Hongcheng Wang, Bo xu
Ntchito R & D Center
Chiyambi
Malinga ndi zolemba za curries of the Station gawo ndi gawo lam'manja, ma chromatography amatha kugawidwa kukhala gawo labwino la chromatography (NPC) ndikusintha gawo la chromatography. Kwa RPC, porlaty wa gawo lam'manja ndi lamphamvu kuposa gawo la station. RPC yakhala yogwiritsidwa ntchito kwambiri mu madzi olekanitsidwa amadzimadzi chifukwa cha kuchuluka kwake, kusinthasintha kokwanira komanso njira yotsimikizika. Chifukwa chake RPC ndi yoyenera kulekanitsa ndi kutsuka kwa polar osiyanasiyana kapena polar, kuphatikiza ma acids, manyowa, ma steroa, c18, Phenyl, Conano, amino, ndi zina mwa magulu a magulu awa, omwe amagwiritsidwa ntchito kwambiri C18. Akuyerekeza kuti zoposa 80% ya RPC tsopano ikugwiritsa ntchito C18. Chifukwa chake C18 chrometography colunforn yakhala yofunika kukhala ndi chilengedwe chonse pa labotale iliyonse.
Ngakhale columume c18 itha kugwiritsidwa ntchito pamapulogalamu osiyanasiyana, komabe, pazitsanzo zina zomwe zili polar yomwe ili polar kwambiri kapena ydrophilic kwambiri, mizati yanthawi zonse ya hydrophilic imatha kukhala ndi mavuto poyeretsa zitsanzo izi. Ku RPC, zomwe zimagwiritsidwa ntchito kwambiri zimatha kulamulidwa malinga ndi polarity: madzi <methanol <acetonitrile <ethanol <Tetrapanol. Kuti mutsimikizire kuti musungunuke bwino pa mzere wa zitsanzo izi (polar wamphamvu kapena hydrophilic kwambiri), kuchuluka kwakukulu kwa dongosolo lamadzi ndikofunikira kugwiritsidwa ntchito ngati gawo la mafoni. Komabe, mukamagwiritsa ntchito madzi oyera (kuphatikiza madzi oyera kapena mchere wangwiro) ngati gawo la tebuloni, kaboni wautali pa c18 ndi kusakaniza wina ndi mnzake, zomwe zimapangitsa kuti muchepetse wina aliyense kapena osasungidwa. Izi zimatchedwa "Hydrophobic Gawo la Shase" (monga likuwonekera kumanzere kwa Chithunzi 1). Ngakhale izi zikuwonekeratu pamene mzatiyo amatsukidwa ndi ma soric sol on methanol kapena acetonitrile, iyo imatha kuyambitsa kuwonongeka kwa mzati. Chifukwa chake, ndikofunikira kuti izi zisachitike.
Chithunzi 1. Chithunzi chojambulidwa cha magawo ogwiritsiridwapo pa silika gel mu c18 mzere (kumanzere) ndi c18aq (kumanja).
Kuti tithene ndi mavuto omwe tawatchulawa, zida zonyamula katundu za chromaphic zapanga bwino. Chimodzi mwa zinthuzi chikupanga zosinthika pamtunda wa silika matrix, monga kukhazikitsa ma hydrophilic a cyano (monga akuwonetsera mu gawo loyenera la Chithunzi 1), kupanga mawonekedwe a silika gel hydrophilic. Chifukwa chake maunyolo a C18 pa silika pamtunda amatha kufalikira mokwanira pansi pa mikhalidwe yambiri komanso gawo la hydrophobic lingapewe. Ming'alu yosinthidwa ya c18 imatchedwa mizere yam'madzi yam'madzi yam'madzi, omwe ndi a C18aq, omwe amapangidwira kuti azikhala ndi matenda osokoneza bongo komanso amathanso kulekerera miyeso ya 100%. Mitundu ya C18AQ yaikidwa kwambiri pakulekanitsidwa ndi kuyeretsa kwa ma polar amphamvu, kuphatikizapo organic acid, ma peptic, mavitamini osungunuka ndi mavitamini osungunuka.
Kupanga ndi imodzi mwazinthu zomwe zimagwiritsidwa ntchito ku C18aq mu kuyeretsa kwa zitsanzo, zomwe zimachotsa mchere kapena buffer zikuluzikulu mu zitsanzo zowonjezera kuti zizigwiritsa ntchito kafukufukuyu. Mu positi iyi, fcf ya Blue ndi polarity yolimba idagwiritsidwa ntchito ngati zitsanzo ndikuyeretsedwa pagawo la C18aq. Chitsanzo chosungunulira chinasinthidwa ndi zosungunulira zachilengedwe kuchokera ku yankho la buffer, motero anatsogolera zotsatirazi zosinthika komanso kupulumutsa ma softs ndi nthawi yogwira ntchito. Kuphatikiza apo, chiyero cha Sampuli chidakonzekereratu kuchotsa zodetsa zina mu zitsanzo.
Gawo Loyesera
Chithunzi 2. Kapangidwe ka mankhwala.
Fralsius Blue FCF idagwiritsidwa ntchito ngati zitsanzo zomwe zili munkhaniyi. Kuyera kwa zitsanzo zaiwisi kunali 86% ndipo kapangidwe kake kake kamene kamasonyezedwa. Kukonzekera njira yothetsera blal fcf ya Blue Fral Stualler ndi shook bwino kuti mukhale yankho labwino. Njira yothetsera njirayi idalowetsedwa mu mzere wowonda ndi jakisoni. Kukhazikitsa kwa kuyeretsa kwa Flash kumalembedwa pagome 1.
| Chipangizo | Makina a Sepiban ™2 | |||
| Makatoni | 12 g cuntfalash c18 rp flash cartridge (silika wowoneka bwino, 20 - 45 μm, nambala ya 100 - stå, 522-012 -012 | 12 g cuntfalash c18aq rp flash cartridge (silika wowoneka bwino, 20 - 45 μm, nambala ya orld: SW - 222-012-SP (AQ))) | ||
| Pukhuta | 254 nm | |||
| Gawo lam'manja | Zosungunulira a: madzi Solvent b: Methanol | |||
| Kutentha | 30 ml / min | |||
| Zitsanzo Zotsegulira | 300 mg (Blue Blue Blue ndi Choyera cha 86%) | |||
| Nyumba | Nthawi (CV) | Solvent b (%) | Nthawi (CV) | Solvent b (%) |
| 0 | 10 | 0 | 0 | |
| 10 | 10 | 10 | 0 | |
| 10.1 | 100 | 10.1 | 100 | |
| 17.5 | 100 | 17.5 | 100 | |
| 17.6 | 10 | 17.6 | 0 | |
| 22.6 | 10 | 22.6 | 0 | |
Zotsatira ndi zokambirana
Opatulidwa a C18aq RP Flash Cartridge idagwiritsidwa ntchito pokonza ndi kuyeretsa. Gawo labwino lidagwiritsidwa ntchito pomwe madzi oyera adagwiritsidwa ntchito ngati gawo lam'manja kumapeto kwa eleation ndikuyendetsa ma voliyumu 10 (CV). Monga taonera Chithunzi 3, mukagwiritsa ntchito madzi oyera ngati gawo lam'manja, sampuliyo idasungidwa kwathunthu pa cartridge. Kenako, methanol mu gawo lam'manja lidakwera mwachindunji mpaka 100% ndipo gradient idasungidwa pa 7.5 cv. Sampuli idadetsedwa kuyambira 11.5 mpaka 13.5 cv. M'magawo osonkhanitsa, zitsanzo zosinthazi zidasinthidwa kuchokera ku nah2po4 buffer yankho la methanol. Kuyerekezera ndi njira yayikulu kwambiri, methal inali yosavuta yochotsedwa ndi vatary vataphs mu gawo lotsatira, lomwe limathandizira kafukufuku wotsatirawu.
Chithunzi 3.
Kuti afananitse mayendedwe a C18aq Cartridge ndi C18 Cartridge ya zitsanzo za polarity wamphamvu, mayeso akufananira. Opatulidwa a C18 RP Flash Cartridge idagwiritsidwa ntchito ndi ma ch10 owotcha omwe amawonetsedwa pa Chithunzi 4. Pa ma cartridge a C18, kuchuluka kwambiri kwa gawo loletsedwa ndi 90%. Chifukwa chake choyambirira chidakhazikitsidwa pa 10% methanol mu 90% madzi. Monga taonera Chithunzi 4, chifukwa cha ma hydrophobic Gawo la C18 zomwe zimayambitsidwa ndi chiwerengero chambiri, samputala sichinasungidwe pa C18 Catridge ndipo idasungidwa mwachindunji ndi gawo lam'manja. Zotsatira zake, kugwira ntchito kwa zitsanzo kapena kutsuka sikungamalizidwe.
Chithunzi 4. Flack Chromatogram ya zitsanzo pa cartridge yokhazikika c18.
Kuyerekezera ndi mzere wa mzere, kugwiritsa ntchito sitepe ndi njira zotsatirazi:
1. Kugwiritsa ntchito kugwiritsidwa ntchito ndikuyendetsa nthawi yoyeretsa zitsanzo kumachepetsedwa.
2.
3. Chomwe chosonkhanitsidwa chili ku Methanol chomwe ndi chosavuta kuphedwa, nthawi yopukutira nthawi youma imachepetsedwa.
Pomaliza, chifukwa chotsukidwa kwa zitsanzo zomwe zili mwamphamvu kapena ydrophilic c18aq rp flatridge playsgegraphys sypiden ™.
Za opondera ogwirizanitsa a C18 RP Flash Carridges
Pali mitundu ingapo ya kupatuka kwa c18aq rp flash carridges okhala ndi zojambula zosiyanasiyana kuchokera ku Santai Technology (monga momwe tawonera 2).
| Nambala ya chinthu | Kukula kwa mzere | Kutentha (ml / min) | Max.Press (PSI / Bar) |
| SW-5222-004-SP (AQ) | 5.4 g | 5-15 | 400 / 27.5 |
| SW-522-012-SP (AQ) | 20 g | 10-25 | 400 / 27.5 |
| SW-5222-025-SP (AQ) | 33 g | 10-25 | 400 / 27.5 |
| SW-5222-040-SP (AQ) | 48 g | 15-30 | 400 / 27.5 |
| SW-5222-08-SP (AQ) | 105 g | 25-50 | 350 / 24.0 |
| SW-5222-120-sp (AQ) | 155 g | 30-60 | 300 / 20.7 |
| SW-5222-220-SP (AQ) | 300 g | 40-80 | 300 / 20.7 |
| SW-5222-330-SP (AQ) | 420 g | 40-80 | 250 / 17.2 |
Gome 2. Clealflash C18aq rp flash matorridges.
Zipangizo Zolongedza: Zochita-zokwera kwambiri c18 (AQ) -Kodi silica, 20 - 45 μm, 100 μm, 100 Å.
Logy (monga taonera pa tebulo 2).
Post Nthawi: Aug-27-2018