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Chiyambi
Ion kusinthitsa ma chromatography (IEC) ndi njira ya chromatographic yomwe nthawi zambiri imagwiritsidwa ntchito kupatukana ndikuyeretsa zinthu zomwe zimaperekedwa mu Ionic Fomu. Malinga ndi masitepe osiyanasiyana a ions, iec ikhoza kugawidwa m'mitundu iwiri, kusinthana kwa chromatography ndi anyezi kusinthana kwa chromatography. Mu cur Kusinthanitsa chromatography, magulu acidic amalumikizidwa kumtunda kwa mafayilo olekanitsa. Mwachitsanzo, sulfonic acid (-So3h) ndi gulu lomwe limagwiritsidwa ntchito posinthana ndi ma cent (scx), omwe amasungunuka H + ndi gulu lina la ADSORB ina mu yankho. Mu Anion kusinthana kwa ma chromatography, magulu am'magulu a alkaline amalumikizidwa kumtunda kwa mafayilo olekanitsa. Mwachitsanzo, Amine odziwika (-NR, komwe r ndi RIPHOCOCAROB) nthawi zambiri imagwiritsidwa ntchito posinthana mwamphamvu anion (SAX), ndipo
Mwazinthu zachilengedwe, Flavonoids akopa chidwi cha ofufuza chifukwa chogwira nawo ntchito popewa komanso kuchiza matenda amtima. Popeza mamolekyu a flavonoid ndi acidic chifukwa cha magulu a phenolic hydroxyl, ion kusinthana ndi mankhwala ena kuwonjezera pa gawo wamba kapena kukonza gawo la acidication. Mu flambatography, makanema omwe amagwiritsidwa ntchito polekanitsa a ion kusinthitsa ndi silika gel pomwe madera osinthana ndi ion. Zogwiritsidwa ntchito kwambiri zogwiritsidwa ntchito kwambiri ku matope a chromatography ndi scx (nthawi zambiri sulfonic acid) ndi Sax (nthawi zambiri aminathertery). M'makalata omwe adasindikizidwa kale ndi mutu wakuti "Kugwiritsa ntchito kwa OlentFalash Mitundu Yosiyanasiyana Kusintha kwa Alkaline. Mu positi iyi, zosakaniza za miyezo yandale komanso acidic idagwiritsidwa ntchito ngati chitsanzo kuti mufufuze kugwiritsidwa ntchito kwa zinyama ya acidific.
Gawo Loyesera
Chithunzi 1. Chithunzi chojambulidwa cha gawo la gawo lolumikizidwa mpaka pamtunda wa kupatukana kwa Sax.
Mu positi iyi, mzati wa Sax adadzaza ndi Amine Amine wolumikizidwa silika yemwe amagwiritsidwa ntchito (monga akuwonetsera pa Chithunzi 1). Kusakaniza kwa chromone ndi 2,4-dihhyroxybenzoic acid adagwiritsidwa ntchito ngati zitsanzo zoyeretsedwa (monga zikuwonekera pa Chithunzi 2). Kusakaniza kunasungunuka ku Methanol ndikunyamula ku Flatridge yokhala ndi invor. Kukhazikitsa kwa kuyeretsa kwa Flash kumalembedwa pagome 1.
Chithunzi 2. Kapangidwe ka mankhwala awiriwo mu zitsanzo zosakaniza.
| Chipangizo | Sepiban ™ Machine t | |||||
| Makatoni | 4 g clepolash Standard Getridge Cartridge (silika yosakhazikika, 40 - 63 μ, dongosolo la oda: Sh10101-0004) | 4 g clempulash wogwirizanitsa Sax Squatridge (silika wosakhazikika, 40 - 63 Å, nambala ya oda: SW - IR) | ||||
| Pukhuta | 254 nm (kudziwika), 280 nm (kuwunikira) | |||||
| Gawo lam'manja | Zosungunulira a: n-hexane | |||||
| Solvent b: ethyl acetate | ||||||
| Kutentha | 30 ml / min | 20 ml / min | ||||
| Zitsanzo Zotsegulira | 20 mg (chisakanizo cha gulu la a ndi gawo b) | |||||
| Nyumba | Nthawi (CV) | Solvent b (%) | Nthawi (CV) | Solvent b (%) | ||
| 0 | 0 | 0 | 0 | |||
| 1.7 | 12 | 14 | 100 | |||
| 3.7 | 12 | / | / | |||
| 16 | 100 | / | / | |||
| 18 | 100 | / | / | |||
Zotsatira ndi zokambirana
Choyamba, kusakaniza kwachitsanzo kunalekanitsidwa ndi gawo labwinobwino la cartridge chisananyamuke ndi silika nthawi zonse. Monga zikuwonekera pa Chithunzi 3, zinthu ziwirizo mu sample zidayikidwa pa cartridge wina pambuyo pake. Kenako, katoni wa Sax Flush adagwiritsidwa ntchito pakuyeretsa kwa zitsanzo. Monga zikuwonekera pa Chithunzi 4, gulu la acidic B lidasungidwa kwathunthu pa cartridge ya Sax. The osalowerera ndale aja adadetsedwa pang'onopang'ono pa cartridge ndi echi.
Chithunzi 3. Flack Chromatogram ya zitsanzo pa cartridge wamba.
Chithunzi 4. The Flack Chromatogram ya zitsanzo pa cartridge.
Kuyerekeza Chithunzi 3 ndi Chithunzi 4, Chigawocho chiri ndi mawonekedwe osagwirizana ndi matope awiri osiyana. Kuti mutsimikizire ngati nsonga yazofananirayo ikugwirizana ndi gawo, titha kugwiritsa ntchito njira yonse yolumikizira yomwe imapangidwa mu pulogalamu ya Speaban ™ makina. Tsegulani deta yoyeserera yapatali katatu, kukoka kwa mzere wa zisonyezo pa nthawi yomwe axis (CV) mu chromatogram mpaka pamlingo wambiri wa mfundo ziwirizi (monga momwe chithunzi 6). Kuyerekezera chidziwitso chonse cha kudzipatula kwapamenezi, chiani chigululi cha mayamwidwe muyeso muyeso awiri. Chifukwa cha gawo la chinthucho chiri ndi mawonekedwe osavomerezeka pa matotoni awiri osiyana, zimaganiziridwa kuti pali zodetsazo mu gulu la cartridge yokhazikika ndi katoni wa Sax. Chifukwa chake, kusintha kwa eling ndi kosiyana ndi gawo la makatoni awiriwa, zomwe zimapangitsa chinsinsi chosagwirizana ndi ma chromatogram.
Chithunzi 5. Kupukutira kwathunthu kwa gawo la gawo la gawo la gawo la magawo.
Chithunzi 6. Kupukutira kwathunthu kwa gawo la SAX CARRIDGE.
Ngati chinthu chandamale chosonkhanitsidwa ndi gawo la kusalowererapo a, omwe akuyeretsa amatha kumaliza kugwiritsa ntchito makatoni a Sax a startridge pambuyo pake. Kumbali inayo, ngati chinthu chandamale choyenera kusonkhanitsidwa ndi gawo la acidic itangokhazikitsidwa pang'ono poyesera muyeso wa Sax, sinthanitsani gawo la ma methanol omwe ali ndi 5% acetic acid. Ma ionstiate ace mu gawo la foni azipikisana ndi gulu la Amine Amine Amine a Amine Cartridge, potero, kuwira chigawo cha B kuchokera ku Cartridge kuti apeze chinthu chandamale. Chromatogram ya zitsanzo zolekanitsidwa mu ma ion kusinthidwa kuwonetsedwa mu Chithunzi 7.
Chithunzi 7. Flash Chromatogram ya chigawo cha B cholumikizidwa mu mawonekedwe a ion Cartridge.
Pomaliza, chitsanzo cha acidic kapena chosalowerera chikhoza kutsukidwa mwachangu ndi sax cartridge chophatikizidwa ndi kabatizo wamba pagawo loyeretsa. Kuphatikiza apo, mothandizidwa ndi kuwunika kwathunthu kwa Highleng komwe adapangidwa ndi mapulogalamu a Sepleaban ™
| Nambala ya chinthu | Kukula kwa mzere | Kutentha (ml / min) | Max.Press (PSI / Bar) |
| SW-5001-004-ir | 5.9 g | 10-20 | 400 / 27.5 |
| SW-5001-012-ir | 23 g | 15-30 | 400 / 27.5 |
| SW-5001-025-ir | 38 g | 15-30 | 400 / 27.5 |
| SW-5001-040-ir | 55 g | 20-40 | 400 / 27.5 |
| SW-5001-08-ir | 122 g | 30-60 | 350 / 24.0 |
| SW-5001-120-ir | 180 g | 40-80 | 300 / 20.7 |
| SW-5001-220-ir | 340 g | 50-100 | 300 / 20.7 |
| SW-5001-330-IR | 475 g | 50-100 | 250 / 17.2
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Gome 2. Olentpolash Bandation Ager SEX SHATRIDRIDGEGES. Zipangizo zoloza: Sica womangidwa ndi wosakhazikika wa sax, 40 - 63 μm, 60 Å.
Kuti mumve zambiri pazatsatanetsatane wa seababean ™Makina, kapena chidziwitso cholamula pabwalo la Sturflash Start Cartridges, chonde pitani patsamba lathu.
Post Nthawi: Nov-09-2018