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Porogaramu R & D Centre
Intangiriro
Peptide ni ikigo gigizwe na acide amino, buri kimwe muricyo gifite imitungo idasanzwe yumubiri na shimi bitewe nuburyo butandukanye nibikorwa bya aside aside bigize aside bigize urukurikirane. Hamwe niterambere ryicyiciro gikomeye cyicyiciro cya Synthesis, synthesique ya buri gihe iratera imbere. Ariko, kubera ibigize uruhare rugoye yabonetse na synthesi yicyiciro gikomeye, ibicuruzwa byanyuma bigomba kwezwa nuburyo bwo gutandukana. Uburyo busanzwe bwo kwezwa bwo kwegeranya muri ion Guhana Chromatografiya (IEC) hamwe nibikoresho byogurika bya mopromatografi, nibindi <1 KDA <1 KDAI) byatangajwe na Santai Tekinoroji, aho ikwirakwizwa rya C18 ryakoreshejwe kugirango ryegereje kuri thymopentin (TP-5) hamwe nibicuruzwa bisabwa nibisabwa byabonetse.
Igishushanyo 1. 20 aside isanzwe ya Amine (itanga kuri www.bachem.com).
Hariho ubwoko 20 bwa aside amine ikunze kugaragara mu bigize urwenya. Aba aside amine barashobora kugabana mumatsinda akurikira bakurikije imitungo yabo n imitungo yabo: bitari (hydrophobic), polar (polar (acide), nkuko bigaragara ku gishushanyo 1). Mu rutonde rwa Peptade, niba aside amine ikubiyemo urutonde rutandukanye cyane (nkuko bigaragara ku ibara ryijimye mu gishushanyo 1), nka seterine, igifuniko, iki gihe cyo kuringaniza gishobora kugira polarite ikomeye kandi kigakundwa cyane mumazi. Mugihe cyo kweza kuri polar ikomeye ya Poplade yahinduwe-icyiciro cya chromatography, icyiciro cyicyiciro cya hydrophomike kizahinduka (reba ibyanditswe mbere na tekinoroji ya Santai, Aq yahinduwe ninkingi yicyiciro hamwe na porogaramu). Ugereranije n'imiyoboro isanzwe ya C18, inkingi za C18AQ zibereye cyane gusukura ingero zikomeye cyangwa hydrophilique. Muri iyi nyandiko, peptide ikomeye yakoreshejwe nkicyitegererezo kandi isukurwa ninkingi ya C18AQ. Nkigisubizo, ibicuruzwa bigamije gusaba ibisabwa kandi birashobora gukoreshwa mubushakashatsi no guteza imbere ubushakashatsi bukurikira.
| Igikoresho | Sepaben™imashini 2 | |||
| Amakarito | 12 G DissFlash C18 Rp Flash Cartridge (SPERICACT SILICA, 20 - 45 μm, 100 å, gutumiza nune: sw-5222-012-sp) | 12 G DissFlash C18AQ Rp Flash Cartridge (SPERICACT SILICA, 20 - 45 μM, 100 å, SW-5222-012-SP (AQ)) | ||
| Uburebure | 254 nm, 220 nm | 214 nm | ||
| Icyiciro cya mobile | Gukemura a: amazi Yamazaki B: Acetonitrile | |||
| Igipimo | 15 ml / min | 20 ml / min | ||
| Icyitegererezo | 30 mg | |||
| Gradient | Igihe (CV) | Yamazaki B (%) | Igihe (Min) | Yamazaki B (%) |
| 0 | 0 | 0 | 4 | |
| 1.0 | 0 | 1.0 | 4 | |
| 10.0 | 6 | 7.5 | 18 | |
| 12.5 | 6 | 13.0 | 18 | |
| 16.5 | 10 | 14.0 | 22 | |
| 19.0 | 41 | 15.5 | 22 | |
| 21.0 | 41 | 18.0 | 38 | |
| / | / | 20.0 | 38 | |
| 22.0 | 87 | |||
| 29.0 | 87 | |||
Ibisubizo n'ibiganiro
Kugereranya imikorere yo kwezwa kuri Polar Perpede hagati yinkingi ya C18 hamwe na C18AQ, twakoresheje inkingi isanzwe ya C18 yo kwezwa kuri flash nkicyitegererezo nkicyitegererezo. Nkuko bigaragara ku gishushanyo cya 2, kubera icyiciro cya hydrophobike cyo gusenyuka kw'iminyururu ya C18 zatewe n'ikigereranyo kinini cya Cartrid kandi cyavanywe mu cyiciro cya mobile. Nkigisubizo, icyitegererezo nticyatandukanijwe neza kandi cyezwa.
Igishushanyo cya 2. Flash chromatogram yicyitegererezo kuri cartridge isanzwe ya c18.
Ibikurikira, twakoresheje C18AQ inkingi ya flash yerekana icyitegererezo. Nkuko bigaragara ku gishushanyo cya 3, peptade yagumishijwe neza ku nkingi hanyuma yataye. Ibicuruzwa byateganijwe byatandukanijwe numwanda mubisatsi bibisi kandi byegeranijwe. Nyuma ya Lynophilisation hanyuma isesengurwa na HPLC, ibicuruzwa byera bifite isuku ya 98.2% kandi birashobora gukomeza gutondekanya intambwe itaha.
Igishushanyo cya 3. Flash chromatogram yumutego kuri cartridge ya C18AQ.
Mu gusoza, Gutandukanya C18AQ RP Flash Cartridge yahujwe na flaromatgrafiya sisitemu sepabean™Imashini irashobora gutanga igisubizo cyihuse kandi cyiza cyo kwezwa kwicyitegererezo cya polar cyangwa hydrophilic.
Hano hari urukurikirane rwa Startflash C18AQ rp flash cartridges hamwe nibisobanuro bitandukanye kuva tekinoroji ya santai (nkuko bigaragara mu mbonerahamwe ya 2).
| Umubare w'ikintu | Ingano y'inkingi | Igipimo (ML / Min) | Max.kwishimira (PSI / BAR) |
| SW-5222-004-SP (AQ) | 5.4 g | 5-15 | 400/1 27.5 |
| SW-5222-012-SP (AQ) | 20 g | 10-25 | 400/1 27.5 |
| SW-5222-025-SP (AQ) | 33 g | 10-25 | 400/1 27.5 |
| Sw-5222-040-SP (AQ) | 48 g | 15-30 | 400/1 27.5 |
| Sw-5222-080-sp (aq) | 105 g | 25-50 | 350 / 24.0 |
| Sw-5222-120-SP (AQ) | 155 g | 30-60 | 300 / 20.7 |
| SW-5222-220-SP (AQ) | 300 g | 40-80 | 300 / 20.7 |
| SW-5222-330-SP (AQ) | 420 G. | 40-80 | 250 / 17.2 |
Imbonerahamwe ya 2. Gutandukanya C18AQ RP FL Flash Cartridges. Ibikoresho byo gupakira: Gukora neza-Imikorere ya Spherical C18 (AQ) -Yambere Silica, 20 - 45 μm, 100 å.
Ukeneye ibisobanuro birambuye kubisobanuro birambuye bya sevabean ™ imashini ya sepabean ™ cyangwa amakuru yo gutumiza kuri DissFlash Urukurikirane rwa Flash Cartridges, nyamuneka sura urubuga rwacu.
Kohereza Igihe: Ukwakira-12-2018