I-FAQS

Coca intloko yentloko yeSolvent ngokupheleleyo ukuze ususe nayiphi na impatho, kungcono ukusebenzisa ukucoca i-ultrasonic.

1. Iseli yokuhamba komcuphi wangcoliswa.

2. Amandla asezantsi omthombo wokukhanya.

I-3. Impembelelo yempompo yempompo.

4. Ifuthe lobushushu be-cittocki.

5. Kukho amaqamza kwichibi lovavanyo.

Ikholamu okanye isiseko senqanaba leSigaba seSoftware.

Kwi-chromatography yokulungiselela, isixa esincinci sengxolo esisisiseko asinampembelelo encinci yokwahlukana.

1. Isinxibelelanisi se-tube ngasemva komatshini sikhululekile okanye sonakalisiwe; Buyisela isinxibelelanisi seTube;

I-2. Indlela yegesi yegesi yonakele. Faka endaweni yeValve yokujonga.

Emva kokwahluka, kuyimfuneko ukulinda imizuzu emi-3-5 ngaphambi kokuvala ukuqinisekisa ukuthembeka kweerekhodi zokuvavanya.

Ukulungelelaniswa kwekholamu kungayikhusela ikholamu ukuba ingonakaliswa sisiphumo sokukhwela xa isolvent sifula ngokukhawuleza kwikholamu. Ngelixa i-silica iyoyileyo yangaphambi kokuba ifakwe kwikholamu inxibelelana neSolvent okokuqala ngexesha lokwahlukana, ubushushu obuninzi bunokukhululwa ngakumbi xa i-solvent iqhuma kwinqanaba lokuhamba okuphezulu. Olu bushushu lunokubangela umzimba wekholamu ukuba usebenze kwaye ke ngoko ke ugcino oluvela kwikholamu. Ngamanye amaxesha, obu bushushu bunokuyonakalisa isampulu enobushushu.

Inokubangelwa kukungabikho kweoyile ethambisa ioyile kwishayile ejikelezayo yempompo.

Umthamo opheleleyo we-tubing yenkqubo, isinxibelelanisi kunye negumbi lokudibanisa limalunga ne-25 ml.

Iseli yokuhamba kwemodyuli yokufuma ingcoliseka yisampulu enolwazi oluqinileyo lwe-UV. Okanye kunokubangelwa kukucombulula i-uv Nceda wenze le ngqesho ilandelayo:

1. Susa ikholamu yeFlash kwaye ugungxule inkqubo ityhubhu ene-polar ye-polar ilandelwa yi-Solver ebuthathaka.

I-2. Songa iNgxaki ye-Ackerve: Umzekelo ngelixa i-N-Hexane kunye neDichloromethane (i-DCM) iqeshwa i-SCRM kwi-DCM kwi-254 NM iphantsi kune-N-Hexane. Kwimeko yento eyenzekayo, singakwazi ukuyiphatha ngokucofa iqhosha "zero" kwi-Supriction Page ewahlelayo.

3. Iseli yokuhamba kwemodyuli yokufumanisa ingcolisekile kwaye kufuneka icocwe icocekile.

Inokubangelwa kukuba uqhagamshelo kwintloko yekholamu kwaye kwinxalenye yesiseko ityhutywe ngokwenza isinyibilikisi ukuze izivakalisi zibambekile.

Umsebenzisi unokunyusa ngesandla intloko yekholamu ngokusebenzisa amandla amancinci. Xa intloko yabangabambanga iKholamu iphakanyiselwe ekuphakameni okuthile, intloko yabangamkizi kufuneka ikwazi ukushukunyiswa ngokuchukumisa amaqhosha kuyo. Ukuba intloko yekholamu ayinakuphakanyiswa ngesandla, umsebenzisi kufuneka anxibelelane nenkxaso yezobugcisa yendawo.

Enye indlela yongxamiseko: Umsebenzisi unokufaka ikholamu ngaphezulu kwentloko yekholamu endaweni yoko. Isampulu yereyitha inokufakwa ngqo kwikholamu. Isampulu yesampulu eqinileyo inokufakwa ngaphezulu kwekholam yokwahlulahlula.

1. Amandla asezantsi omthombo wokukhanya;

2. Iphuli yedini ingcolile; I-ITUATRE, akukho cala libonakalayo okanye i-Peak ebonakalayo incinci ngokwahlula-hlulwa, amandla abonakalayo abonisa ixabiso elingaphantsi kwe-25%.

Nceda ugungxule ityhubhu enesimo esifanelekileyo kwi-10ml / min ye-30min kwaye ugcine i-spectrium.if akukho tshintsho kumthombo wokukhanya, nceda ubeke endaweni ye-Deuterium Lamp; Ukuba i-spectrum itshintshiwe, ichibi lesasazwe lingcolile, nceda uqhubeke nokucoca ngesifo esifanelekileyo.

Nceda ujonge ityhubhu kunye nomnxibelelanisi rhoqo.

Ukufunyanwa kwabekwe kwi-wavlengd kwi-wavnength engaphantsi kwe-245 nts ukuba i-ithyl acetate inolwazi olubonakalayo kwi-245nm. Ukuhamba kwesiseko kuya kuba yinto enkulu xa i-ethyl acetate isetyenziswa njengeSolvent ye-Elvent kwaye sikhetha i-220 NM njengoko ifunyanwe i-waveength yavelen.

Nceda utshintshe ukufumanisa i-wavelength. Kucetyiswa ukukhetha i-254nm njengoko ifumene i-wavevength. Ukuba i-220 NM kuphela kwe-wavelength kuphela yokufunyanwa kwesampulu, umsebenzisi kufuneka aqokelele i-auent onesifo sokugweba kunye ne-Solvent egqithisileyo inokuqokelelwa kule meko.