Mingzu Yang, Bo Xu
Codsiga R & D Center
Gogoldhig
Antibiyootigyadu waa fasal ka mid ah dheefaha dheecaanka sare ee ay soo saareen shebaarka labaad ee loo yaqaan '' '' '' Antibiyootigyadu waxay carqaladeyn karaan koritaanka iyo jiritaanka sheyga kale ee microrgarinisms. Antibiyootihii ugu horreeyay ee uu helay bini-aadamka, penicillin, waxaa soo ogaaday in bylobixiraha Ingiriiska ah 1928. Wuxuu arkay in bakteeriyada ku jirta agagaarka caaryada aan ku dhex dhicin xargaha dhaqanka ee muusikada. Wuxuu dhajiyay in caaryada ay tahay inay qarsato walax bakteeriyada ka hortaga bakteeriyada, kaas oo uu u magacaabay Penicillin sanadkii 1928. Si kastaba ha noqotee, maaddooyinka firfircoon lama daahin waqtigaas. Sanadkii 1939, silsiladda Ernst iyo Howard Fley of Oxford University Jaamacadda Oxford ayaa go'aansatay inay horumariso dawo daweyn karta infekshinka bakteeriyada. Ka dib markii aad la xiriireyso qulqulka si aad u hesho noocyo, waxay si guul leh u soo saareen oo loo nadiifiyey cirbadaha. Horumarka ugu guulaha badan ee penicillin ee daroogada daweynta, qulqulka, silsiladda iyo caloosha waxay la wadaageen abaalmarinta 'Nobel Prize'.
Antibiyootigyada waxaa loo isticmaalaa sida wakiilo ka hortaga bakteeriyada si loo daweeyo ama looga hortago infekshinka bakteeriyada. Waxaa jira dhowr nooc oo waaweyn oo antibiyootig ah oo loo isticmaalo walxaha bakteeriyada ka hortagga ah: halsano, synthesis iyo wadarta isku-darka. Antibiyootigyada ay soo saartay halsano bayooloji ah ayaa loo baahan yahay in si habsami leh loogu habeeyo habab kiimiko ah oo ay sabab u tahay xasilloonida kiimikada awgeed, waxyeellooyinka sunta ah, muuqaalka bakteeriyada bakteeriyada ka hortaga iyo arrimo kale oo bakteeriyada ka hortaga ah. Ka dib markii kimikarku wax laga beddelay, antibiyootigyadu waxay gaari karaan xasillooni dheeri ah, waxyeellooyinka muuqaalka sunta ah, oo la ballaariyey bakteeriyada bakteeriyada bakteeriyada, oo la hagaajiyay caabbinta dawooyinka ka hortaga ah, oo ay wanaajiso saamaynta daaweynta daroogada. Sidaa darteed, antibiyootigga semibiyootigyada ee semibiotics hadda waa jihada ugu caansan horumarinta dawooyinka antibiyootigga.
Kobcinta antibiyootigga semi-sen-sent-sence-ka, antibaayootikku waxay leeyihiin astaamaha xoqida hooseeya, badeecadaha badan iyo qaybo murugsan maadaama laga soo qaatay wax soo saarka halsano ee microbial. Xaaladdan oo kale, falanqaynta iyo xakamaynta ciladaha ee antibiyootiga dhimirka ee antibiyootiga ah ayaa si gaar ah muhiim u ah. Si si wax ku ool ah loo ogaado oo lagu garto ciladaha, waxaa lagama maarmaan ah in la helo lacag ku filan oo ah wax soo saarka wax soo saarka shey isku-darka ah ee antibiyootiga dhimirka. Waxaa ka mid ah farsamooyinka wax-ka-abuurka ee caadiga ah, Toomalka Toomalka ah waa hab qaali ah oo leh faa'iidooyin sida qaddarka muunad weyn, qiimaha waqtiga, iwm.
Qoraalkan, wasakhda ugu weyn ee antibiyootigga antibiyootigga ee antibiyootigga ee aminagllycoside-ka ee Aminogycoside ayaa loo adeegsaday muunadda oo loo daahirinayo santuuqa sepaflash C18AQ Chrotridge chpabean ™ servicean ™ servicean ™ servicean ™ servicean ™ servicean. Shirka bartilmaameedka la bartilmaameedsaday Shuruuda ayaa si guul leh loo helay, oo u soo jeedinaya xallin aad u hufan oo ku habboon nadiifinta xeryahooda.
Qaybta tijaabada
Muunadda waxaa si naxariis leh u siiyay shirkad dawooyin maxalli ah. Muunadda waxay ahayd nooc ka mid ah karbohaydraycyada amino polycychic-ka ah waxaana qaab dhismeedka molilal uu la mid yahay antibiyootig antibiyootig antinoglycoside. Farsamooyinka muunadda waxay ahayd halkii aad ka sarreysay, taasoo aad u ka dhigeysa biyo. Jaantusyada qaab dhismeedka qaab dhismeedka molokalka ee muunadda ayaa lagu muujiyey Jaantuska 1. Daahirnimada muunadda ceeriin waxay ahayd 88% sida lagu falanqaynin hplc. Daahirinta xeryahooda ku saabsan polarity-ka sare, muunad ayaa loo isticmaali doonaa sadarka caadiga ah ee C18 si waafaqsan waaya-aragnimadeena hore. Sidaa darteed, sadarka C18AQ wuxuu u shaqeeyey muunadda muunadda.
Jaantus 1. Jaantusyada qorshaynta qaab dhismeedka muunadda ee muunadda.
Si loo diyaariyo tusaalaha tusaalaha, muunad mg ceyriin ah ayaa lagu kala diray 5 ml biyo saafi ah ka dibna waxaa lagu kala diray si uu u sameeyo si uu u noqdo xalka gabi ahaanba cad. Xalka tusaalaha ayaa markaa lagu muday safka tooshka ah ee dushiisa. Dejinta tijaabada ee daahirinta Flash ayaa lagu taxay shaxda 1.
| Qalab | Mashiinka Sepabisean ™ 2 | |
| Cartroid | 12 g sepaflash c18aq rp flash shuttridge (spersical), 20 - 45μm, 100 Å 52222-012-sp (AQ)) | |
| Shaab maamuus | 204 nm, 220 nm | |
| Wajiga mobilada | KOOXDA A: Biyo Solvent B: acetonitrile | |
| Sicirka qulqulka | 15 ml / min | |
| Muunad qaadida | 50 mg | |
| Heer deg-degoobid | Waqtiga (min) | XULASHADA B (%) |
| 0 | 0 | |
| 19.0 | 8 | |
| 47.0 | 80 | |
| 52.0 | 80 | |
Natiijooyinka iyo wada hadalka
Tooshka Toomaalka ee muunadda ee muunad C18Aq ee ka soo muuqday Jaantuska 2. Sida ka muuqata Jaantuska 2, muunadda weyn ee loo yaqaan 'polar' ayaa si wax ku ool ah loogu hayey C18Aq Chtridge. Ka dib markii Lyaphonization ee jajabyada la soo ururiyey, sheyga bartilmaameedka ah wuxuu daahirsanaaday 96.2% (sida ka muuqata Jaantuska 3) by falanqaynta HPLC. Natiijooyinka ayaa tilmaamay in badeecada la daahiriyey laga faafin karo cilmi baarista iyo horumarka.
Jaantus 2. Tooshka Tooshka Flash ee muunadda ee muunad C18Aq Chtridge.
Jaantus 3. Chryomanka HPLCC ee sheyga bartilmaameedka ah.
In conclusion, SepaFlash C18AQ RP flash cartridge combined with the flash chromatography system SepaBean™ machine could offer a fast and effective solution for the purification of highly polar samples.
Ku saabsan 'sepaflash c18aq rp rp flash cartridedings'
Waxaa jira taxane ah oo ah sepeplash c18aq c18aq rp flafder kartoonnada iyada oo sharraxaad ah oo kala duwan oo ka socda tikniyoolajiyadda Statai (sida lagu muujiyey Shaxda 2).
| Lambarka Shayga | Cabbirka sadarka | Sicirka qulqulka (ml / min) | Max.pressure (Psi / bar) |
| SW-5222-004-sp (AQ) | 5.4 g | 5-15 | 400 / 27.5 |
| SW-5222-012-Sp (AQ) | 20 g | 10-25 | 400 / 27.5 |
| Sw-5222-025-sp (AQ) | 33 g | 10-25 | 400 / 27.5 |
| SW-5222-040-SP (AQ) | 48 g | 15-30 | 400 / 27.5 |
| SW-5222-080-SP (AQ) | 105 g | 25-50 | 350 / 24.0 |
| SW-5222-120-sp (AQ) | 155 g | 30-60 | 300 / 20.7 |
| SW-5222-220-sp (AQ) | 300 g | 40-80 | 300 / 20.7 |
| SW-5222-330-SP (AQ) | 420 g | 40-80 | 250 / 17.2 |
Jadwalka 2aad Qalabka xirxirayaasha
Macluumaad dheeri ah oo ku saabsan faahfaahinta faahfaahinta ee mashiinka sepabeanean ™, ama macluumaadka dalabka ee taxanaha sepaflash-ka Flash-ka Flash Flash-ka, fadlan booqo websaydhka.
Waqtiga Post: Oct-26-2018