
Malo huang, bo xu
Talosaga R & D ogatotonu
Faʻafeiloaʻiga
O le peptide o se tuʻufaʻatasiga e aofia ai amino acids, o le mea e tasi o loʻo i ai i le tulaga tulaga ese o le tino ma vailaʻau ona o ituaiga eseese ma faʻasologa o le amno acid. Faʻatasi ai ma le atinaʻeina o vaega mautu vaega o vailaʻau, o le vailaʻau o loʻo faʻateleina ai le alualu i luma. Peitaʻi, ona o le faʻatusatusaina o tuʻufaʻatasiga o le ono maua mai i le vaega tumau o loʻo faʻamamaina e ala i le faʻatuatuaina o auala e faʻatuatuaina ai vaega faʻatatauina. O le masani ai faʻaaogaina metotia e faʻaaogaina mo le pept e aofia ai le tele o le chromonography (iec) o mea leaga o le utaina o le mole Tekinolosi, lea o le vavaeʻese o le diflash RP C18 Cartidge sa faʻaaogaina mo le saoasaoa o le faʻamamaina o Thomontin (TP-5) ma le sini o oloa na mauaina.
Ata 1. 20 POTO BALY AMIINO ACIDS (toe gaosia mai le www.bacham.com).
E 20 Ituaiga o amino acids e masani ai i le tuʻufaʻatasia o peptides. O nei amino acids e mafai ona vaevaeina i vaega nei e tusa ma la latou porlarity ma acid-basect meatotino: leai se porlar (hydror) I se faʻasologa o le peptide, pe a fai o le amino acids o loʻo avea ma se faʻasologa e tele lava o polar (pei ona faʻailogaina i luga o le vai 1). I le taimi o le faʻamaninoina o le faʻamaninoina mo nei vaega malosi o le poletini pptutd e ala i le faʻauiga o le chromomography, o le mea sa lolomiina muamua: Faʻatusatusa i le masani C18 koluma, o le faʻaleleia o C18Aq koluma e sili ona talafeagai mo le faʻamamaina o le malosi poʻo le hydrophilic faʻataʻitaʻiga faʻataʻitaʻiga. I lenei pou, o se malosi polar peptade na faʻaaogaina o le faʻataʻitaʻiga ma faʻamamaina e le C18AQQ pou. O le iʻuga, na maua ma mafai ona faʻaaogaina le mea tau faʻatau faʻamoemoe e faʻaaoga nei ma mafai ona faʻaogaina i le suʻesuʻega ma le atinaʻe nei.
Meafaigaluega | Tuufua™masini 2 | |||
Aulautila | 12 G Vaveflash C18 RP Flash Flash Carridge (SHERAMA OLACA, 20 - 45 μm, 100 å (å 5222-2-sp) | 12 G Vaveflash C18AQ RP Flash Cartridge (Shisher Sinica, 20 - 45 μm, 100 å (å 522-sp (AQ) | ||
Galulu | 254 NM, 220 NM | 214 nm | ||
Telefoni feaveaʻi | Sotvent a: vai Socket B: Acetonriori | |||
Tafe fua | 15 ml / min | 20 ml / min | ||
Faʻataʻitaʻiga utaina | 30 mg | |||
Tofa | Taimi (CV) | Sinack B (%) | Taimi (Min) | Sinack B (%) |
0 | 0 | 0 | 4 | |
1.0 | 0 | 1.0 | 4 | |
10.0 | 6 | 7.5 | 18 | |
12.5 | 6 | 13.0 | 18 | |
16.5 | 10 | 14.0 | 22 | |
19.0 | 41 | 15.5 | 22 | |
21.0 | 41 | 18.0 | 38 | |
/ | / | 20.0 | 38 | |
22.0 | 87 | |||
29.0 | 87 |
Faʻaiʻuga ma talanoaga
Ina ia faʻatusatusaina le faʻamamaina o le faʻatinoga mo le porti poptade faʻataʻitaʻiga masani C18 koluma ma C1aq Abnen, na matou faʻaaogaina se C18 Coupin mo le FOWS E pei ona faʻaalia i le Ata 2, ona o le Hydrophobic vaega o le C18 filifili e mafua mai i le maualuga o le fua o le c18 kapoti ua le mafai ona taofia e le soʻotaga masani C18. O le iʻuga, o le faʻataʻitaʻiga e le lelei laʻu taʻaʻi ma faʻamamaina.
Ata 2. O le flash chromatogram o le faʻataʻitaʻiga i se masani C18 pulufana.
Le isi, matou faʻaaogaina le C18AQ koluma mo le FOWS faʻamamaina o le faʻataʻitaʻiga. E pei ona faʻaalia i le Ata 3, na faʻaauau i luga le pept ma le konitia i luga o le koluma ona faʻafetaui ai lea. Na vavaeʻese le oloa o le oloa mai le mama i le poʻo le mea na tupu ma aoina. Ina ua maeʻa Lyophim ona auiliiliga e HPLC, o oloa ua faʻamamaina o loʻo i ai le mama o le 98.2% ma mafai ona faʻaaogaina atili mo isi isi laʻasaga.
Ata 3. O le flash chromatogram o le faʻataʻitaʻiga i luga o le c18aq pulufana.
I le faaiuga, Vaseflash C18AQ RP Flash CartriDid faʻatasi ma le flash chromatography system scanbean™Masini mafai ona ofoina atu se anapogi ma le aoga fofo mo le faʻamamaina o le malosi porla poʻo hydrophilic faʻataʻitaʻiga.
E i ai se faʻasologa o le desemeflash c18aq rp moli laupapa ma eseese faʻataʻitaʻiga mai ia Santai tekonolosi (pei ona faʻaalia i le Lisi 2).
Aitema numera | Koluma tele | Tafe fua (ML / MIN) | Max.Piresture (PSI / BAR) |
SW-5222-004-sp (AQ) | 5.4 g | 5-15 | 400 / 27.5 |
SW-5222-012-Sp (AQ) | 20 g | 10-25 | 400 / 27.5 |
SW-5222-025-sp (AQ) | 33 g | 10-25 | 400 / 27.5 |
SW-5222-040-sp (AQ) | 48 g | 15-30 | 400 / 27.5 |
SW-5222-080-sp (AQ) | 105 g | 25-50 | 350 / 24.0 |
SW-5222-120-sp (AQ) | 155 g | 30-60 | 300 / 20.7 |
SW-5222-220-SP (AQ) | 300 g | 40-80 | 300 / 20.7 |
SW-5222-330-SP (AQ) | 420 g | 40-80 | 250 / 17.2 |
Laulau 2. Vaefateshlash C18AQ RP Flash Ata. Pasing Mea: Lelei-Lelei-Lelei O le COHERLACE C18 (AQ) -Bod Sinica, 20 - 45 μm, 100 å.
Mo nisi faʻamatalaga i auiliiliga auiliili o le databean ™ masini, poʻo le okaina o faʻamatalaga i luga o le dienflash faasologa moli, faʻamolemole asiasi le matou Upega Tafaʻilagi.
Taimi o le Post: Oke-12-2018