Hongcheng Wang, Bo Xu
Codsiga R & D Center
Gogoldhig
Sida laga soo xigtay faaruqadaha qaraabada ah ee wejiga taagan iyo wajiga mobilada, kontrografiyada dareeraha ah ayaa loo qaybin karaa chromase-ka caadiga ah (NPC) oo dib loo rogay wejiga hore ee koromada (RPC). RPC, polication of mobilada mobilada ayaa ka xoog badan kan wejiga taagan. RPC waxay noqotay mid sida ugu badan loo isticmaalo mid ka mid ah dareeraha chromacography-ga qaababka ka go'itaanka waxtarka sare, xalka wanaagsan iyo farsamada haynta ee cad. Sidaa darteed RPC waxay ku habboon tahay kala-goynta iyo nadiifinta xeryahooda kala duwan ama kuwa aan-ka-qaadin, oo ay ku jiraan aashito acids, amino acids, oo ay ku jiraan aashitada silica gel. Phenyl, Cyano, Amino, iwm Waxaa lagu qiyaasaa in ka badan 80% RPC ay hadda adeegsanayaan marxaladda C18. Sidaa darteed C18 Tiirka Chromacomka ee Chromacomka ayaa noqday in ay tahay in lagu haysto sadar caalami ah oo loogu talagalay sheybaar kasta.
In kasta oo sadarka C18 loo isticmaali karo codsiyo aad u tiro badan, si kastaba ha noqotee, saygaas qaar ka mid ah taab-yar ama biyo-biyo-gacmeedyo aad u badan, sadarrada C18 ee joogtada ah ayaa yeelan kara dhibaatooyin markii loo isticmaalay in lagu nadiifiyo saamballadaas. RPC gudaheeda, xayeysiinta ay si joogto ah loo isticmaalo ayaa lagu amri karaa iyada oo loo eegayo falalkiisa: Biyaha <methanol <acetonitrile <etranol <terrahydrofuran <ispropanol. Si loo hubiyo haynta wanaagsan ee safka sanballadan (pour xoog leh ama biyo-qabatin aad u sarreeya), cabir sare oo ah nidaamka aqueous waa lagama maarmaan in loo isticmaalo marxaladda mobilada. Si kastaba ha noqotee, markaad isticmaaleyso nidaamka biyaha saafiga ah (oo ay kujirto biyo saafi ah ama milix saafi ah) maadaama wajiga loo yaqaan 'c1' ee safka dheer ee safka C18 uu ka fogaadaa, taasoo dhalisay hoos udhaca, taasoo dhalisay in si dhakhso leh loo yareeyo xaga haynta ama haynta. Ifafaalahan waxaa loo yaqaan "wejiga wajiga" hydrophophobic-ka "(sida ka muuqata bidix ee Jaantuska 1). In kasta oo xaaladdan la beddeli karo markii tiirka lagu dhaqo solvents dabiici ah sida methanol ama acetonitrile, weli waxay u horseedi kartaa dhaawac safka. Sidaa darteed, waa lagama maarmaan in laga hortago xaaladdan inay dhacdo.
Jaantus 1. Jaantusyada Nidaamka ee wejiyada la xidhiidha dusha sare ee dusha silica gel ee safka caadiga ah (bidix) iyo sadarka C18AQ (midig).
Si wax looga qabto dhibaatooyinka kor lagu soo sheegay, soo-saareyaasha agabyada xirxirayaasha korontada ee soosaarayaasha ayaa sameeyay horumar farsamo. Mid ka mid ah horumarinnadan ayaa ka dhigeysa wax ka badal ah dusha sare ee silsiladda Silica matrix, sida soo bandhigida kooxaha hydrophilic (sida ka muuqata qaybta saxda ah ee Jaantuska 1), si looga dhigo dusha sare ee silica jeld hydrophilic. Markaa silsiladaha C18 ee dusha sare ee silsiladda silica ah ayaa si buuxda loogu dheerayn karaa xaalado aqueous ah oo aad u sareeya oo wejiga hydrophobic burburka waa laga fogaan karaa. Tiirarkan C18 ee wax laga beddelay waxaa loo yaqaannaa sadarka aqueous C18, oo ah tiirarka C18AQ, kuwaas oo loogu talagalay in si taxaddar leh loo baxo oo ay u dulqaadan karaan 100% nidaamka aqueous. Tiirarka C18AQ Tilmaamaha si ballaaran ayaa loogu dabaqay kala-goynta iyo nadiifinta xeryahooda xoogga badan, oo ay ku jiraan aashitada dabiiciga ah, acids-ka, nactos-ka iyo fiitamiinnada biyaha lagu milmay.
Desaling waa mid ka mid ah codsiyada caadiga ah ee sadarka C18AQ ee lagu nadiifiyo muunadaha, kaas oo ka saara milixda ama qaybaha wax lagu karsado ee tusaalaha tusaalaha ah ee sahlaynta muunadda ee daraasadaha xiga. Qoraalkan, FCF-ga quruxda badan ee FCF oo leh madadaalo xoog leh ayaa loo adeegsaday tusaalaha oo loo nadiifiyey sadarka C18AQ. Muunadda tusaalaha ah waxaa lagu bedelay dareeraha dabiiciga ah ee xalka xalka ah, sidaa darteed fududeynta qulqulka wareejinta soo socota iyo sidoo kale kaydinta xalka iyo waqtiga hawlgalka. Intaa waxaa sii dheer, daahirsanaanta muunadda ayaa la wanaajiyey iyadoo laga saarayo qaar ka mid ah ciladaha muunadda muunadda.
Qaybta tijaabada
Jaantus 2. Qaab dhismeedka kiimikada ee muunadda.
FCF-ga Blue ee dhalaalaya ee FCF waxaa loo istcimaalay muunadda jagadan. Daahirnimada muunadda ceyriinku waxay ahayd 86% qaab dhismeedka kiimikada ee muunadda lagu muujiyey Jaantuska 2-aad. Xalka tusaalaha ayaa markaa lagu muday safka tooshka ah ee dushiisa. Dejinta tijaabada ah ee lagu nadiifiyo Flash-ka ayaa ku qoran shaxda 1.
| Qalab | Mashiinka Sepabisean2 | |||
| Cartroid | 12 g sepaflash C18 rp Flash Chrotridge (spergical suul-galinta (Silica silica, 20 - 45 m, 100 Å 522222-012-sp) | 12 g sepeflash c18aq rp flafffridge (spersical protridge))) | ||
| Shaab maamuus | 254 nm | |||
| Wajiga mobilada | KOOXDA A: Biyo Solvent B: methanol | |||
| Sicirka qulqulka | 30 ml / min | |||
| Muunad qaadida | 300 mg (oo dhalaalaya fcf buluug ah oo daahir ah 86%) | |||
| Heer deg-degoobid | Waqtiga (CV) | XULASHADA B (%) | Waqtiga (CV) | XULASHADA B (%) |
| 0 | 10 | 0 | 0 | |
| 10 | 10 | 10 | 0 | |
| 10.1 | 100 | 10.1 | 100 | |
| 17.5 | 100 | 17.5 | 100 | |
| 17.6 | 10 | 17.6 | 0 | |
| 22.6 | 10 | 22.6 | 0 | |
Natiijooyinka iyo wada hadalka
SEPEFLASH C18AQ RP FLA FLUT CHTRIDGRADA Waxaa loo isticmaalay muunadda go'doon iyo nadiifin. Tallaabada ayaa loo adeegsaday in biyo nadiif ah loo isticmaalay in marxaladda mobilada ee bilowga laga jawaabayo loona maamulo 10 mugga tiirka (CV). Sida ka muuqata Jaantuska 3, markii la isticmaalayo biyo saafi ah sida wejiga gacanta, muunadda ayaa gabi ahaanba lagu hayey qadka Tooshka. Marka xigta, methanol ee wajiga mobilada ayaa si toos ah loo kordhiyay 100% oo heesta ayaa la ilaaliyay 7.5 cv. Shaybaarka waxaa laga soo qaatay 11.5 ilaa 13.5 CV. Jajabka aruurinta, xalka muunadda ayaa laga beddelay xalka nah2po4 ee Methanol. Isbarbar-dhigga xalka aqueous, methanol aad ayuu u sahlan yahay in laga saaro uumi-baxa wareegga talaabada xigta, kaas oo fududeeya cilmibaadhista soo socota.
Jaantus 3. Tooshka Tooshka Flash ee muunadda ee saamiga C18AQ Chrotridge.
In la isbarbar dhigo dhaqanka haynta ee C18Aq ee C18Aq Chrotridge iyo kartoonada C18 ee joogtada ah muunadaha loo yaqaan 'polaric adag', isbarbardhiga isbarbar dhig ee isbarbar dhiga ayaa la sameeyay. CEPAFLASH C18 RP Flash Chantridge ayaa la isticmaalay oo toosh koron oo muunad ah ayaa lagu muujiyey Jaantuska 4. Daryeello C18 oo caadi ah, saamiga ugu sareeya ee loo dulqaadanayo waa 90%. Sidaa darteed bilowga heerarka ayaa lagu dejiyey 10% methanol 90% biyaha. Sida ka muuqata Jaantuska 4, sababtuna tahay burburka wejiga ee dareeraha ah ee sababaha C18 ee sababay saamiga aqueous sare, muunadda waxaa si qunyar ah loogu hayey oo uu si toos ah u soo celiyay wejiga gacanta. Natiijo ahaan, qalliinka muunadda muunadda go'doominta ama nadiifinta lama dhammaystiri karo.
Jaantus 4. Tooshka Tooska ah ee muunadda ee muunadda ku jirta kartoonada C18 ee caadiga ah.
Isbarbar-dhijinta jilicsanaanta toosan, isticmaalka tillaabada tilaabada ayaa leh faa'iidooyinka soo socda:
1. Qabashada isticmaalka iyo waqtiga loo maro in lagu nadiifiyo muujinta ayaa la dhimay.
2. Waaxyada sheyga bartilmaameedka ah ee ugu sarreeya ee ugu sarreeya, kaas oo yareynaya mugga jajabyada aruurinta sidaas darteedna waxay fududeysaa uumi-baxa soo socda ee soo socda iyo sidoo kale waqtiga badbaadinta.
3. Wax soo saarka arjiga la soo aruuriyay wuxuu ku yaal Methanol kaas oo fududahay in la isticmaalo, sidaa darteed waqtiga qalajinta ayaa la dhimay.
Gabagabadii, nadiifinta muunadda oo si xoog leh u nadiifinta ama si weyn u neefso, sepaflash c18aq c18aq c18aq c18aq ™'s 'ee mashiinka' sepyraphean-ka 'sepabean-ka' sepabunean-ka 'sepabunean-ka' sepabunean-ka 'sepepabunean-ka' sepepabunean '.
Ku saabsan taxanaha sepaflash ee Sepeflash Setated C18 RP Flash Flash CaroTIDSS
Waxaa jira taxane ah oo ah sepeplash c18aq c18aq rp flafder kartoonnada iyada oo sharraxaad ah oo kala duwan oo ka socda tikniyoolajiyadda Statai (sida lagu muujiyey Shaxda 2).
| Lambarka Shayga | Cabbirka sadarka | Sicirka qulqulka (ml / min) | Max.pressure (Psi / bar) |
| SW-5222-004-sp (AQ) | 5.4 g | 5-15 | 400 / 27.5 |
| SW-5222-012-Sp (AQ) | 20 g | 10-25 | 400 / 27.5 |
| Sw-5222-025-sp (AQ) | 33 g | 10-25 | 400 / 27.5 |
| SW-5222-040-SP (AQ) | 48 g | 15-30 | 400 / 27.5 |
| SW-5222-080-SP (AQ) | 105 g | 25-50 | 350 / 24.0 |
| SW-5222-120-sp (AQ) | 155 g | 30-60 | 300 / 20.7 |
| SW-5222-220-sp (AQ) | 300 g | 40-80 | 300 / 20.7 |
| SW-5222-330-SP (AQ) | 420 g | 40-80 | 250 / 17.2 |
Jadwalka 2aad
Qalabka xirxirayaasha
logy (sida lagu muujiyey shaxda 2).
Waqtiga Post: Agoosto-27-2018