-
Kuthekani ngokuhambelana kweekholamu zeSepaFlash™ kwezinye iisistim zekhromatografi?
YeSepaFlashTMIikholamu zeStandard Series, izihlanganisi ezisetyenzisiweyo ziyi-Luer-lock in kunye ne-Luer-slip out. Ezi kholamu zinokuxhonywa ngokuthe ngqo kwiinkqubo ze-ISCO's CombiFlash.
Kwi-SepaFlash HP Series, i-Bonded Series okanye i-iLOKTM Series columns, izixhumi ezisetyenzisiweyo ziyi-Luer-lock in kunye ne-Luer-lock out. Ezi kholamu zinokuxhonywa kwiinkqubo ze-ISCO zeCombiFlash kusetyenziswa iiadaptha ezongezelelweyo. Ngeenkcukacha zeziadaptha, nceda ujonge kuxwebhu Santai Adapter Kit for 800g, 1600g, 3kg Flash Columns.
-
Yintoni kanye kanye umthamo wekholamu yoluhlu oludanyazayo?
Umthamo wekholamu yepharamitha (CV) uluncedo ngakumbi ukumisela imiba yokunyusa umgangatho. Ezinye iikhemikhali zicinga ukuba umthamo wangaphakathi wekhatriji (okanye ikholamu) ngaphandle kokupakisha izinto ngaphakathi ngumthamo wekholomu. Nangona kunjalo, umthamo wekholamu engenanto awuyiyo iCV. I-CV yayo nayiphi na ikholam okanye ikhatriji ngumthamo wesithuba esingasetyenziswanga yimathiriyeli epakishwe kwangaphambili kwikholamu. Lo mqulu uquka zombini umthamo we-interstitial (umthamo wendawo ngaphandle kweengqungquthela ezipakishweyo) kunye ne-particle ye-porosity yangaphakathi (i-pore volume).
-
Xa kuthelekiswa ne-silica flash columns, yintoni intsebenzo ekhethekileyo yeekholamu ze-alumina flash?
Iintsika zokukhanya kwe-alumina yenye indlela yokukhetha xa iisampulu zinovakalelo kwaye zithande ukuthotywa kwijeli ye-silica.
-
Uxinzelelo lwangasemva lunjani xa usebenzisa ikholomu edanyazayo?
Uxinzelelo lwangasemva lwekholamu ye-flash inxulumene nobungakanani besuntswana lezinto ezipakishweyo. Izinto ezipakishweyo ezinobungakanani bezinto ezincinci ziya kubangela uxinzelelo oluphezulu lwangasemva kwikholamu ye-flash. Ngoko ke izinga lokuhamba kwesigaba esiphathwayo esisetyenziswa kwikhromatografi yeflash kufuneka ithotywe ngokufanelekileyo ukuze kuthintelwe isixokelelwano sombane ekuyekeni ukusebenza.
Uxinzelelo lwangasemva lwekholamu yokukhanya lukwalingana nobude bekholamu. Umzimba wekholamu omde uya kubangela uxinzelelo oluphezulu lwangasemva kwikholamu edanyazayo. Ngaphezu koko, uxinzelelo lwangasemva lwekholamu ye-flash ihambelana ngokungafaniyo ne-ID (i-diameter yangaphakathi) yomzimba wekholomu. Ekugqibeleni, uxinzelelo lwangasemva lwekholamu ye-flash ilingana ne-viscosity yesigaba esiphathwayo esisetyenziswe kwi-flash chromatography.
