Rui Huang, Bo Xu
Codsiga Xarunta R&D
Hordhac
Ion beddelka chromatography (IEC) waa hab chromatographic ah oo inta badan loo isticmaalo in lagu kala saaro oo lagu nadiifiyo xeryahooda kuwaas oo lagu soo bandhigay qaabka ionic ee xalka.Marka loo eego xaaladaha kala duwan ee khidmadaha ee ions la beddeli karo, IEC waxa loo qaybin karaa laba nooc, chromatography beddelashada cation iyo chromatography beddelka anion.Kormatography beddelashada cation, kooxaha acidic waxay ku xidhan yihiin oogada warbaahinta kala soocida.Tusaale ahaan, sulfonic acid (-SO3H) waa koox caadi ahaan loo isticmaalo isweydaarsiga isdhaafsiga xooggan (SCX), kaas oo kala saara H+ iyo kooxda si xun u dacwooday -SO3- waxay sidaas ku dhejin kartaa cations kale ee xalka.chromatography beddelashada anion, kooxaha alkaline waxay ku xidhan yihiin dusha sare ee warbaahinta kala go'a.Tusaale ahaan, amine quaternary (-NR3OH, halka R ay tahay kooxda hydrocarbon) waxaa badanaa loo isticmaalaa is-weydaarsiga anion-ka xooggan (SAX), kaas oo kala sooca OH- iyo kooxda si fiican loo dallaco -N + R3 waxay ku dhejin kartaa anions kale ee xalka, taasoo keentay anion saamaynta beddelka.
Waxyaabaha dabiiciga ah, flavonoids ayaa soo jiitay dareenka cilmi-baarayaasha sababtoo ah doorka ay ku leeyihiin ka hortagga iyo daaweynta cudurrada wadnaha iyo xididada.Maadaama molecules flavonoid ay aashito ay sabab u tahay joogitaanka kooxaha phenolic hydroxyl, chromatography beddelashada ion waa ikhtiyaarka beddelka ah marka lagu daro wejiga caadiga ah ee caadiga ah ama chromatography wejiga roga ee kala-soocidda iyo nadiifinta xeryahooda asiidhka ah.In chromatography flash, warbaahinta sida caadiga ah loo isticmaalo kala soocida for ion beddelka waa silica gel matrix halkaas oo kooxaha sarrifka ion ay ku xidhan yihiin korkeeda.Hababka beddelka ion ee ugu badan ee loo isticmaalo chromatography flash waa SCX (badanaa kooxda sulfonic acid) iyo SAX (sida caadiga ah kooxda aminiga quaternary).Qoraalkii hore ee arjiga la daabacay ee cinwaankiisu ahaa "Codsiga SepaFlash Strong Cation Columns ee Nadaafadda Isku-dhafka Alkaline" ee Santai Technologies, tiirarka SCX ayaa loo shaqaaleysiiyay nadiifinta xeryahooda alkaline.Boostada, isku dhafka heerarka dhexdhexaadka ah iyo aashitada ayaa loo adeegsaday muunada si loo sahamiyo codsiga tiirarka SAX ee nadiifinta xeryahooda acidic.
Qaybta Tijaabada
Jaantuska 1. Jaantuska jaantuska ee marxaladda taagan ee ku xidhan dusha sare ee warbaahinta kala-soocidda SAX.
Boostada dhexdeeda, tiir SAX oo hore loogu sii diyaariyay silica quaternary amine bonded ayaa la isticmaalay (sida ku cad sawirka 1).Isku darka Chromone iyo 2,4-dihydroxybenzoic acid ayaa loo isticmaalay muunada si loo nadiifiyo (sida ku cad sawirka 2).Isku darka waxaa lagu milmay methanol waxaana lagu soo shubay kartuun flashka duriste.Habaynta tijaabada ah ee nadiifinta tooshku waxay ku taxan tahay shaxda 1.
Jaantuska 2. Qaabdhismeedka kiimikada ee labada qaybood ee isku dhafka muunada.
| Qalabka | SepaBean™ mashiinka T | |||||
| Kartridges | 4 g SepaFlash Standard Series flash kartooj (silica aan joogto ahayn, 40 - 63 μm, 60 Å, Lambarka dalbashada: S-5101-0004) | 4 g SepaFlash Bonded Series SAX flash cartridge (silica aan joogto ahayn, 40 - 63 μm, 60 Å, Lambarka dalbashada: SW-5001-004-IR) | ||||
| Mawjada dhererka | 254 nm (ogaanshaha), 280 nm (kormeerka) | |||||
| Marxaladda mobilada | Xalka A: N-hexane | |||||
| Xalka B: Ethyl acetate | ||||||
| Heerka socodka | 30 ml/daqiiqo | 20 ml/min | ||||
| Muunad rarista | 20 mg (isku dar ah Qaybaha A iyo Qaybta B) | |||||
| Gradient | Waqtiga (CV) | Miraha B (%) | Waqtiga (CV) | Miraha B (%) | ||
| 0 | 0 | 0 | 0 | |||
| 1.7 | 12 | 14 | 100 | |||
| 3.7 | 12 | / | / | |||
| 16 | 100 | / | / | |||
| 18 | 100 | / | / | |||
Natiijooyinka iyo dood
Marka hore, isku darka muunada waxaa lagu kala saaray kartridge wajiga caadiga ah oo horay loogu sii diyaariyay silica caadiga ah.Sida ku cad Jaantuska 3, labada qaybood ee muunada ayaa laga soo saaray kartridge midba midka kale.Marka xigta, kartridge SAX ah ayaa loo isticmaalay sifaynta muunadda.Sida ku cad Jaantuska 4, Qaybta Aashitada B ayaa si buuxda loogu hayaa sanduuqa SAX.Qaybta dhexdhexaadka ah ee A ayaa si tartiib tartiib ah looga saaray kartoonka iyada oo la iftiimiyay wajiga wareega.
Jaantuska 3. Karamatogaraamka toosh ee shaybaarka oo ku yaal kartoonka wejiga caadiga ah ee caadiga ah.
Jaantuska 4. Kormatogaraamka toosh ee shaybaarka ee ku jira kartoonka SAX.
Isbarbardhigga Jaantuska 3 iyo Jaantuska 4, Qaybta A waxa ay leedahay qaab aan is waafaqsanayn oo ku saabsan labada kartoon ee kala duwan.Si loo xaqiijiyo in heerka ugu sarreeya uu u dhigmo qaybta, waxaan ka faa'iidaysan karnaa sawirka mowjadda dhererka buuxa kaas oo lagu dhex dhisay software-ka kantaroolka ee mashiinka SepaBean™.Fur xogta tijaabada ee labada kala soocida, u jiid xariiqda tilmaame ee dhidibka wakhtiga (CV) ee chromatogramka ilaa meesha ugu sarraysa iyo barta labaad ee ugu sarraysa ee ugu sarraysa ee u dhiganta Qaybta A, iyo mawjada dhererka buuxa ee labadan Dhibcaha si toos ah ayaa loo muujin doonaa ka hooseeya chromatogramka (sida ku cad sawirka 5 iyo sawirka 6).Isbarbardhigga xogta mawjada dhererka buuxa ee labadan kala sooc, Qaybta A waxay leedahay nuugista spectrum joogto ah laba tijaabo.Sababta Qaybta A waxay u leedahay qaab aan iswaafaqayn oo ugu sarreysa laba kartoon oo kala duwan, waxaa la qiyaasayaa in ay jirto wasakh gaar ah oo ku jirta Qaybta A oo leh haynta kala duwan ee kartoojka wajiga caadiga ah iyo kartoonka SAX.Sidaa darteed, isku xigxiga sare u kaca waa uu ka duwan yahay Qaybta A iyo wasakhnimada ku jirta labadan kartoon ee flash-ka, taasoo keentay qaabka ugu sarreeya ee koromogaramyada.
Jaantuska 5. Mawjada dhererka buuxa ee Qaybta A iyo wasakhda ay kala soocaan kartoonka heerka caadiga ah.
Jaantuska 6. Mawjada dhererka buuxa ee Qaybta A iyo wasakhda ay kala soocaan kartoonada SAX.
Haddii sheyga la rabo in la ururiyo uu yahay qaybta dhexdhexaadka ah ee A, hawsha nadiifinta si fudud ayaa loo dhamaystiri karaa iyadoo si toos ah loo isticmaalo kartoonka SAX si loo iftiimiyo ka dib marka muunada la raro.Dhanka kale, haddii badeecada bartilmaameedka ah ee la ururinayo ay tahay qaybta acidic B, habka soo-dejinta waxaa lagu qaadan karaa oo keliya hagaajin yar oo tillaabooyinka tijaabada ah: marka muunada lagu shubo kartoonka SAX iyo qaybta dhexdhexaadka ah ee A. waxaa si buuxda looga saaray xalalka caadiga ah ee dabiiciga ah, u beddel wejiga guurguura xal methanol ka kooban 5% acetic acid.Iions acetate ee wajiga wareegta waxay la tartami doonaan Qaybta B si loogu xidho kooxaha amine ion ee quaternary ee wejiga taagan ee SAX cartridge, taas oo ka soo baxaysa Qaybta B ee kartoojka si loo helo badeecada la beegsanayo.chromatogram-ka muunadda lagu kala soocay habka beddelka ion waxa lagu muujiyay sawirka 7.
Jaantuska 7. Koromatogaramka iftiishka ah ee Qaybta B ayaa lagu soo saaray habka beddelka ion ee kartoonka SAX.
Gebogebadii, muunadda aashitada ama dhexdhexaadka ah ayaa si degdeg ah loogu nadiifin karaa kartoonka SAX oo lagu daray kartoonka wajiga caadiga ah iyadoo la adeegsanayo xeelado kala duwan oo nadiifin ah.Intaa waxaa dheer, iyada oo la kaashanayo sawirka mowjadda dhererka buuxa ee lagu dhex dhisay software-ka kantaroolka ee mashiinka SepaBean ™, qaabka nuugista sifada ee jajabyada sare loo qaaday si fudud ayaa loo barbar dhigi karaa oo la xaqiijin karaa, ka caawinta cilmi-baarayaasha si dhakhso ah u go'aamiyaan halabuurka iyo nadiifnimada jajabyada la jeexjeexay hufnaanta shaqada.
| Lambarka Shayga | Cabirka tiirka | Heerka socodka (ml/min) | Ugu badnaan.cadaadiska (psi/bar) |
| SW-5001-004-IR | 5.9 g | 10-20 | 400/27.5 |
| SW-5001-012-IR | 23 g | 15-30 | 400/27.5 |
| SW-5001-025-IR | 38 g | 15-30 | 400/27.5 |
| SW-5001-040-IR | 55 g | 20-40 | 400/27.5 |
| SW-5001-080-IR | 122 g | 30-60 | 350/24.0 |
| SW-5001-120-IR | 180 g | 40-80 | 300/20.7 |
| SW-5001-220-IR | 340 g | 50-100 | 300/20.7 |
| SW-5001-330-IR | 475 g | 50-100 | 250/17.2
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Shaxda 2. SepaFlash Bonded Series SAX flash cartridges.Qalabka wax lagu xidho: Silica SAX-ku-xidhan ee aan joogto ahayn ee aadka u saafiga ah, 40 - 63 μm, 60 Å.
Wixii macluumaad dheeraad ah oo ku saabsan faahfaahinta faahfaahsan ee SepaBean™mashiinka, ama macluumaadka dalbashada ee sepaFlash taxane flash kartooj, fadlan booqo website-kayaga.
Waqtiga boostada: Nov-09-2018