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Momwe mungalumikizire mizati yopanda kanthu ya iLOK pa Biotage system?
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Kodi silica yogwira ntchito imasungunuka m'madzi?
Ayi, silika wotsekera kumapeto sasungunuka mu zosungunulira zomwe zimagwiritsidwa ntchito nthawi zambiri.
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Kodi ndi mfundo ziti zomwe muyenera kuziganizira mukamagwiritsa ntchito mizati ya C18?
Kuti muyeretsedwe bwino ndi mizati ya C18, chonde tsatirani izi:
① Yatsani gawoli ndi 100% ya zosungunulira zamphamvu (zachilengedwe) za 10 - 20 CVs (voliyumu), nthawi zambiri methanol kapena acetonitrile.
② Yambani ndime ndi 50% yamphamvu + 50% yamadzimadzi (ngati zowonjezera zikufunika, ziphatikizeni) kwa 3 - 5 CVs.
③ Yatsani gawolo ndi mikhalidwe yoyambira ya 3 - 5 CVs. -
Kodi cholumikizira cha mizati yayikulu ndi chiyani?
Pakukula kwa mzati pakati pa 4g ndi 330g, cholumikizira chokhazikika cha Luer chimagwiritsidwa ntchito pazanjazi. Pa kukula kwa 800g, 1600g ndi 3000g, ma adapter owonjezera akuyenera kugwiritsidwa ntchito kuyika mizati ikuluikulu iyi pa chromatography system. Chonde onani chikalata cha Santai Adapter Kit cha 800g, 1600g, 3kg Flash Columns kuti mumve zambiri.
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Kaya cartridge ya silika imatha kuchotsedwa ndi methanol kapena ayi?
Kwa gawo labwinobwino, tikulimbikitsidwa kugwiritsa ntchito gawo la mafoni pomwe chiŵerengero cha methanol sichidutsa 25%.
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Kodi malire ogwiritsira ntchito zosungunulira polar monga DMSO, DMF ndi otani?
Nthawi zambiri, tikulimbikitsidwa kugwiritsa ntchito gawo la mafoni pomwe chiŵerengero cha zosungunulira za polar sichidutsa 5%. Zosungunulira za polar zikuphatikizapo DMSO, DMF, THF, TEA etc.
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Njira zothetsera kutsitsa zitsanzo zolimba?
Kuyika kwachitsanzo cholimba ndi njira yothandiza pakuyika zitsanzo kuti ziyeretsedwe pazanja, makamaka zitsanzo za kusungunuka kochepa. Pankhaniyi, iLOK kung'anima katiriji ndi yabwino kwambiri.
Nthawi zambiri, chitsanzocho chimasungunuka mu chosungunulira choyenera ndikumangidwira pa adsorbant yolimba yomwe ingakhale yofanana ndi yomwe imagwiritsidwa ntchito muzitsulo zonyezimira, kuphatikizapo diatomaceous earths kapena silika kapena zipangizo zina. Pambuyo pochotsa / kutuluka kwa zosungunulira zotsalira, adsorbent imayikidwa pamwamba pa ndime yodzaza pang'ono kapena mu cartridge yopanda kanthu. Kuti mumve zambiri, chonde onani chikalata cha iLOK-SL Cartridge User Guide kuti mumve zambiri. -
Kodi njira yoyesera ya voliyumu yamtundu wa flash ndi iti?
Voliyumu yazanja ndi pafupifupi yofanana ndi voliyumu yakufa (VM) ponyalanyaza voliyumu yowonjezera mu machubu olumikiza ndime ndi jekeseni ndi chowunikira.
Nthawi yakufa (tM) ndi nthawi yofunikira kuti chinthu chomwe sichinasungidwe chiwoneke.
Voliyumu yakufa (VM) ndiye kuchuluka kwa gawo la foni lomwe limafunikira pakuwunikira chinthu chosasungidwa. Voliyumu yakufa imatha kuwerengedwa ndi equation yotsatirayi:VM =F0*tM.
Pakati pa equation yomwe ili pamwambapa, F0 ndiye kuchuluka kwa gawo la mafoni.
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Kodi silica yogwira ntchito imasungunuka mu methanol kapena zina mwazinthu zina zosungunulira organic?
Ayi, silika wotsekera kumapeto sasungunuka mu zosungunulira zomwe zimagwiritsidwa ntchito nthawi zambiri.
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Kaya silika kung'anima katiriji angagwiritsidwe ntchito mobwerezabwereza kapena ayi?
Mizati ya silika imatha kutaya ndipo imagwiritsidwa ntchito kamodzi, koma pogwira bwino, makatiriji a silica amatha kugwiritsidwanso ntchito popanda kudzipereka.
Kuti agwiritsidwenso ntchito, gawo la silika liyenera kuwumitsidwa ndi mpweya woponderezedwa kapena kutenthedwa ndikusungidwa mu isopropanol. -
Kodi mikhalidwe yoyenera kutetezedwa kwa C18 flash cartridge ndi iti?
Kusungirako koyenera kudzalola kuti mizere ya C18 igwiritsidwenso ntchito:
• Musalole kuti mzatiwo uume mukatha kugwiritsa ntchito.
• Chotsani zosintha zonse za organic potsitsa gawoli ndi 80% methanol kapena acetonitrile m'madzi kwa 3 - 5 CVs.
• Sungani mzati mu zosungunulira zomwe tazitchulazi zomwe zili m'malo mwake. -
Mafunso okhudza momwe kutentha kumagwirira ntchito mu pre-equilibrity process for flash columns?
Pazipilala zazikuluzikulu zomwe zili pamwamba pa 220g, kutentha kumawonekera pokonzekera kulinganiza. Ndibwino kuti muyike mlingo wothamanga pa 50-60% ya mlingo womwe ukuperekedwa mu ndondomeko ya pre-equilibrium kuti mupewe zotsatira zoonekeratu za kutentha.
The matenthedwe zotsatira za zosungunulira wosanganiza n'zoonekeratu kuposa zosungunulira limodzi. Tengani zosungunulira za cyclohexane/ethyl acetate monga mwachitsanzo, akulimbikitsidwa kuti agwiritse ntchito 100% cyclohexane mu ndondomeko ya pre-equilibrium. Kulinganiza kusanachitike kumalizidwa, kuyesa kulekanitsa kungathe kuchitidwa molingana ndi dongosolo losungunulira lokhazikitsidwa kale.
