Mingzu Yang, Bo Xu
Codsiga Xarunta R&D
Hordhac
Antibiyootigyadu waa qayb ka mid ah dheef-shiid kiimikaad sare oo ay soo saaraan noolaha (oo ay ku jiraan bakteeriyada, fangaska, actinomycetes) ama xeryahooda la mid ah kuwaas oo kiimiko ahaan la sameeyay ama qayb-samaysan.Antibiyootigyadu waxay hor istaagi karaan koritaanka iyo badbaadada noolaha kale.Antibiyootiggii ugu horreeyay ee uu helo bini'aadamka, penicillin, waxaa daahfuray microbiologist Ingiriis Alexander Fleming 1928. Wuxuu arkay in bakteeriyada agagaarka caaryada aysan ka bixi karin saxanka dhaqanka staphylococcus kaas oo wasakhaysan caaryada.Waxa uu dhajiyay in caaryada ay tahay inay soo saarto walaxda bakteeriyada, oo uu u magacaabay penicillin 1928. Si kastaba ha ahaatee, maaddooyinka firfircoon lama nadiifin wakhtigaas.Sannadkii 1939kii, Ernst Chain iyo Howard Florey oo ka tirsan jaamacadda Oxford waxay go'aansadeen inay soo saaraan dawo daweyn karta caabuqyada bakteeriyada.Ka dib markii ay la xiriireen Fleming si ay u helaan nuucyo, waxay si guul leh uga soo saareen oo ay nadiifiyeen penicillin ka nuucyada.Horumarkooda guusha leh ee penicillin oo ah dawo daweyn, Fleming, Chain iyo Florey waxay wadaageen 1945 Nobel Prize ee Daawada.
Antibiyootigyada waxaa loo isticmaalaa sidii bakteeriyada bakteeriyada si loo daweeyo ama looga hortago caabuqyada bakteeriyada.Waxaa jira dhowr qaybood oo waaweyn oo antibiyootiko ah oo loo isticmaalo sida bakteeriyada bakteeriyada: β-lactam antibiyootiko (oo ay ku jiraan penicillin, cephalosporin, iwm.), Antibiyootikada aminoglycoside, antibiyootiga macrolide, antibiotics tetracycline, chloramphenicol (wadarta antibiyootiga synthetic), iyo iwm. Ilaha antibiyootiga waxaa ka mid ah. halsano nafleyda, semi-synthesis iyo wadarta guud.Antibiyootigyada ay soo saarto halsano bayooloji waxay u baahan tahay in qaab dhismeed lagu beddelo hababka kiimikada iyadoo ay ugu wacan tahay xasilloonida kiimikada, waxyeellooyin sun ah, spectrum bakteeriyada iyo arrimo kale.Ka dib markii si kiimiko ah wax laga beddelo, antibiyootiga waxay gaari karaan xasillooni korodhay, hoos u dhac ku yimaada saamaynta sunta ah, balaadhinta bakteeriyada bakteeriyada, iska caabbinta daawada oo yaraada, helitaanka noolaha oo wanaajisay, oo markaas ay hagaajinayso saamaynta daawaynta.Sidaa darteed, antibiyootiga semi-synthetic ayaa hadda ah jihada ugu caansan ee horumarinta daawooyinka antibiyootiga.
Horumarinta antibiyootigyada semi-synthetic, antibiyootiga waxay leeyihiin sifooyinka nadiifnimada hooseeya, waxyaabo badan oo ka mid ah alaabta iyo qaybaha adag tan iyo markii ay ka soo jeedaan alaabta halsano ee microbial.Xaaladdan oo kale, falanqaynta iyo xakamaynta wasakhda ee antibiyootiga semi-synthetic ayaa si gaar ah muhiim u ah.Si si wax ku ool ah loo aqoonsado oo loo garto wasakhda, waxaa lagama maarmaan ah in la helo qadar ku filan oo wasakh ah oo laga helo alaabta synthetic ee antibiyootiga semi-synthetic.Farsamooyinka diyaarinta wasakhda ee inta badan la isticmaalo, chromatography flash waa hab wax ku ool ah oo leh faa'iidooyin ay ka mid yihiin muunado badan oo la raray, kharash yar, waqti badbaadin, iwm.
Boostada dhexdeeda, wasakhda ugu weyn ee antibiyootiga semi-synthetic aminoglycoside antibiyootiga waxaa loo adeegsaday muunad ahaan waxaana lagu sifeeyay kartridge SepaFlash C18AQ oo ay weheliso nidaamka chromatography-ga ee SepaBean ™ mashiinka.Shayga la beegsanayo ee buuxinaya shuruudaha ayaa si guul leh loo helay, isagoo soo jeedinaya xal aad u hufan oo lagu nadiifiyo xeryahooda.
Qaybta Tijaabada
Muunadda waxaa si naxariis leh u bixisay shirkad dawooyin ah oo maxalli ah.Muunadu waxay ahayd nooc ka mid ah karbohaydraytyada polycyclic amino iyo qaabdhismeedkeeda molecular waxay la mid tahay antibiyootiga aminoglycoside.Polarity ee muunad ayaa ahaa mid aad u sarreeya, taas oo ka dhigaysa mid aad ugu milmay biyaha.Jaantuska jaantuska qaabdhismeedka molecular ee muunadda ayaa lagu muujiyay sawirka 1. Nadiifnimada muunada cayriin waxay ahayd ilaa 88% sida ay u falanqaysay HPLC.Si loo nadiifiyo xeryahooda polarity sare, muunada si dhib yar ayaa loogu hayn doonaa tiirarka caadiga ah ee C18 marka loo eego khibradaheenii hore.Sidaa darteed, tiir C18AQ ah ayaa loo shaqaaleysiiyay muunadda nadiifinta.
Jaantuska 1. Jaantuska jaantuska qaabdhismeedka molecular ee muunadda.
Si loo diyaariyo xal muunada, muunad cayriin mg 50 ayaa lagu kala diri 5 mL biyo saafi ah ka dibna ultrasonicated si ay u noqoto xal gebi ahaanba cad.Xalka muunada ayaa markaa lagu duray tiirka flashka iyadoo la durayo.Habaynta tijaabada ah ee nadiifinta tooshku waxay ku taxan tahay shaxda 1.
| Qalabka | SepaBean™ mashiinka 2 | |
| Kartridges | 12 g SepaFlash C18AQ RP flash cartridge (silica wareegsan, 20 - 45μm, 100 Å, Lambarka dalbashada: SW-5222-012-SP(AQ)) | |
| Mawjada dhererka | 204 nm, 220 nm | |
| Marxaladda mobilada | Walxaha A: Biyo Xalka B: Acetonitrile | |
| Heerka socodka | 15 ml/min | |
| Muunad rarista | 50 mg | |
| Gradient | Waqtiga (min) | Miraha B (%) |
| 0 | 0 | |
| 19.0 | 8 | |
| 47.0 | 80 | |
| 52.0 | 80 | |
Natiijooyinka iyo dood
chromatogram-ka tooska ah ee muunadda ku taal kartoonka C18AQ ayaa lagu muujiyey sawirka 2. Sida ku cad sawirka 2, muunadda aadka u saraysa ayaa si wax ku ool ah loogu hayaa sanduuqa C18AQ.Ka dib lyopholization ee jajabyada la ururiyey, badeecada la beegsanayo waxay lahayd nadiif ah 96.2% (sida ku cad sawirka 3) ee falanqaynta HPLC.Natiijooyinka waxay muujiyeen in badeecada la sifeeyay laga faa'iidaysan karo tallaabada xigta ee cilmi-baarista iyo horumarinta.
Jaantuska 2. Kormatogaraamka toosh ee muunada ee ku dul yaal kartuunada C18AQ.
Jaantuska 3. chromatogramka HPLC ee alaabta la beegsanayo.
Gebogebadii, SepaFlash C18AQ RP flash cartridge oo ay weheliso nidaamka chromatography-ga ee mashiinka SepaBean ™ wuxuu ku siin karaa xal degdeg ah oo wax ku ool ah sifaynta muunado aadka u sarreeya.
Ku saabsan SepaFlash C18AQ RP flash cartridges
Waxaa jira taxane ah kartoonada flash-ka ee SepaFlash C18AQ RP oo leh tilmaamo kala duwan oo ka yimid Tignoolajiyada Santai (sida ku cad Shaxda 2).
| Lambarka Shayga | Cabirka tiirka | Heerka socodka (ml/min) | Ugu badnaan.cadaadiska (psi/bar) |
| SW-5222-004-SP(AQ) | 5.4 g | 5-15 | 400/27.5 |
| SW-5222-012-SP(AQ) | 20 g | 10-25 | 400/27.5 |
| SW-5222-025-SP(AQ) | 33 g | 10-25 | 400/27.5 |
| SW-5222-040-SP(AQ) | 48 g | 15-30 | 400/27.5 |
| SW-5222-080-SP(AQ) | 105 g | 25-50 | 350/24.0 |
| SW-5222-120-SP(AQ) | 155 g | 30-60 | 300/20.7 |
| SW-5222-220-SP(AQ) | 300 g | 40-80 | 300/20.7 |
| SW-5222-330-SP(AQ) | 420 g | 40-80 | 250/17.2 |
Shaxda 2. SepaFlash C18AQ RP flash cartridges.Qalabka wax lagu baakadeeyo: Waxtarka spherical C18 (AQ) ee silica isku xiran, 20 - 45 μm, 100 Å.
Macluumaad dheeri ah oo ku saabsan tifaftirayaasha faahfaahsan ee mashiinka SepaBean™, ama macluumaadka dalbashada ee sepaFlash, fadlan booqo shabakadayada.
Waqtiga boostada: Oct-26-2018