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Nigute ushobora guhuza inkingi zubusa kuri sisitemu ya biotage?
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Ese imikorere ya Silica yashonga mumazi?
Oya, iherezo-ryafashwe Silica ihumeka mubintu byose bikoreshwa mubi.
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Ni izihe ngingo zo kwitabwaho ukoresheje C18 flash inkingi?
Kubisumbaza neza hamwe na C18 flash inkingi, nyamuneka kurikiza izi ntambwe:
① Kuzunguza inkingi hamwe 100% byimbaraga zikomeye (kama) cvs 10 - 20 cvs (ingano yinkingi), mubisanzwe methanol cyangwa acetonitrile.
② Kuzunguza inkingi hamwe na 50% + 50% areque (niba inyongeramusaruro zisabwa, shyiramo) kurindi 3 - 5.
③ Fungura inkingi hamwe nibihe byambere kuri 3 - 5. -
Niki cector yinkingi nini?
Kubunini bwinkingi hagati ya 4g na 330g, bisanzwe luer guhuza bikoreshwa muri iyi nkingi za flash. Kubunini bwinkingi ya 800g, 1600g na 3000g, Adaptor yinyongera yakazi igomba gukoreshwa mu gushinga iyi nkingi nini za flash chromatography. Nyamuneka reba inyandiko ya Santai adapt ya 800g, 1600g, 3kg inkingi kubisobanuro birambuye.
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Niba Carrid Cartridge ishobora gusohora na methanol cyangwa sibyo?
Kubice bisanzwe byigipanga, birasabwa gukoresha icyiciro cya mobile aho igipimo cya methanol kitarenze 25%.
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Ni ubuhe buryo bwo gukoresha Polar Prevents nka DMSO, DMF?
Mubisanzwe, birasabwa gukoresha icyiciro cya mobile aho igipimo cya polar polan kitarenze 5%. Polar Devents ikubiyemo DMSO, DMF, THF, icyayi nibindi.
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Ibisubizo byicyitegererezo gikomeye?
Icyitegererezo gikomeye cyo gupakira nubuhanga bwingirakamaro kugirango ushireho icyitegererezo kugirango usukure ku nkingi, cyane cyane ku byitegererezo byo kwigunga hasi. Muri iki kibazo, Ilok Flash Cartridge ni amahitamo akwiye.
Mubisanzwe, icyitegererezo gishonga muburyo bukwiye kandi gihuzwa na adsorbant ikomeye ishobora kuba imwe mubice bya flash, harimo na diatomaceous cyangwa silica cyangwa ibindi bikoresho. Nyuma yo gukuraho / guhumeka ibintu bisigaye, adsorbent ishyirwa hejuru yinkingi yuzuye igice cyangwa mubusa bwa karitsiye. Kubindi bisobanuro birambuye, nyamuneka reba inyandiko Ilok-Sl Cartridge ukoresha ibisobanuro birambuye. -
Nubuhe buryo bwo kwipimisha bwinkingi ingano yinkingi ya flash?
Umubumbe w'inkingi ugereranyije nubunini bwabapfuye (VM) mugihe wirengagije amajwi yinyongera muburyo bwo guhuza inkingi hamwe na injiji na detector.
Igihe cyapfuye (TM) nigihe gisabwa kugirango umuntu yibohoze.
Umubumbe wapfuye (VM) nijwi ryicyiciro cya mobile gisabwa kugirango umuntu yibohoze. Umubumbe wapfuye urashobora kubarwa ukurikije ikigereranyo gikurikira: Vm = F0 * TM.
Mugereranya haruguru, F0 ni igipimo cyuruzi cyicyiciro cya mobile.
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Ese imikorere ya silica ishonga muri methanol cyangwa ikindi kintu cyose gisanzwe?
Oya, iherezo-ryafashwe Silica ihumeka mubintu byose bikoreshwa mubi.
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Niba silica Flash Cartridge irashobora gukoreshwa inshuro nyinshi cyangwa ntabwo?
Inkingi ya silica flash ikorwa kandi igakoreshwa rimwe, ahubwo ifite uburyo bwiza, Carrida ya Silica irashobora gukoreshwa nta kwigomwa.
Kugirango dusubizwe, inkingi ya Silica Flam igomba gukama gusa umwuka ufunzwe cyangwa usunika kandi ibika muri IsOpropanol. -
Nibihe bintu byo kubungabunga neza kuri C18 Flash Cartridge?
Ububiko bukwiye buzemerera C18 inkingi za flash zigomba gukoreshwa:
• Ntuzigere wemerera inkingi yumye nyuma yo gukoresha.
• Kuraho impinduka zose kama uhindagurika inkingi hamwe na 80% methanol cyangwa acetonitrile mumazi ya 3 - 5.
• Bika inkingi muri iki cyavuzwe haruguru kugasumba cyane hamwe na fithings. -
Ibibazo bijyanye nimitingi munzira yabanjirije pre-kuri flash inkingi?
Kubiringano binini binini hejuru ya 220g, ingaruka zubushyuhe ziragaragara mubikorwa bya pre-ofiribrium. Birasabwa gushiraho igipimo cyurugendo kuri 50-60% byigipimo cyuruganda rutabwe munzira ya mbere yo kuringaniza kugirango wirinde ingaruka zubushyuhe.
Ingaruka zumuriro zivanze cyane ziragaragara kuruta soling imwe. Fata sisitemu ya sorvelvex / Ethyl atera nkurugero, irasabwa ko gukoresha ibikatsi 100% mugihe cyambere cyuzuye. Iyo habaye uburinganire bwuzuye, ubushakashatsi bwo gutandukana bushobora gukorwa hakurikijwe gahunda ya Preset.
