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SepaFlash™ HP Series

SepaFlash™ HP Series

SepaFlash™ HP Series Featured Image
  • SepaFlash™ HP Series
  • SepaFlash™ HP Series
  • SepaFlash™ HP Series

Short Description:

HP series flash columns are spin-welded and allow for higher pressure up to 400 psi, the available adapter facilitates offer compatibility with any flash system on the market.

※Fusion series ⸺High resolution, more economical and solvent saving

※Platinum series ⸺ Better consistency and repeatability, low back pressure, high resolution

※Ruby series ⸺ Larger loading volume, less solvent usage, higher degree of separation


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Product Detail

Application

Video

catalogue

Product Introduction

HP series flash columns are spin-welded and allow for higher pressure of up to 400 psi. Available adapter facilitates compatibility with any flash system on the market. This series provides Luer-Lok in and Luer-Lok out flexibility for convenient column stacking. When pre-packed with high-efficiency silica gel (irregular, 25-40 μm, 60 Å; spherical, 20-45 μm, 70 Å), this series presents an outstanding resolution over conventional flash cartridges.

※ Solid, one-piece polypropylene body with thick walls for safety
※ Freely choose irregular silica or spherical silica according to your personal preference
※ Markedly improved resolution and higher sample loading capability
※ Spherical silica provides improved performance without increasing the system backpressure

Benefits of spherical silica gel

For spherical silica gel, strict quality controls from raw material to finished product ensures high lot-to-lot reproducibility and tightly controlled specifications.

※Consistency, reliability, reproducibility
※No contamination, lower backpressure 
※Superior resolution
※Symmetrical peaks with no tailing
※Higher sample loading capacity

spherical

HP Fusion Series -- High resolution, more economical and solvent saving

High-efficiency irregular silica, 25−40 µm, 60 Å (surface area 500 m2 /g, pH 6.5–7.5, loading capacity 0.1–15%)

Item Number Column Size Sample Size(g) Flow Rate(mL/min) Cartridge Length (cm) Cartridge ID (mm) Max. Pressure(psi/bar) Quantity per Box
Small Large
SW-5102-004 4g 4mg-0.6g 15–30 113.8 12.4 400/27.5 24 120
SW-5102-012 12g 12mg-1.8g 25–50 134.8 21.4 400/27.5 24 108
SW-5102-025 25g 25mg-3.8g 25–50 184 21.4 400/27.5 18 72
SW-5102-040 40g 40mg-6.0g 30–60 184.4 26.7 400/27.5 12 48
SW-5102-080 80g 80mg-12g 40–80 257.4 31.2 350/24.0 10 20
SW-5102-120 120g 120mg-18g 45–90 261.5 38.6 300/20.7 8 16
SW-5102-220 220g 220mg-33g 60–120 223.5 61.4 300/20.7 4 8
SW-5102-330 330g 330mg-50g 60–120 280.2 61.4 250/17.2 3 6
SW-5102-800 800g 800mg-120g 50-100 382.9 78.2 100/6.9 3 -
SW-5102-1600 1600g 1.6g-240g 50-100 432.4 103.8 100/6.9 2 -
SW-5102-3000 3kg 3g-450g 50-100 509.5 127.5 100/6.9 1 -
SW-5102-5000 5kg 5g-750g 50-100 770 127.5 100/6.9 1 -
SW-5102-010K 10kg 10g-1.5kg 50-100 850 172.5 100/6.9 1 -

※ Compatible with all flash chromatography systems on the market.

Application of SepaFlash™ HP Fusion Series

Purification of compounds that are difficult to separate by flash chromatography (ΔRf ≤ 0.2 between spots on TLC) often results in additional steps such as subsequent purification by preparative scale HPLC. It is possible to reduce the amount of additional work required for purification by simply stacking several prepacked SepaFlash™ columns end to end on a flash chromatography system.

In liquid chromatography, chemical species are separated on the basis of their differences in velocity as they move through the column. Increasing column length can significantly increase resolution. By stacking columns end to end the length to diameter (L to D) ratio is increased so that no major changes to the media and solvent system are necessary. Often this increased L to D is sufficient to provide successful separation of difficult mixtures due to close compounds retention time that is not obtained on a single column. The data illustrates the linear relationship between the resolution and overall column length.

HP1
HP

HP Platinum Series ⸺ Consistency, repeatability, low back pressure, high resolution, solvent saving

UltraPure spherical silica, 40−75 µm, 70 Å (surface area 500 m2 /g, pH 6.0–8.0, loading capacity 0.1–10%)

Item Number Column Size Sample Size(g) Flow Rate(mL/min) Cartridge Length (cm) Cartridge ID (mm) Max. Pressure(psi/bar) Quantity per Box
Small Large
SW-2101-004-SP 4g 4mg–0.4g 15–40 113.8 12.4 400/27.5 24 120
SW-2101-012-SP 12g 12mg–1.2g 30–60 134.8 21.4 400/27.5 24 108
SW-2101-025-SP 25g 25mg–2.5g 30–60 184 21.4 400/27.5 18 72
SW-2101-040-SP 40g 40mg–4.0g 40–70 184.4 26.7 400/27.5 12 48
SW-2101-080-SP 80g 80mg–8.0g 50–100 257.4 31.2 350/24.0 10 20
SW-2101-120-SP 120g 120mg–12g 60–150 261.5 38.6 300/20.7 8 16
SW-2101-220-SP 220g 220mg–22g 80–220 223.5 61.4 300/20.7 4 8
SW-2101-330-SP 330g 330mg–33g 80–220 280.2 61.4 250/17.2 3 6
SW-2101-800-SP 800g 800mg-80g 50-100 382.9 78.2 100/6.9 3 -
SW-2101-1600-SP 1600g 1.6g-160g 50-100 432.4 103.8 100/6.9 2 -
SW-2101-3000-SP 3kg 3g-300g 50-100 509.5 127.5 100/6.9 1 -
SW-2101-5000-SP 5kg 5g-500g 50-100 770 127.5 100/6.9 1 -
SW-2101-010K-SP 10kg 10g-1kg 50-100 850 172.5 100/6.9 1 -

※Compatible with all flash chromatography systems on the market.

High-efficiency spherical silica, 20−45 µm, 70 Å (surface area 500 m2 /g, pH 6.0–8.0, loading capacity 0.1–15%)

Item Number Column Size Sample Size(g) Flow Rate(mL/min) Cartridge Length (cm) Cartridge ID (mm) Max. Pressure(psi/bar) Quantity per Box
Small Large
SW-2102-004-SP 4g 4mg–0.6g 15–30 113.8 12.4 400/27.5 24 120
SW-2102-012-SP 12g 12mg–1.8g 25-50 134.8 21.4 400/27.5 24 108
SW-2102-025-SP 25g 25mg–3.8g 25-50 184 21.4 400/27.5 18 72
SW-2102-040-SP 40g 40mg–6.0g 30–60 184.4 26.7 400/27.5 12 48
SW-2102-080-SP 80g 80mg–12g 40–80 257.4 31.2 350/24.0 10 20
SW-2102-120-SP 120g 120mg–18g 45–90 261.5 38.6 300/20.7 8 16
SW-2102-220-SP 220g 220mg–33g 60–120 223.5 61.4 300/20.7 4 8
SW-2102-330-SP 330g 330mg–50g 60–120 280.2 61.4 250/17.2 3 6
SW-2102-800-SP 800g 800mg-120g 50-100 382.9 78.2 100/6.9 3 -
SW-2102-1600-SP 1600g 1.6g-240g 50-100 432.4 103.8 100/6.9 2 -
SW-2102-3000-SP 3kg 3g-450g 50-100 509.5 127.5 100/6.9 1 -
SW-2102-5000-SP 5kg 5g-750g 50-100 770 127.5 100/6.9 1 -
SW-2102-010K-SP 10kg 10g-1.5kg 50-100 850 172.5 100/6.9 1 -

※Compatible with all flash chromatography systems on the market.

HP Ruby Series ⸺Larger loading volume, less solvent usage, higher degree of separation

High capacity spherical silica, 50 μm, 54 Å (surface area 700 m2/g, pH 6.0‒8.0, loading capacity 0.1‒30%)

Item Number Column Size Sample Size(g) Flow Rate(mL/min) Cartridge Length (cm) Cartridge ID (mm) Max. Pressure(psi/bar) Quantity per Box
Small Large
SW-2101-004-SP(H) 4g 4mg–1.2g 15–30 113.8 12.4 400/27.5 24 120
SW-2101-012-SP(H) 12g 12mg–3.6g 25-50 134.8 21.4 400/27.5 24 108
SW-2101-025-SP(H) 25g 25mg–7.5g 25-50 184 21.4 400/27.5 18 72
SW-2101-040-SP(H) 40g 40mg–12g 30-60 184.4 26.7 400/27.5 12 48
SW-2101-080-SP(H) 80g 80mg–24g 40-80 257.4 31.2 350/24.0 10 20
SW-2101-120-SP(H) 120g 120mg–36g 45-90 261.5 38.6 300/20.7 8 16
SW-2101-220-SP(H) 220g 220mg–66g 60-120 223.5 61.4 300/20.7 4 8
SW-2101-330-SP(H) 330g 330mg–99g 60-120 280.2 61.4 250/17.2 3 6
SW-2101-800-SP(H) 800g 800mg-240g 50-100 382.9 78.2 100/6.9 3 -
SW-2101-1600-SP(H) 1600g 1.6g-480g 50-100 432.4 103.8 100/6.9 2 -
SW-2101-3000-SP(H) 3kg 3g-900g 50-100 509.5 127.5 100/6.9 1 -
SW-2101-5000-SP(H) 5kg 5g-1.5kg 50-100 770 127.5 100/6.9 1 -
SW-2101-010K-SP(H) 10kg 10g-3kg 50-100 850 172.5 100/6.9 1 -

※Compatible with all flash chromatography systems on the market.

High-capacity spherical silica, 25 µm, 50 Å (surface area 700 m2 /g, pH 5.0–8.0, loading capacity 0.1–30%)

Item Number Column Size Sample Size(g) Flow Rate(mL/min) Cartridge Length (cm) Cartridge ID (mm) Max. Pressure(psi/bar) Quantity per Box
Small Large
SW-2102-004-SP(H) 4g 4mg–1.2g 15–30 113.8 12.4 400/27.5 24 120
SW-2102-012-SP(H) 12g 12mg–3.6g 25-50 134.8 21.4 400/27.5 24 108
SW-2102-025-SP(H) 25g 25mg–7.5g 25-50 184 21.4 400/27.5 18 72
SW-2102-040-SP(H) 40g 40mg–12g 30-60 184.4 26.7 400/27.5 12 48
SW-2102-080-SP(H) 80g 80mg–24g 40-80 257.4 31.2 350/24.0 10 20
SW-2102-120-SP(H) 120g 120mg–36g 45-90 261.5 38.6 300/20.7 8 16
SW-2102-220-SP(H) 220g 220mg–66g 60-120 223.5 61.4 300/20.7 4 8
SW-2102-330-SP(H) 330g 330mg–99g 60-120 280.2 61.4 250/17.2 3 6
SW-2102-800-SP(H) 800g 800mg-240g 50-100 382.9 78.2 100/6.9 3 -
SW-2102-1600-SP(H) 1600g 1.6g-480g 50-100 432.4 103.8 100/6.9 2 -
SW-2102-3000-SP(H) 3kg 3g-900g 50-100 509.5 127.5 100/6.9 1 -
SW-2102-5000-SP(H) 5kg 5g-1.5kg 50-100 770 127.5 100/6.9 1 -
SW-2102-010K-SP(H) 10kg 10g-3kg 50-100 850 172.5 100/6.9 1 -

※Compatible with all flash chromatography systems on the market.

High-capacity spherical silica, 15 µm, 50 Å  (surface area 700 m2 /g, pH 5.0–8.0, loading capacity 0.1–30%)

Item Number Column Size Sample Size(g) Flow Rate(mL/min) Cartridge Length (cm) Cartridge ID (mm) Max. Pressure(psi/bar) Quantity per Box
Small Large
SW-2103-004-SP(H) 4 g 4 mg–1.2 g 10–15 113.8 12.4 400/27.5 24 120
SW-2103-012-SP(H) 12 g 12 mg–3.6 g 15–20 134.8 21.4 400/27.5 24 108
SW-2103-025-SP(H) 25 g 25 mg–7.5 g 15–20 184 21.4 400/27.5 18 72
SW-2103-040-SP(H) 40 g 40 mg–12 g 20–30 184.4 26.7 400/27.5 12 48
SW-2103-080-SP(H) 80 g 80 mg–24 g 30–40 257.4 31.2 350/24.0 10 20
SW-2103-120-SP(H) 120 g 120 mg–36 g 35–45 261.5 38.6 300/20.7 8 16
SW-2103-220-SP(H) 220 g 220 mg–66 g 50–65 223.5 61.4 300/20.7 4 8
SW-2103-330-SP(H) 330 g 330 mg–99 g 50–65 280.2 61.4 250/17.2 3 6

※ Compatible with all flash chromatography systems on the market.

Application of SepaFlash™ HP Ruby Series

Double Loading Capacity of SepaFlash™ Cartridges pre-packed with high-capacity silica gel (700 m2 /g for 50 Å pore size) delivers the highest purification performance. The high-capacity 25 µm or 15 µm spherical silica has 40% higher surface area, doubling the loading capacity of lower surface area silica. Santai evaluated the performance of the SepaFlash™ columns compared to two well-known brands. Results show SepaFlash™ outperforms the competitors.

HP3
HP4
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