Rui Huang, Bo xu
Isicelo se-R & D
Intshayelelo
I-peptide yindawo edityaniswe yi-Amino Acids, nganye ineempawu ezizodwa zomzimba kunye neekhemikhali ngenxa yeentlobo ezahlukeneyo kunye ne-odolo yentsalela ye-Amino Acid ebonisa ukulandelelana kwayo. Ngophuhliso lweSigaba seSigaba seSigaba seSigaba seSigaba seSigaba seSigaba seSigaba seSigaba seSigaba seSigaba seSigaba seSigaba seSigaba seSigaba seSigaba seSigaba seSigaba seSigaba seSigaba seSigaba seSigaba seSigaba seSigaba seZikhephethi seZikhephe ezahlukeneyo zenze inkqubela phambili enkulu. Nangona kunjalo, ngenxa yokuqulunqwa okuntsonkothileyo kwePeptide ifunyenwe yiSigaba seSigaba seSigaba esiqinileyo, imveliso yokugqibela kufuneka ihlanjululwe ngeendlela zokwahlulahlulahlula. Iindlela ezisetyenziswayo ezisetyenziselwa ukuhlambulula kwe-peptides ziqokelela i-ion yotshintshiselwano (i-IEC) kunye neSigaba sokuCima kweSigaba sokuSebenzisa i-Molecule (i-TPLC), ityala eliyi-1 kda lalipapashwe ngaphambili yi-santai Itekhnoloji yeTekhnoloji, apho i-RP ye-RP ye-CARPRG isetyenziselwe ukuhlanjululwa okukhawulezileyo kwe-thymontle (i-TP-5) kunye neNtlanganiso yeNgxelo yeMveliso ifunyenweyo.
Umzobo 1. Ii-Amino eziqhelekileyo ze-Amino Acids (iphinde zaveliswa kwiwebhusayithi ethi: www.b.com).
Kukho iintlobo ezingama-20 ze-amino acid ezixhaphakileyo ekufumaneni i-peptides. Ezi acino acid zinokwahlulwahlulwahlulwa la maqela alandelayo ngokwepropathi yabo ye-Acid kunye ne-Acid-Cellise Ukulandelelana kwePeptide, ukuba i-Amino Acids yenza ulandelelwano lwe-polar (njengoko iphawulwe ngombala wepinki kumzobo 1), i-Glutamine, i-rinetine, i-typle, i-typutine, inyibilikiswe emanzini. Ngexesha lenkqubo yokucoca kwezi zimo ezomeleleyo ze-Polar Pwangcisiweyo kwi-chromatography-School, i-phenomenon ebizwa ngokuba yi-Hydrophologic Disct ye-Shydrophoblogic ye-Shydrophobic, i-AQ iSigaba seSigaba seHromatography, i-AQ iSigaba seSigaba seChromatography kunye nezicelo zabo). Xa kuthelekiswa neekholamu eziqhelekileyo ze-C18, iikholamu eziphuculweyo ze-C18aq zilungele ukuhlanjululwa kwepolar okanye iisampulu ze-hydrophilic. Kule posi, isiporho esomeleleyo sepollation sasetyenziswa njengesampulu kwaye ihlanjululwe yikholam ye-C18AQ. Ngenxa yoko, iNtleko yeMveliso ekujoliso kuyo ifunyenwe kwaye inokusetyenziswa kolu phando kunye nophuhliso.
| Isixhobo | Yahlula™umatshini 2 | |||
| Iikhatriji | I-12 g eyahlula i-clash cartridge (20 - 45 μm, 100 μmbre: i-MW-5222-012-SP) | I-12 g eyahlula i-CARPRICRge Cartridge (20 - 45 μm, 100 μm, inombolo yocwangco: i-SW-5222- 0) | ||
| Wavelength | I-254 NM, i-220 NM | I-214 NM | ||
| Isigaba sefowuni | I-Solvent A: Amanzi I-Solvent B: Acetostitrile | |||
| Umgangatho wohambo | I-15 ml / min | I-20 ml / min | ||
| Isampulu yokulayisha | I-30 mg | |||
| Igradient | Ixesha (cv) | I-Solvent B (%) | Ixesha (imiz) | I-Solvent B (%) |
| 0 | 0 | 0 | 4 | |
| 1.0 | 0 | 1.0 | 4 | |
| 10.0 | 6 | 7.5 | 18 | |
| 12.5 | 6 | 13.0 | 18 | |
| 16.5 | 10 | 14.0 | 22 | |
| 19.0 | 41 | 15.5 | 22 | |
| 21.0 | 41 | 18.0 | 38 | |
| / | / | 20.0 | 38 | |
| 22.0 | 87 | |||
| 29.0 | 87 | |||
Iziphumo neengxoxo
Ukuthelekisa ngokusebenza kokucoca isampulu ye-polar peplate phakathi kwekholamu ye-C18 ye-C18AQ, sisebenzise ikholamu ye-C18, sisebenzise ikholamu ye-C18 ye-C18 yokuhlanjululwa kwe-flash yesampulu. Njengoko kubonisiwe kumzobo 2, ngenxa yokuwa kwinqanaba lesigaba se-h18 ebangelwa ngumyinge omkhulu we-CATEATE, isampulu yayingayigcinile i-CARPRIDRE ye-C18 eqhelekileyo kwaye yakhutshwa ngokuthe ngqo kwinqanaba lesibini. Ngenxa yoko, isampulu yayingahlukanga ngokufanelekileyo kwaye ihlanjululwe.
Umzobo 2
Okulandelayo, sasebenzisa ikhomishini ye-C18AQ yokuhlanjululwa kweflethi kwisampulu. Njengoko kubonisiwe kwi-3, iPeptimide yayigciniwe ngempumelelo kwikholamu emva koko yaphuma. Imveliso ekujoliswe kuyo yahlukaniswa nokungcola kwimodeli ebukhali kwaye yaqokelelwa. Emva koko i-LYOPHILY kwaye ihlalutywe yi-HPLC, imveliso ecocekileyo inobunyulu be-98.2% kwaye inokuphinda isetyenziswe ngakumbi kuPhando olulandelayo nophuhliso.
Umzobo 3. I-Clash Chromatogy yesampulu kwikhatriji ye-C18AQ.
Ukuqukumbela, ngokwahlula-hluha c18aq RP ye-carridge edibene nenkqubo ye-flash chromatography yenkqubo yesahlulo™Umatshini unokubonelela ngesisombululo esikhawulezayo nesisebenzayo sokuhlanjululwa kwePolar okanye iisampulu ze-hydrophilic.
Kukho uthotho lwezahlulo ze-P18aq rp carridges eneenkcukacha ezahlukeneyo ezivela kwiTekhnoloji ye-SAntai (njengoko kubonisiwe kwiTheyibhile 2).
| Inombolo yento | Ubungakanani bekholamu | Umgangatho wohambo (ml / min) | Max.pressored (i-psi / bar) |
| SW-5222-004-SP (AQ) | 5.4 g | 5-15 | I-400 / 27.5 |
| SW-5222-012-SP (AQ) | I-20 g | I-10-25 | I-400 / 27.5 |
| SW-5222-025-SP (AQ) | 33 g | I-10-25 | I-400 / 27.5 |
| SW-5222-040-SP (AQ) | 48 g | I-15-30 | I-400 / 27.5 |
| SW-5222-080-SP (AQ) | I-105 g | 25-50 | I-350 / 24.0 |
| SW-5222-120-SP (AQ) | I-155 g | I-30-60 | I-300 / 20.7 |
| SW-5222220-SP (AQ) | I-300 g | 40-80 | I-300 / 20.7 |
| SW-5222-330-SP (AQ) | I-420 g | 40-80 | I-250 / 17.2 |
Itheyibhile 2. Yahlula i-c18aq rp carridges. Izinto zokupakisha: Ukusebenza kakuhle kwe-SP18 (AQ) ye-AICACA, 45 μm, 100 å.
Ngolwazi oluthe kratya malunga neenkcazo ezichaziweyo zomatshini we-Supben ™, okanye ulungelelwaniso lwe-odolo malunga ne-Prefish Carridges, nceda undwendwele iwebhusayithi yethu.
Ixesha leposi: Oct-12 ukuya ku-1218