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Unokwenza njani xa unomathotholo we-solvents ungachanekanga?
Coca intloko yokucoca i-solvent ngokupheleleyo ukususa nayiphi na into engcolileyo, Kungcono ukusebenzisa ukucocwa kwe-ultrasonic.
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Yintoni ebangela ingxolo ephezulu yesiseko ?
1. I-flow cell ye-detector yangcoliswa.
2. Amandla aphantsi omthombo wokukhanya.
3. Impembelelo yempompo ye-pulse.
4. Ubushushu besiphumo se-detector.
5. Kukho amaqamza kwidama lovavanyo.
6. Ikholomu okanye ukungcoliseka kwesigaba esihambayo.
Kwichromatography elungiselelayo, inani elincinci lengxolo yesiseko linefuthe elincinci ekwahlukaneni.
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Ungenza njani ukuba i-alam yenqanaba lolwelo ngokungaqhelekanga?
1. Umdibaniso wombhobho ngasemva komatshini ukhululekile okanye wonakaliswe; Buyisela indawo yokudibanisa ityhubhu;
2. Ivalve yokukhangela indlela yegesi yonakele. Buyisela ivalve yokutshekisha.
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Ungenza njani ukuba ingxelo yembali iyakhuthaza
Emva kokuhlukana, kuyimfuneko ukulinda imizuzu emi-3-5 ngaphambi kokuba uvale ukuze uqinisekise ukuthembeka kweerekhodi zovavanyo.
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Kutheni kufuneka silinganise ikholomu ngaphambi kokuhlukana?
Ulungelelwaniso lwekholomu lunokukhusela ikholam ekubeni yonakaliswe yimpembelelo ye-exothermic xa i-solvent ikhupha ngokukhawuleza kwikholamu. Ngelixa i-silica eyomileyo ifakwe ngaphambili kwikholomu idityaniswe ne-solvent okokuqala ngexesha lokuhlukana, ubushushu obuninzi bunokukhutshwa ngokukodwa xa i-solvent iguqa kwizinga eliphezulu lokuhamba. Obu bushushu bunokubangela ukuba umzimba wentsika ube wonakaliswe kwaye ngaloo ndlela uvuza okunyibilikayo kwikholamu. Kwezinye iimeko, obu bushushu bunokonakalisa isampulu ebuthathaka kubushushu.
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Ungenza njani xa impompo ivakala kakhulu kunangaphambili?
Mhlawumbi kubangelwa ukungabikho kweoli yokuthambisa kwi-shaft ejikelezayo yempompo.
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Uthini umthamo wetyhubhu kunye noqhagamshelo ngaphakathi kwesixhobo?
Umthamo opheleleyo we-tubing yenkqubo, i-connetors kunye negumbi lokuxuba malunga ne-25 mL.
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Kwenziwa njani xa umqondiso ongalunganga wempendulo kwichromatogram edanyazayo, okanye incopho yekhromatogram edanyazayo ingaqhelekanga...
Iseli yokuhamba yemodyuli ye-detector ingcolisekile yisampuli ene-UV eyomeleleyo yokufunxa. Okanye inokuba kungenxa yokufunxa kwe-solvent ye-UV eyinto eqhelekileyo. Nceda wenze lo msebenzi ulandelayo:
1. Susa ikholomu edanyazayo kwaye ugungxule iityhubhu yesixokelelwano ngesinyibilikisi esinamandla sepolar emva koko silandelwe sisinyibilikisi esibuthathaka esikwipolar.
2. Ukunyibilika kwengxaki yokufunxa kwe-UV: umz. ngelixa i-n-hexane kunye ne-dichloromethane (DCM) ziqeshwe njenge-solvent eluting, njengoko umlinganiselo we-DCM usanda, isiseko sechromatogram sinokuqhubeka ukuba ngaphantsi kwe-zero kwi-Y-axis ukususela ekufunjweni kwe-DCM. kwi-254 nm ingaphantsi kunaleyo ye-n-hexane. Kwimeko yale nto yenzekayo, sinokuyibamba ngokucofa iqhosha elithi "Zero" kwiphepha eliqhubayo lokwahlula kwiSepaBean App.
3.I-flow cell yemodyuli ye-detector ingcoliseke kakhulu kwaye ifuna ukucocwa nge-ultrasonically.
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Indlela yokwenza xa intloko yomphathi wekholomu ingaphakamisi ngokuzenzekelayo ?
Isenokuba ngenxa yokuba izihlanganisi kwintloko yesibambi sekholamu ngokunjalo nakwisiseko sidumbile yi-solvent ukuze izihlanganisi zixinge.
Umsebenzisi unokuphakamisa ngesandla intloko yomphathi wekholamu ngokusebenzisa amandla amancinci. Xa intloko yomphathi wekholomu iphakanyiselwe kumphakamo othile, intloko yomphathi wekholamu kufuneka ikwazi ukushukunyiswa ngokuchukumisa amaqhosha aphezu kwayo. Ukuba intloko yomphathi wekholomu ayinakuphakanyiswa ngesandla, umsebenzisi kufuneka aqhagamshelane nenkxaso yobugcisa bendawo.
Enye indlela kaxakeka: Umsebenzisi unokufakela ikholamu phezulu kwentloko yesibambi sekholamu endaweni yoko. Isampuli yolwelo inokutofwa ngokuthe ngqo kwikholamu. Ikholomu yokulayisha isampula eqinile ingafakwa phezulu kwikholamu yokwahlula.
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Ungenza njani ukuba ubunzulu besixhobo buba buthathaka?
1. Amandla aphantsi omthombo wokukhanya;
2. Idama lokujikeleza lingcolisekile; I-intuitively, akukho ngqungquthela ye-spectral okanye i-spectral peak incinci ukuhlukana , I-spectra yamandla ibonisa ixabiso elingaphantsi kwe-25%.
Nceda uhlambe ityhubhu nge-solvent efanelekileyo kwi-10ml / min ye-30min kwaye uqaphele i-spectrum yamandla.Ukuba akukho tshintsho kwi-spectrum, kubonakala ngathi amandla aphantsi omthombo wokukhanya, nceda ubeke isibane se-deuterium; Ukuba i-spectrum itshintshile, idama lokujikeleza lingcolisekile, nceda uqhubeke nokucoca ngesinyibilikisi esifanelekileyo.
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Ungenza njani xa umatshini uvuza ulwelo ngaphakathi?
Nceda ujonge ityhubhu kunye nesinxibelelanisi rhoqo.
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Kwenziwa njani ukuba isiseko sihlala sinyukela phezulu xa i-ethyl acetate yayisetyenziswa njengesinyibilikisi esiluting?
Ubude be-wavelength bokubona bubekwe kwi-wavlength engaphantsi kwe-245 nm ukususela ekubeni i-ethyl acetate ine-absorption eyomeleleyo kuluhlu lokufumanisa olungaphantsi kwe-245nm. Isiseko sokukhukuliseka siya kuba sisona sibalaseleyo xa i-ethyl acetate isetyenziswa njenge-eluting solvent kwaye sikhetha i-220 nm njengobude bokubona ubude.
Nceda utshintshe ubude bewavenge. Kuyacetyiswa ukuba ukhethe i-254nm njengobude bokubona ubude. Ukuba i-220 nm kuphela kobude obulungele ukuchongwa kwesampulu, umsebenzisi kufuneka aqokelele i-eluent ngokugweba ngononophelo kwaye isinyibilikisi esigqithisileyo sinokuqokelelwa kulo mzekelo.
