Kugwiritsa kwa C18aq mu Kuyeretsa kwa Polar Peptudel

Rui huang, bo xu
Ntchito R & D Center

Chiyambi
Peptide ndi pawiri yopangidwa ndi amino acid, iliyonse yomwe ili ndi mitundu yapadera yathupi ndi mankhwala chifukwa cha mitundu yosiyanasiyana ndi dongosolo la amino ad imakhazikikanso. Ndi chitukuko cha gawo lolimba la mankhwala synthesis, kapangidwe ka mankhwala kwa m'matumbo osiyanasiyana ogwira kwapita patsogolo kwambiri. Komabe, chifukwa cha kuchuluka kwa peptide komwe kumapezeka ndi kaphatikizidwe kolimba, chinthu chomaliza chimayenera kuyeretsedwa ndi njira zolekanitsa zolekanitsa. Njira zoyeretsa zomwe zimagwiritsidwa ntchito kwambiri pamayendedwe amaphatikizira a ion kusinthidwa kwa chromatography (RP-HPLC) Yofalitsidwa ndi matekinoloje a Santai RP C18 Cartridge idagwiritsidwa ntchito poyeretsa thymopntin (TP-5) ndi zomwe mukufuna kukwaniritsa zofunika.

Chithunzi 1. 20 Amacino wamba acids (kuchokera ku www.bachem.com).

Pali mitundu 20 ya amino acid omwe ali ponseponse pakupanga ma peptides. Andino acid amatha kugawidwa m'magulu otsatirawa malinga ndi malo awo a acid ndi acid: osakhala polar (hydrophobic), polar (monga akuwonetsera). Mu gawo la peptide, ngati amino acid omwe amayambitsa nthawi zambiri amakhala polar (monga tambala wapinki, tysteine, petcheni, enc.ble, ikhale yosungunuka m'madzi. Panthawi ya kuyeretsa kwa polar polant uwu pobwezeretsa ma chromatography, chotchedwa hydrophobic Phase chilengedwe cha Santai: Poyerekeza ndi mzati wa C18 wa C18, mzati wa C18AQ umakhala woyenera kwambiri pakuyeretsa polar kapena ma hydrophilic zitsanzo. Mu positi iyi, peptide wamphamvu ya polar idagwiritsidwa ntchito ngati zitsanzo ndikuyeretsedwa ndi mzati wa C18aq. Zotsatira zake, chinthu chomwe chandamale chimakumana ndi zofunikirazo zidapezeka ndipo zitha kugwiritsidwa ntchito pofufuza zotsatirazi ndi chitukuko.