SepaFlash™ HP Fa'asologa
O koluma moli fa'asologa o HP o lo'o fa'apipi'iina ma fa'ataga mo le mamafa maualuga e o'o atu i le 400 psi. O mea fa'apipi'i avanoa e fa'afaigofie le feso'ota'iga ma so'o se faiga moli i luga o le maketi. O lenei fa'asologa e maua ai Luer-Lok i totonu ma Luer-Lok out fetu'una'i mo le fa'aputuina o koluma faigofie. A mua'i fa'apipi'i i le silica gel (e le masani ai, 25-40 μm, 60 Å; spherical, 20-45 μm, 70 Å), o lenei fa'asologa o lo'o fa'aalia ai se fa'ai'uga mata'ina i luga o kasini moli masani.
※ Filifili saoloto le silica le lelei poʻo le silica spherical e tusa ai ma lou manaʻoga
※ Faʻaleleia lelei fofo ma sili atu le gafatia o le utaina o faʻataʻitaʻiga
※ Spherical silica e maua ai le faʻaleleia atili o le faʻatinoga e aunoa ma le faʻateleina o le backpressure system
Mo le silica gel spherical, fa'atonuga lelei mai mea mata i oloa fa'auma fa'amautinoaina maualuga le tele-to-lot reproducibility ma fa'amautu fa'atonuga.
※ Fa'amaoni, fa'atuatuaina, toe gaosia
※ Leai se fa'aleagaina, maualalo le mamafa
※I'uga maualuga
※Tu'uga tutusa e leai se si'usi'u
※ Maualuga fa'ata'ita'iga fa'amomoli gafatia
Silika fa'aletonu maualuga, 25−40 µm, 60 Å(laueleele 500 m2/g, pH 6.5–7.5, fa'apipi'iina 0.1–15%)
Numera o mea | Tele o le koluma | Fa'ata'ita'iga Tele(g) | Fa'asolo (mL/min) | Umi ole pusa (cm) | ID pusa (mm) | Max. Malosi (psi/pa) | Aofaiga ile Pusa | |
Laiti | Tele | |||||||
SW-5102-004 | 4g | 4mg-0.6g | 15–30 | 113.8 | 12.4 | 400/27.5 | 24 | 120 |
SW-5102-012 | 12g | 12mg-1.8g | 25–50 | 134.8 | 21.4 | 400/27.5 | 24 | 108 |
SW-5102-025 | 25g | 25mg-3.8g | 25–50 | 184 | 21.4 | 400/27.5 | 18 | 72 |
SW-5102-040 | 40g | 40mg-6.0g | 30–60 | 184.4 | 26.7 | 400/27.5 | 12 | 48 |
SW-5102-080 | 80g | 80mg-12g | 40–80 | 257.4 | 31.2 | 350/24.0 | 10 | 20 |
SW-5102-120 | 120g | 120mg-18g | 45–90 | 261.5 | 38.6 | 300/20.7 | 8 | 16 |
SW-5102-220 | 220g | 220mg-33g | 60–120 | 223.5 | 61.4 | 300/20.7 | 4 | 8 |
SW-5102-330 | 330g | 330mg-50g | 60–120 | 280.2 | 61.4 | 250/17.2 | 3 | 6 |
SW-5102-800 | 800g | 800mg-120g | 50-100 | 382.9 | 78.2 | 100/6.9 | 3 | - |
SW-5102-1600 | 1600g | 1.6g-240g | 50-100 | 432.4 | 103.8 | 100/6.9 | 2 | - |
SW-5102-3000 | 3kg | 3g-450g | 50-100 | 509.5 | 127.5 | 100/6.9 | 1 | - |
SW-5102-5000 | 5kg | 5g-750g | 50-100 | 770 | 127.5 | 100/6.9 | 1 | - |
SW-5102-010K | 10kg | 10g-1.5kg | 50-100 | 850 | 172.5 | 100/6.9 | 1 | - |
※ Faʻatasi ma faiga faʻapipiʻi chromatography uma i luga o le maketi.
O le fa'amamāina o mea fa'afefiloi e faigata ona tu'u'ese'ese e ala i le fa'amalama chromatography (ΔRf ≤ 0.2 i le va o fa'ailoga i luga o le TLC) e masani ona i'u ai i laasaga fa'aopoopo e pei o le fa'amamāina mulimuli ane e ala ile fua sauniuni HPLC. E mafai ona fa'aitiitia le aofa'i o galuega fa'aopoopo e mana'omia mo le fa'amamāina i le na'o le fa'aputuina o ni koluma SepaFlash™ ua uma ona fa'apipi'i fa'ai'u i luga o se faiga fa'amalama chromatography.
I le chromatography vai, e vavae ese ituaiga o vailaʻau i luga o le faʻavae o latou eseesega i le saoasaoa aʻo latou fealuaʻi i totonu o le koluma. Ole fa'ateleina ole umi ole koluma e mafai ona matua fa'atuputeleina ai fa'aiuga. E ala i le fa'aputuina o koluma pito i tua o le umi i le lautele (L i le D) e fa'atuputeleina ina ia leai ni suiga tetele i le aufaasālalau ma le solvent system e mana'omia. E masani lava o lenei faateleina L i le D ua lava lea e maua ai le vavaeeseina manuia o paluga faigata ona o le tapunia o le taimi o le taofiga e le maua i luga o se koluma se tasi. O fa'amaumauga o lo'o fa'aalia ai le feso'ota'iga laina i le va o le fa'ai'uga ma le umi atoa o koluma.
Silika lapotopoto mama, 40−75 µm, 70 Å(laueleele 500 m2/g, pH 6.0–8.0, fa'apipi'i gafatia 0.1–10%)
Numera o mea | Tele o le koluma | Fa'ata'ita'iga Tele(g) | Fa'asolo (mL/min) | Umi ole pusa (cm) | ID pusa (mm) | Max. Malosi (psi/pa) | Aofaiga ile Pusa | |
Laiti | Tele | |||||||
SW-2101-004-SP | 4g | 4mg–0.4g | 15–40 | 113.8 | 12.4 | 400/27.5 | 24 | 120 |
SW-2101-012-SP | 12g | 12mg–1.2g | 30–60 | 134.8 | 21.4 | 400/27.5 | 24 | 108 |
SW-2101-025-SP | 25g | 25mg–2.5g | 30–60 | 184 | 21.4 | 400/27.5 | 18 | 72 |
SW-2101-040-SP | 40g | 40mg–4.0g | 40–70 | 184.4 | 26.7 | 400/27.5 | 12 | 48 |
SW-2101-080-SP | 80g | 80mg–8.0g | 50–100 | 257.4 | 31.2 | 350/24.0 | 10 | 20 |
SW-2101-120-SP | 120g | 120mg–12g | 60–150 | 261.5 | 38.6 | 300/20.7 | 8 | 16 |
SW-2101-220-SP | 220g | 220mg–22g | 80–220 | 223.5 | 61.4 | 300/20.7 | 4 | 8 |
SW-2101-330-SP | 330g | 330mg–33g | 80–220 | 280.2 | 61.4 | 250/17.2 | 3 | 6 |
SW-2101-800-SP | 800g | 800mg-80g | 50-100 | 382.9 | 78.2 | 100/6.9 | 3 | - |
SW-2101-1600-SP | 1600g | 1.6g-160g | 50-100 | 432.4 | 103.8 | 100/6.9 | 2 | - |
SW-2101-3000-SP | 3kg | 3g-300g | 50-100 | 509.5 | 127.5 | 100/6.9 | 1 | - |
SW-2101-5000-SP | 5kg | 5g-500g | 50-100 | 770 | 127.5 | 100/6.9 | 1 | - |
SW-2101-010K-SP | 10kg | 10g-1kg | 50-100 | 850 | 172.5 | 100/6.9 | 1 | - |
※ Fa'atasi ma faiga fa'amalama chromatography uma i luga o le maketi.
Silika lapotopoto maualuga, 20−45 µm, 70 Å(laueleele 500 m2/g, pH 6.0–8.0, fa'amomoli gafatia 0.1–15%)
Numera o mea | Tele o le koluma | Fa'ata'ita'iga Tele(g) | Fa'asolo (mL/min) | Umi ole pusa (cm) | ID pusa (mm) | Max. Malosi (psi/pa) | Aofaiga ile Pusa | |
Laiti | Tele | |||||||
SW-2102-004-SP | 4g | 4mg–0.6g | 15–30 | 113.8 | 12.4 | 400/27.5 | 24 | 120 |
SW-2102-012-SP | 12g | 12mg–1.8g | 25-50 | 134.8 | 21.4 | 400/27.5 | 24 | 108 |
SW-2102-025-SP | 25g | 25mg–3.8g | 25-50 | 184 | 21.4 | 400/27.5 | 18 | 72 |
SW-2102-040-SP | 40g | 40mg–6.0g | 30–60 | 184.4 | 26.7 | 400/27.5 | 12 | 48 |
SW-2102-080-SP | 80g | 80mg–12g | 40–80 | 257.4 | 31.2 | 350/24.0 | 10 | 20 |
SW-2102-120-SP | 120g | 120mg–18g | 45–90 | 261.5 | 38.6 | 300/20.7 | 8 | 16 |
SW-2102-220-SP | 220g | 220mg–33g | 60–120 | 223.5 | 61.4 | 300/20.7 | 4 | 8 |
SW-2102-330-SP | 330g | 330mg–50g | 60–120 | 280.2 | 61.4 | 250/17.2 | 3 | 6 |
SW-2102-800-SP | 800g | 800mg-120g | 50-100 | 382.9 | 78.2 | 100/6.9 | 3 | - |
SW-2102-1600-SP | 1600g | 1.6g-240g | 50-100 | 432.4 | 103.8 | 100/6.9 | 2 | - |
SW-2102-3000-SP | 3kg | 3g-450g | 50-100 | 509.5 | 127.5 | 100/6.9 | 1 | - |
SW-2102-5000-SP | 5kg | 5g-750g | 50-100 | 770 | 127.5 | 100/6.9 | 1 | - |
SW-2102-010K-SP | 10kg | 10g-1.5kg | 50-100 | 850 | 172.5 | 100/6.9 | 1 | - |
※ Fa'atasi ma faiga fa'amalama chromatography uma i luga o le maketi.
Silica lapotopoto maualuga, 50 μm, 54 Å(vaega i luga ole laiga 700 m2/g, pH 6.0‒8.0, uta gafatia 0.1‒30%)
Numera o mea | Tele o le koluma | Fa'ata'ita'iga Tele(g) | Fa'asolo (mL/min) | Umi ole pusa (cm) | ID pusa (mm) | Max. Malosi (psi/pa) | Aofaiga ile Pusa | |
Laiti | Tele | |||||||
SW-2101-004-SP(H) | 4g | 4mg–1.2g | 15–30 | 113.8 | 12.4 | 400/27.5 | 24 | 120 |
SW-2101-012-SP(H) | 12g | 12mg–3.6g | 25-50 | 134.8 | 21.4 | 400/27.5 | 24 | 108 |
SW-2101-025-SP(H) | 25g | 25mg–7.5g | 25-50 | 184 | 21.4 | 400/27.5 | 18 | 72 |
SW-2101-040-SP(H) | 40g | 40mg–12g | 30-60 | 184.4 | 26.7 | 400/27.5 | 12 | 48 |
SW-2101-080-SP(H) | 80g | 80mg–24g | 40-80 | 257.4 | 31.2 | 350/24.0 | 10 | 20 |
SW-2101-120-SP(H) | 120g | 120mg–36g | 45-90 | 261.5 | 38.6 | 300/20.7 | 8 | 16 |
SW-2101-220-SP(H) | 220g | 220mg–66g | 60-120 | 223.5 | 61.4 | 300/20.7 | 4 | 8 |
SW-2101-330-SP(H) | 330g | 330mg–99g | 60-120 | 280.2 | 61.4 | 250/17.2 | 3 | 6 |
SW-2101-800-SP(H) | 800g | 800mg-240g | 50-100 | 382.9 | 78.2 | 100/6.9 | 3 | - |
SW-2101-1600-SP(H) | 1600g | 1.6g-480g | 50-100 | 432.4 | 103.8 | 100/6.9 | 2 | - |
SW-2101-3000-SP(H) | 3kg | 3g-900g | 50-100 | 509.5 | 127.5 | 100/6.9 | 1 | - |
SW-2101-5000-SP(H) | 5kg | 5g-1.5kg | 50-100 | 770 | 127.5 | 100/6.9 | 1 | - |
SW-2101-010K-SP(H) | 10kg | 10g-3kg | 50-100 | 850 | 172.5 | 100/6.9 | 1 | - |
※ Fa'atasi ma faiga fa'amalama chromatography uma i luga o le maketi.
Silika lapotopoto maualuga, 25 µm, 50 Å(laueleele 700 m2/g, pH 5.0–8.0, fa'amomoli gafatia 0.1–30%)
Numera o mea | Tele o le koluma | Fa'ata'ita'iga Tele(g) | Fa'asolo (mL/min) | Umi ole pusa (cm) | ID pusa (mm) | Max. Malosi (psi/pa) | Aofaiga ile Pusa | |
Laiti | Tele | |||||||
SW-2102-004-SP(H) | 4g | 4mg–1.2g | 15–30 | 113.8 | 12.4 | 400/27.5 | 24 | 120 |
SW-2102-012-SP(H) | 12g | 12mg–3.6g | 25-50 | 134.8 | 21.4 | 400/27.5 | 24 | 108 |
SW-2102-025-SP(H) | 25g | 25mg–7.5g | 25-50 | 184 | 21.4 | 400/27.5 | 18 | 72 |
SW-2102-040-SP(H) | 40g | 40mg–12g | 30-60 | 184.4 | 26.7 | 400/27.5 | 12 | 48 |
SW-2102-080-SP(H) | 80g | 80mg–24g | 40-80 | 257.4 | 31.2 | 350/24.0 | 10 | 20 |
SW-2102-120-SP(H) | 120g | 120mg–36g | 45-90 | 261.5 | 38.6 | 300/20.7 | 8 | 16 |
SW-2102-220-SP(H) | 220g | 220mg–66g | 60-120 | 223.5 | 61.4 | 300/20.7 | 4 | 8 |
SW-2102-330-SP(H) | 330g | 330mg–99g | 60-120 | 280.2 | 61.4 | 250/17.2 | 3 | 6 |
SW-2102-800-SP(H) | 800g | 800mg-240g | 50-100 | 382.9 | 78.2 | 100/6.9 | 3 | - |
SW-2102-1600-SP(H) | 1600g | 1.6g-480g | 50-100 | 432.4 | 103.8 | 100/6.9 | 2 | - |
SW-2102-3000-SP(H) | 3kg | 3g-900g | 50-100 | 509.5 | 127.5 | 100/6.9 | 1 | - |
SW-2102-5000-SP(H) | 5kg | 5g-1.5kg | 50-100 | 770 | 127.5 | 100/6.9 | 1 | - |
SW-2102-010K-SP(H) | 10kg | 10g-3kg | 50-100 | 850 | 172.5 | 100/6.9 | 1 | - |
※ Fa'atasi ma faiga fa'amalama chromatography uma i luga o le maketi.
Silika lapotopoto maualuga, 15 µm, 50 Å(laueleele 700 m2/g, pH 5.0–8.0, fa'amomoli gafatia 0.1–30%)
Numera o mea | Tele o le koluma | Fa'ata'ita'iga Tele(g) | Fa'asolo (mL/min) | Umi ole pusa (cm) | ID pusa (mm) | Max. Malosi (psi/pa) | Aofaiga ile Pusa | |
Laiti | Tele | |||||||
SW-2103-004-SP(H) | 4 g | 4 mg–1.2 g | 10–15 | 113.8 | 12.4 | 400/27.5 | 24 | 120 |
SW-2103-012-SP(H) | 12 g | 12 mg–3.6 g | 15–20 | 134.8 | 21.4 | 400/27.5 | 24 | 108 |
SW-2103-025-SP(H) | 25 g | 25 mg–7.5 g | 15–20 | 184 | 21.4 | 400/27.5 | 18 | 72 |
SW-2103-040-SP(H) | 40 g | 40 mg–12 g | 20–30 | 184.4 | 26.7 | 400/27.5 | 12 | 48 |
SW-2103-080-SP(H) | 80 g | 80 mg–24 g | 30–40 | 257.4 | 31.2 | 350/24.0 | 10 | 20 |
SW-2103-120-SP(H) | 120 g | 120 mg–36 g | 35–45 | 261.5 | 38.6 | 300/20.7 | 8 | 16 |
SW-2103-220-SP(H) | 220 g | 220 mg–66 g | 50–65 | 223.5 | 61.4 | 300/20.7 | 4 | 8 |
SW-2103-330-SP(H) | 330 g | 330 mg–99 g | 50–65 | 280.2 | 61.4 | 250/17.2 | 3 | 6 |
※ Faʻatasi ma faiga faʻapipiʻi chromatography uma i luga o le maketi.
Fa'aluaina le Gafatia o Pusa o SepaFlash™ Cartridges na mua'i fa'atumu i le silica gel (700 m)2/g mo le 50 Å pore lapopoa) e maua ai le faatinoga aupito maualuga o le faamamaina. O le 25 μm poʻo le 15 μm silica spherical maualuga maualuga e iai le 40% maualuga maualuga, faʻaluaina le gafatia o le utaina o le silica pito i lalo. Na iloiloina e Santai le faatinoga o koluma SepaFlash™ pe a faatusatusa i ituaiga lauiloa e lua. O fa'ai'uga e fa'aalia ai le fa'asili ole SepaFlash™ i tagata tauva.
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