Ikholam yeSepaFlash™

Santai Solvent Lines Kit & Accessories

Hayi, i-silica ene-end-capped ayinyibiliki kuyo nayiphi na i-solvent esetyenziswa ngokuqhelekileyo.

Ukucocwa ngeyona nto iphezulu ngeC18 flash columns, nceda ulandele la manyathelo:
① Gubha ikholamu nge-100% ye-solvent eyomeleleyo (organic) ye-10 - 20 CVs (umthamo wekholomu), ngokuqhelekileyo i-methanol okanye i-acetonitrile.
② Gubha ikholamu nge-50% enamandla + i-50% yamanzi (ukuba izongezo ziyafuneka, zibandakanye) kwenye i-3 - 5 ye-CV.
③ Gubha ikholamu ngeemeko zokuqala zodidi lwe-3 – 5 CVs.

Ubukhulu bekholomu phakathi kwe-4g kunye ne-330g, isinxibelelanisi esiqhelekileyo seLuer sisetyenziswa kule miqolo ye-flash. Kubukhulu bekholamu ye-800g, 1600g kunye ne-3000g, iiadaptha zokudibanisa ezongezelelweyo kufuneka zisetyenziselwe ukunyuswa kwezi kholamu ezinkulu zombane kwi-flash chromatography system. Nceda ujonge uxwebhu Santai Adapter Kit for 800g, 1600g, 3kg Flash Columns ukuze ufumane iinkcukacha ezithe vetshe.

Kwikholamu yesigaba esiqhelekileyo, kucetyiswa ukuba kusetyenziswe isigaba seselula apho umlinganiselo we-methanol ungadluli kwi-25%.

Ngokuqhelekileyo, kucetyiswa ukuba kusetyenziswe isigaba esihambayo apho umlinganiselo we-solvents polar ungadluli kwi-5%. Izinyibilikisi zepolar ziquka iDMSO, iDMF, THF, TEA njl.

Ukulayishwa kwesampulu eqinileyo yindlela eluncedo yokulayisha isampuli ukuba ihlanjululwe kwikholamu, ngakumbi kwiisampuli zokunyibilika okuphantsi. Kule meko, i-ILOK flash cartridge lukhetho olufanelekileyo kakhulu.
Ngokuqhelekileyo, isampuli inyibilika kwi-solvent efanelekileyo kwaye ibhengezwe kwi-adsorbant eqinileyo enokuthi ifane nesetyenziswe kwiikholamu ezikhanyayo, kubandakanywa umhlaba we-diatomaceous okanye i-silica okanye ezinye izinto. Emva kokususwa / ukuphuphuma kwe-solvent eshiyekileyo, i-adsorbent ibekwa phezu kwekholamu ezaliswe ngokuyinxenye okanye kwikhatriji engenanto yokulayisha eqinileyo. Ngolwazi oluthe vetshe, nceda ujonge kuxwebhu iLOK-SL Cartridge User Guide ngeenkcukacha ezithe vetshe.

Umthamo wekholomu uphantse ulingane nomthamo ofileyo (VM) xa ungayihoyi ivolumu eyongezelelweyo kwiityhubhu ezidibanisa ikholamu kunye ne-injector kunye ne-detector.

Ixesha elifileyo (tM) lixesha elifunekayo kuhlaziyo lwecandelo elingagcinwanga.

Umthamo ofileyo (i-VM) ngumthamo wesigaba esiphathwayo esifunekayo ukuze kuveliswe icandelo elingagcinwanga. Umthamo ofileyo ungabalwa ngale equation ilandelayo: VM =F0*tM.

Phakathi kwe-equation engentla, i-F0 yizinga lokuhamba kwesigaba esihambayo.

Hayi, i-silica ene-end-capped ayinyibiliki kuyo nayiphi na i-solvent esetyenziswa ngokuqhelekileyo.

Iikholamu ze-silica flash ziyalahlwa kwaye zisetyenziselwa enye, kodwa ngokuphathwa ngokufanelekileyo, iikhatriji ze-silica zingaphinda zisetyenziswe ngaphandle kokuncama ukusebenza.
Ukuze isetyenziswe kwakhona, ikholomu ye-silica flash kufuneka yomiswe ngokulula ngomoya ocinezelweyo okanye igutyungelwe kwaye igcinwe kwi-isopropanol.

Ugcino olululo luya kuvumela ukuba kuphinde kusetyenziswe iikholamu zeFlash zeC18:
• Ungaze uvumele ikholamu yome emva kokuyisebenzisa.
• Susa zonke izilungisi zendalo ngokugungxula ikholamu nge-80% yemethanol okanye i-acetonitrile emanzini kangange-3 – 5 CVs.
• Gcina ikholamu kwesi sinyibilikisi sokugungxulwa sikhankanywe ngasentla nesifakela isiphelo endaweni.

Kwiikholomu ezinkulu zesayizi ngaphezu kwe-220g, umphumo we-thermal uyabonakala kwinkqubo yokulingana kwangaphambili. Kunconywa ukuseta izinga lokuhamba kwi-50-60% yezinga lokuhamba eliphakanyisiweyo kwinkqubo yokulingana kwangaphambili ukuphepha umphumo ocacileyo we-thermal.

Impembelelo ye-thermal ye-solvent edibeneyo ibonakala ngakumbi kune-solvent enye. Thatha i-solvent system cyclohexane/ethyl acetate njengomzekelo, kucetyiswa ukuba usebenzise i-cyclohexane eyi-100% kwinkqubo yokulingana kwangaphambili. Xa ulungelelwaniso lwangaphambili lugqityiwe, uvavanyo lokwahlula lunokwenziwa ngokwenkqubo yokunyibilikisa esetwe kwangaphambili.