: I-Ultra-Ecnary Sax-Bold Silica, 40 - 63 μm, 60 å.
Ngolwazi oluthe kratya malunga neenkcazo ezichaziweyo zeparaben ™umatshini, okanye ulungelelwaniso lwe-odolo malunga ne-Prayflash Seripriges ye-carshid, nceda undwendwele iwebhusayithi yethu.
IXESHA LOKUQALA: UNv-09-2018