Inkxaso_FAQ BONNER

Umatshini we-wandbean ™

  • Kutheni le nto kufuneka sikwazi ukulinganisa ikholamu ngaphambi kokwahlukana?

    Ukulungelelaniswa kwekholamu kungayikhusela ikholamu ukuba ingonakaliswa sisiphumo sokukhwela xa isolvent sifula ngokukhawuleza kwikholamu. Ngelixa i-silica iyoyileyo yangaphambi kokuba ifakwe kwikholamu inxibelelana neSolvent okokuqala ngexesha lokwahlukana, ubushushu obuninzi bunokukhululwa ngakumbi xa i-solvent iqhuma kwinqanaba lokuhamba okuphezulu. Olu bushushu lunokubangela umzimba wekholamu ukuba usebenze kwaye ke ngoko ke ugcino oluvela kwikholamu. Ngamanye amaxesha, obu bushushu bunokuyonakalisa isampulu enobushushu.

  • Ungayenza njani xa ipump ivakale kakhulu kunangaphambili?

    Inokubangelwa kukungabikho kweoyile ethambisa ioyile kwishayile ejikelezayo yempompo.

  • Nguwuphi umthamo weetyhefu kunye nonxibelelwano ngaphakathi kwesixhobo?

    Umthamo opheleleyo we-tubing yenkqubo, isinxibelelanisi kunye negumbi lokudibanisa limalunga ne-25 ml.

  • Ungayenza njani xa uphendula ngendlela engalunganga kwi-flash chromatom, okanye ipinki ye-Elking kwi-flash chromatom ye-chromatogram ingenguye ...

    Iseli yokuhamba kwemodyuli yokufuma ingcoliseka yisampulu enolwazi oluqinileyo lwe-UV. Okanye kunokubangelwa kukucombulula i-uv Nceda wenze le ngqesho ilandelayo:

    1. Susa ikholamu yeFlash kwaye ugungxule inkqubo ityhubhu ene-polar ye-polar ilandelwa yi-Solver ebuthathaka.

    I-2. Songa iNgxaki ye-Ackerve: Umzekelo ngelixa i-N-Hexane kunye neDichloromethane (i-DCM) iqeshwa i-SCRM kwi-DCM kwi-254 NM iphantsi kune-N-Hexane. Kwimeko yento eyenzekayo, singakwazi ukuyiphatha ngokucofa iqhosha "zero" kwi-Supriction Page ewahlelayo.

    3. Iseli yokuhamba kwemodyuli yokufumanisa ingcolisekile kwaye kufuneka icocwe icocekile.

  • Ungayenza njani xa intloko yabangamkizi ayiphakamisi ngokuzenzekelayo?

    Inokubangelwa kukuba uqhagamshelo kwintloko yekholamu kwaye kwinxalenye yesiseko ityhutywe ngokwenza isinyibilikisi ukuze izivakalisi zibambekile.

    Umsebenzisi unokunyusa ngesandla intloko yekholamu ngokusebenzisa amandla amancinci. Xa intloko yabangabambanga iKholamu iphakanyiselwe ekuphakameni okuthile, intloko yabangamkizi kufuneka ikwazi ukushukunyiswa ngokuchukumisa amaqhosha kuyo. Ukuba intloko yekholamu ayinakuphakanyiswa ngesandla, umsebenzisi kufuneka anxibelelane nenkxaso yezobugcisa yendawo.

    Enye indlela yongxamiseko: Umsebenzisi unokufaka ikholamu ngaphezulu kwentloko yekholamu endaweni yoko. Isampulu yereyitha inokufakwa ngqo kwikholamu. Isampulu yesampulu eqinileyo inokufakwa ngaphezulu kwekholam yokwahlulahlula.

  • Ungayenza njani into yokuba ubukhulu bomthombo bubuthathaka?

    1. Amandla asezantsi omthombo wokukhanya;

    2. Iphuli yedini ingcolile; I-ITUATRE, akukho cala libonakalayo okanye i-Peak ebonakalayo incinci ngokwahlula-hlulwa, amandla abonakalayo abonisa ixabiso elingaphantsi kwe-25%.

    Nceda ugungxule ityhubhu enesimo esifanelekileyo kwi-10ml / min ye-30min kwaye ugcine i-spectrium.if akukho tshintsho kumthombo wokukhanya, nceda ubeke endaweni ye-Deuterium Lamp; Ukuba i-spectrum itshintshiwe, ichibi lesasazwe lingcolile, nceda uqhubeke nokucoca ngesifo esifanelekileyo.

  • Ungayenza njani xa umatshini okuvuza ngaphakathi?

    Nceda ujonge ityhubhu kunye nomnxibelelanisi rhoqo.

  • Ungayenza njani into yokuba isiseko sihlala siye phezulu xa i-Ethyl Acetate yayiqeshwe njengezisombulisi?

    Ukufunyanwa kwabekwe kwi-wavlengd kwi-wavnength engaphantsi kwe-245 nts ukuba i-ithyl acetate inolwazi olubonakalayo kwi-245nm. Ukuhamba kwesiseko kuya kuba yinto enkulu xa i-ethyl acetate isetyenziswa njengeSolvent ye-Elvent kwaye sikhetha i-220 NM njengoko ifunyanwe i-waveength yavelen.

    Nceda utshintshe ukufumanisa i-wavelength. Kucetyiswa ukukhetha i-254nm njengoko ifumene i-wavevength. Ukuba i-220 NM kuphela kwe-wavelength kuphela yokufunyanwa kwesampulu, umsebenzisi kufuneka aqokelele i-auent onesifo sokugweba kunye ne-Solvent egqithisileyo inokuqokelelwa kule meko.

  • Ungayenza njani xa iibhubhu zifumaneka kwikholamu yangaphambi kwekholamu?

    Coca intloko yentloko ye-Solvent ngokupheleleyo ukususa nayiphi na impatho. Sebenzisa i-ethanol okanye i-isopropanol yokuqhubela phambili le nkqubo ngokupheleleyo ukuphepha iingxaki zokuzila izilwanyana.

    Ukucoca intloko ye-Solvent, ukonakalisa icebo lokucoca kwintloko yecebo lokucoca kwaye ucoce ngebrashi encinci. Emva koko hlamba icebo lokucoca ne-ethanol kwaye lome ngayo. Faka kwakhona intloko yecebo lokusebenzisa elizayo.

  • Ungatshintsha njani phakathi kokwahlukana kwesigaba esiqhelekileyo kunye nokwahlulahlula iSigaba seSigaba?

    Nokuba ukutshintshela ukusuka ekwinqanaba lesigaba esiqhelekileyo lokwahlulahlula-hlulwa kwakhona okanye kwi-Ethanol okanye i-Isopropanol kufuneka isetyenziswe njengesinyibilikisi sokutshisa ngokupheleleyo.

    Kucetyiswa ukuseta ireyithi yokuhamba nge-40 ml / min ukuze igungxule imigca yeSolvent kunye nazo zonke iityhubhu zangaphakathi.

  • Ungayenza njani xa umnini wekholamu engakwazi ukudityaniswa kunye nezantsi komnini wekholamu ngokupheleleyo?

    Nceda uphinde uphinde uphinde ufake amazantsi omnini wekholamu emva kokukhulula isikere.

  • Ungayenza njani ukuba uxinzelelo lwenkqubo jika liphezulu kakhulu?

    I-1. Inkqubo yokuhamba kwenkqubo iphezulu kakhulu kwikholam yeflash yangoku.

    2. Isampulu inomhlaba ombi kunye nokungabinamthi kwiSigaba seSoftware, ngaloo ndlela sikhokelela kwi-bloung block.

    I-3. Esinye isizathu sibangela ukuba i-bloung block.