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Ukwenza njani xa amaqamza efunyenwe kwityhubhu yangaphambili yekholomu?
Coca intloko yokucoca isinyibilikisi ngokupheleleyo ukususa nakuphi na ukungcola. Sebenzisa i-ethanol okanye i-isopropanol ukugungxula inkqubo ngokupheleleyo ukuze ugweme iingxaki ze-solvent ezingenakulinganiswa.
Ukucoca intloko yokucoca i-solvent, qhawula isihluzo kwintloko yokucoca kwaye uyihlambulule nge-brush encinci. Emva koko hlamba isihluzo nge-ethanol kwaye usomise. Hlanganisa kwakhona intloko yokucoca ukuze uyisebenzise kwixesha elizayo.
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Indlela yokutshintsha phakathi kokuhlukana kwesigaba esiqhelekileyo kunye nokwahlukana kwesigaba esibuyiselwe umva?
Nokuba utshintshe ukusuka kwisigaba esiqhelekileyo sokwahlulwa ukuya kukwahlulwa kwesigaba esibuyiselwe umva okanye ngokuchaseneyo, i-ethanol okanye i-isopropanol kufuneka isetyenziswe njengesinyibilikisi sotshintsho ukugungxula ngokupheleleyo naziphi na izinyibilikisi ezingaxutywanga kwi-tubing.
Kucetyiswa ukuba usete izinga lokuhamba kwi-40 mL / min ukucima imigca ye-solvent kunye nazo zonke iibhubhu zangaphakathi.
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Indlela yokwenza xa umnini wekholomu engakwazi ukudibaniswa kunye nezantsi Umphathi weKholam ngokupheleleyo ?
Nceda ubeke umphantsi wesibambi sekholamu emva kokukhulula isikrufu.
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Ukwenza njani ukuba uxinzelelo lwenkqubo lujika kakhulu?
1. Izinga lokuqukuqela kwesixokelelwano liphezulu kakhulu kwikholamu yomenyezi yangoku.
2. Iisampulu ine-solubility engalunganga kunye ne-precipitates ukusuka kwisigaba esihambayo, oko kubangele ukuvaleka kweetyhubhu.
3. Esinye isizathu sibangela ukuvaleka kweetyhubhu.
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Ungenza njani xa isibambi somhlathi sinyuka sisehla ngokuzenzekelayo emva kokuqalisa ?
Ubume bendawo bumanzi kakhulu, okanye ukuvuza kwe-solvent ukuya ngaphakathi kwesibambi sekholomu kubangela isiphaluka esifutshane. Nceda ufudumeze isibambi sekholamu ngokufanelekileyo ngesomisi seenwele okanye ngompu womoya oshushu emva kokucima kombane.
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Indlela yokwenza xa i-solvent ifunyenwe ivuza kwisiseko somphathi wekholomu xa umnini wekholomu ephakamisa?
Ukuvuza kwe-solvent kunokuba ngenxa yenqanaba lokunyibilikisa kwibhotile yenkunkuma liphezulu kunobude bomdibaniso kwisiseko somphathi wekholamu.
Beka ibhotile yenkunkuma phantsi kweqonga lokusebenza lesixhobo, okanye ukhawuleze uhlehlise umnini wekholomu emva kokususa ikholamu.
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Yintoni umsebenzi wokucoca kwi "Pre-separation"? Ngaba kufuneka yenziwe?
Lo msebenzi wokucoca wenzelwe ukucoca umbhobho wenkqubo phambi kokuba kuqhutywe ukwahlukana. Ukuba "ukucoca emva kokucoca" kuye kwenziwa emva kokuphela kokwahlukana, eli nyathelo lingatsitywa. Ukuba ayenziwanga, kucetyiswa ukuba wenze le nyathelo lokucoca njengoko kuyalelwe yinkqubo ye-prompt.
