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Nigute ushobora guhuza inkingi za iLOK yubusa kuri sisitemu ya Biotage？
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Silika ikora irashonga mumazi?
Oya, silika ifunze amaherezo ntishobora gukemuka mubisanzwe ikoreshwa bisanzwe.
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Ni izihe ngingo zo kwitondera gukoresha C18 flash inkingi?
Kugirango usukure neza hamwe na C18 flash inkingi, nyamuneka kurikiza izi ntambwe:
① Shyira inkingi hamwe na 100% yumuti ukomeye (organic) solvent kuri CV 10 - 20 (ingano yinkingi), mubisanzwe methanol cyangwa acetonitrile.
② Koresha inkingi hamwe na 50% ikomeye + 50% y'amazi (niba hakenewe inyongeramusaruro, shyiramo) kubindi CV 3 - 5.
③ Koresha inkingi hamwe nintangiriro ya gradient ya 3 - 5 CV. -
Niki gihuza flash nini nini?
Kubunini bwinkingi hagati ya 4g na 330g, umuhuza wa Luer usanzwe ukoreshwa muribi flash. Kubunini bwinkingi ya 800g, 1600g na 3000g, hiyongereyeho adapteri ihuza imashini kugirango ushireho izo nkingi nini za flash kuri sisitemu ya flash chromatografiya. Nyamuneka reba inyandiko Santai Adapter Kit ya 800g, 1600g, 3kg Flash Inkingi kugirango ubone ibisobanuro birambuye.
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Niba karitsiye ya silika ishobora gutorwa na methanol cyangwa ntayo?
Ku nkingi isanzwe yicyiciro, birasabwa gukoresha icyiciro kigendanwa aho igipimo cya methanol kitarenga 25%.
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Nuwuhe mipaka yo gukoresha amashanyarazi nka DMSO, DMF?
Mubisanzwe, birasabwa gukoresha icyiciro kigendanwa aho igipimo cyumuti wa polar kitarenga 5%. Umuyoboro wa polar urimo DMSO, DMF, THF, TEA nibindi
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Ibisubizo byo gupakira icyitegererezo gikomeye?
Icyitegererezo gikomeye cyo gupakira ni tekinike yingirakamaro yo gupakira icyitegererezo kugirango gisukurwe ku nkingi, cyane cyane kuburugero rwibisubizo bike. Muri iki kibazo, iLOK flash cartridge ni amahitamo meza cyane.
Mubisanzwe, icyitegererezo gishonga mumashanyarazi akwiye kandi kikanashyirwa kumurongo ukomeye wa adsorbant ushobora kuba nkuwakoreshejwe mumashanyarazi, harimo isi ya diatomaceous cyangwa silika cyangwa ibindi bikoresho. Nyuma yo kuvanaho / guhumeka byumuti usigaye, adsorbent ishyirwa hejuru yinkingi yuzuye igice cyangwa muri karitsiye yuzuye yuzuye. Kubindi bisobanuro birambuye, nyamuneka reba inyandiko iLOK-SL Cartridge Umukoresha uyobora ibisobanuro birambuye. -
Nubuhe buryo bwo gupima ingano yinkingi ya flash inkingi?
Ingano yinkingi ihwanye nubunini bwapfuye (VM) mugihe wirengagije ingano yinyongera muri tubing ihuza inkingi na inshinge na detector.
Igihe cyapfuye (tM) nigihe gikenewe cyo gukuraho ikintu kitagabanijwe.
Ingano yapfuye (VM) nubunini bwicyiciro kigendanwa gisabwa kugirango ikureho ikintu kitagabanijwe. Ingano yapfuye irashobora kubarwa nuburinganire bukurikira: VM = F0 * tM.
Muburinganire bwavuzwe haruguru, F0 nigipimo cyo gutembera kwicyiciro cya mobile.
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Ese silika ikora irashonga muri methanol cyangwa ikindi kintu cyose gisanzwe kama kama?
Oya, silika ifunze amaherezo ntishobora gukemuka mubisanzwe ikoreshwa bisanzwe.
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Niba silika flash cartridge ishobora gukoreshwa inshuro nyinshi cyangwa ntayo?
Amashanyarazi ya silika ya silika arashobora gukoreshwa kandi kugirango akoreshwe rimwe, ariko hamwe no gufata neza, amakarito ya silika arashobora kongera gukoreshwa atitanze kubikorwa.
Kugirango ukoreshwe, inkingi ya silika ya silika igomba gukama gusa numwuka uhumeka cyangwa ukajugunywa hamwe ukabikwa muri isopropanol. -
Ni ubuhe buryo bukwiye bwo kubungabunga C18 flash cartridge?
Kubika neza bizemerera C18 flash inkingi kongera gukoreshwa:
• Ntuzigere wemerera inkingi gukama nyuma yo gukoresha.
• Kuraho ibintu byose byahinduye ibinyabuzima uhinduranya inkingi hamwe na 80% methanol cyangwa acetonitrile mumazi kuri CV 3 - 5.
• Bika inkingi mumashanyarazi yavuzwe haruguru hamwe na fitingi zanyuma. -
Ibibazo bijyanye ningaruka zumuriro muburyo bwa pre-equilibrium kuri flash inkingi?
Kubunini bunini bwinkingi ziri hejuru ya 220g, ingaruka yubushyuhe iragaragara mugikorwa cya pre-equilibrium. Birasabwa gushyiraho umuvuduko wa 50-60% yikigereranyo cyogutemba mugikorwa mbere yo kuringaniza kugirango wirinde ingaruka zubushyuhe bugaragara.
Ingaruka yumuriro ivanze yumuti iragaragara cyane kuruta umusemburo umwe. Fata sisitemu ya cyclohexane / Ethyl acetate nkurugero, birasabwa ko ukoresha 100% cyclohexane muburyo bwambere bwo kuringaniza. Iyo pre-equilibration irangiye, igeragezwa ryo gutandukana rishobora gukorwa ukurikije sisitemu yateganijwe.
