Rui Huang, Bo Xu
Codsiga R & D Center
Gogoldhig
Iibsiga ION Chromatography (IEC) waa hab chromanograacis ah oo caadi ahaan loo isticmaalo in lagu kala sooco loona daahiriyo xeryahooda oo lagu soo bandhigay foomka ionic ee xalka. Sida laga soo xigtay qarashka qarashka kaladuwan ee ioraanyada la isweydaarsanayo, IEC waxaa loo qaybin karaa laba nooc, isdhaafsiga isdhaafsiga is-dhaafsiga iyo isdhaafsiga anasion. Iibiyaha isdhaxgalka isdhaxgalka, kooxaha aashitada ayaa loo xiraa dusha sare ee warbaahinta kala go'ida. Tusaale ahaan, sulfonic acid (-3h) waa koox caadi ah oo la istcimaalo isweydaarsiga adag (SCX), oo loo diro H + iyo kooxda si xun loo soo dalacayo - sidaas darteedna adsorb-ka kale xalka. Anion isweydaarsiga kombiyuutarka, kooxaha alkaline-ka ayaa loo xiraa dusha sare ee warbaahinta kala go'ida. Tusaale ahaan, digaagga kaxeynta iyo amine (-nr3, halka ver-ka lagu isticmaalo isdhaafsiga anion ah), kuwaas oo si togan ah ee lagu eedeeyo xalka kale ee xalka, taasoo dhalisay saameynta isdhaafsiga anion.
Alaabada dabiiciga ah, flavonoids waxay soo jiidatay dareenka cilmi-baarayaasha sababta oo ah doorkooda ka haynta ka hortagga iyo daaweynta cudurada wadnaha. Maaddaama molecules molecules flavonoid ay tahay aashitada ay sabab u tahay jiritaanka hydroxyl kooxo hydroxyl, ION Chromatography waa ikhtiyaar kale marka lagu daro wajiga caadiga ah ee caadiga ah ama dib-u-eegista korodhka kontoroolka iyo nadiifinta xeryahooda aashitada. In Flash-ka Flash-ka, warbaahinta kala go'ida ee caadiga ah ee isweydaarsiga Ion Gel Matrix halkaas oo ION GELO's Extress's ee dusha sare. Hababka ugu badan ee loo adeegsado ION ee isdhaafsiga 'Flash-ka' waa scx (badiyaa kooxda sulfonic acid) iyo sax (badiyaa waaxda kabaha ka soo gaarta dadka ka xula karta dadka ka baxsan. Ogeysiiska arjiga ee horay loo daabacay ee cinwaanka "Codsiga SEPEFLASH SITE CHITION CHITIONIMITNIMIMIMITINMITINAINAINAINAINAINAINAINAINAINAINAINAINAINAINAINAINAINAINSKA CARRUURTA XIRIIRKA KHUDBADAHA" Waxaa qoray Stantaine Technologists, tiirarka SCX waxay u shaqaysay nadiifinta xeryahooda alkaline. Qoraalkan, isku-darka heerarka dhexdhexaad ee dhexdhexaad ah iyo aashitada ayaa loo isticmaalay in loo qaado muunadda si loo sahariyo arjiga sadarka sax ee lagu nadiifinayo xeryahooda aashitada.
Qaybta tijaabada
Jaantus 1. Jaantusyada qorshaynta wejiga taagan ee wejiga taagan ee ku xidhnaa dusha sare ee warbaahinta SAX.
Qoraalkan, saxarka sax oo horay loo soo buuxiyay dadka ka soo xumaado ka soo gaaray xubnaha ka mid ah xubnaha ka mid ah silica silica ee loo xiray (sida lagu muujiyey Jaantuska 1). Isku dar ah chromone iyo 2,4-dihydroxybenbenbenbenzic acid waxaa loo isticmaalay in ah muunad in la nadiifiyo (sida lagu muujiyey Jaantuska 2). Isku dar ah ayaa lagu kala diray Methayl waxayna ku rakibtay sanduuqa tooshka ah ee dushiisa. Dejinta tijaabada ah ee lagu nadiifiyo Flash-ka ayaa ku qoran shaxda 1.
Jaantus 2. Qaab dhismeedka kiimikada ee labada qaybood ee isku-darka muunadda.
| Qalab | Mashiinka Sepabisean ™ t | |||||
| Cartroid | 4 G sepaflash Seacation Standard Sent Flash Shurtridge (silig aan caadi ahayn, 40 - 63 μm, 60 Å, lambarka dalbashada: S-5101-000-0004-0004-0004-0004-000-0004-0004-000-0004-000-0004) | 4 g sepeflash-ka SAX Flat SAX Flash Sartridge (silica silica oo aan caadi ahayn, 40 - 63 μm, 60 Å 5 Å 5001-004- IR) | ||||
| Shaab maamuus | 254 NM (ogaanshaha), 280 NM (kormeer) | |||||
| Wajiga mobilada | Dareenka a: n-hexene | |||||
| Solvent B: Etyl acetate | ||||||
| Sicirka qulqulka | 30 ml / min | 20 ml / min | ||||
| Muunad qaadida | 20 mg (isku dar ah qayb ka kooban a iyo qayb ah b) | |||||
| Heer deg-degoobid | Waqtiga (CV) | XULASHADA B (%) | Waqtiga (CV) | XULASHADA B (%) | ||
| 0 | 0 | 0 | 0 | |||
| 1.7 | 12 | 14 | 100 | |||
| 3.7 | 12 | / | / | |||
| 16 | 100 | / | / | |||
| 18 | 100 | / | / | |||
Natiijooyinka iyo wada hadalka
Marka hore, isku-darka muunadda ayaa lagu kala soocay sanduuqa faashad ee caadiga ah ee ka hor lagu buuxay silica joogto ah. Sida show Jaantuska 3, labada qaybood ee muunadda lagu soo qaaday ayaa laga soo qaatay baararka mid kale ka dib. Marka xigta, xarkaha fallaagada sax ayaa loo adeegsaday nadiifinta muunadda. Sida muuqata Jaantuska 4, aashitada loo yaqaan 'aashitada' b gebi ahaanba lagu hayey baakada sax. Qeybta dhex-dhexaadka ah ee si tartiib tartiib ah ayaa looga soo qaatay kartoonka iyadoo laga dhawaaqay wejiga mobilada.
Jaantus 3. Tooshka Flash-ka ee muunadda ee muunadda ee ku yaal Shuruudaha Marxaladda caadiga ee caadiga ah.
Jaantus 4. Tooshka Flash-ka ah ee muunadda ah ee muunadda ku yaal kartoonada sax.
Isbarbardhiga Jaantuska 3 iyo Jaantus 4, Qeybta 4aad, waa qeyb ka mid ah qaabkii ugu sarreysa ee ugu sarreeya ee labada xargaha Flash Flash. Si loo xaqiijiyo in ugu sarreysa ee xasuuqiisii ay u egtahay qaybta, waxaan u adeegsan karnaa astaamaha iskaanka wanooyinka oo buuxa oo lagu dhisay software-ka kombiyuutarka ee mashiinka sepabean ™. Fur xogta tijaabada ah ee labada kala goynta, u jiid xariijinta tilmaamaha ee waqtiga dhididka (CV) ee ah meesha ugu sarreysa ee ugu sareysa ee ugu muuqata mid ka hooseeya chromaterkan (sida lagu muujiyey Jaantuska 5). Isbarbardhigaya xaraashka wavotrum-ka buuxa ee labadaas kala go'ay, qaybtaas waxay ka kooban tahay mid ka kooban laba tijaabo oo laba tijaabo ah. Sababta oo ah qayb ka kooban qaab heer sare ah oo ku saabsan laba kaarboor oo kala duwan oo kala duwan, waxaa loo cayimay inay jiraan wax qaas ah oo ka kooban qeybta ay ku hayaan xarkaha wajiga ee caadiga ah iyo baakada sax. Sidaa darteed, taxanaha baxsiga ayaa ka duwan qayb ka kooban a iyo nijaasta ah ee labadan xirmooyinka tooska ah, taasoo dhalisay qaab peak-ka aan iswaafaqsaneyn ee koromomanka.
Jaantus 5
Jaantus 6
Haddii badeecada bartilmaameedka la soo aruuriyo ay tahay qaybta dhexdhexaad ah ee a, shaqadu si fudud ayaa loo buuxin karaa si toos ah iyadoo si toos ah loo adeegsanayo SAX Chrotge si loo muujiyo ka dib xamuulka muunadda ka dib qaadista saamiga. Dhanka kale, haddii badeecada bartilmaameedka la soo aruuriyo ay tahay qaybta aashitada b, habka loo sii-dari karo oo keliya isbadal yar oo ka mid ah xalka unugyada caadiga ah, oo ah aaladda methanol xalka methanol ee ka kooban 5% acetic acid. IOM MERTATE MESSENT waxay la tartami doontaa qaybta ay ku xidhantahay kooxaha ka mid ah kooxaha ka dhashay ka soo horjeedka ee wajiga SAX CARRUURTA, taas oo ku celcelinaysa qaybta 'B' '' '' '' '' kartridge '. Chromaterag-ka ee muunadda laga soocay ee habka isdhaafsiga ee Ion ayaa lagu muujiyey Jaantuska 7.
Jaantus 7. Tooshka Flash-ka ee 'Chromamaragga' Flash 'ee qaybta ayaa lagu soo afjaray qaabka Ion isweydaarsiga ee ku yaal kartoonada sax.
Gunaanad, muunad dhexdhexaad ah ama muunad dhexdhexaad ah ayaa si dhakhso ah loogu daahi karaa Karttridge oo ay weheliso mastridge-ka caadiga ah ee caadiga ah ee loo adeegsado istiraatiijiyad nadiif ah. Intaa waxaa sii dheer, iyadoo la kaashanayo muuqaalka iskaanka wanaagga oo buuxa ee mashiinka kombiyuutarka ee SEPABEAN ™, oo ah ka caawinta cilmi-baarayaasha si dhakhso ah ayaa loo go'aamiyaa isku-darka iyo daahirka jajabyada la ansaxiyay sidaas darteedna hagaajinta hufnaanta shaqada.
| Lambarka Shayga | Cabbirka sadarka | Sicirka qulqulka (ml / min) | Max.pressure (Psi / bar) |
| Sw-5001-004-ir | 5.9 g | 10-20 | 400 / 27.5 |
| Sw-5001-012-ir | 23 g | 15-30 | 400 / 27.5 |
| Sw-5001-025-ir | 38 g | 15-30 | 400 / 27.5 |
| Sw-5001-040-ir | 55 g | 20-40 | 400 / 27.5 |
| Sw-5001-080-ir | 122 g | 30-60 | 350 / 24.0 |
| Sw-5001-120-ir | 180 g | 40-80 | 300 / 20.7 |
| Sw-5001-220-ir | 340 g | 50-100 | 300 / 20.7 |
| Sw-5001-330-ir | 475 g | 50-100 | 250 / 17.2
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Jadwalka 2aad Qalabka xirxirayaasha
Macluumaad dheeri ah oo ku saabsan faahfaahinta faahfaahsan ee Sepaben ™Mashiinka, ama macluumaadka dalabka ee taxanaha sepaflash-ka tooska ah, fadlan booqo bartayada internetka.
Waqtiga Post: Noven-09-2018