Rui Huang, Bo Xu
Taupānga R&D Center
Kupu Whakataki
Ko te chromatography whakawhiti katote (IEC) he tikanga chromatographic e whakamahia nuitia ana ki te wehe me te pure i nga pūhui e whakaatuhia ana i roto i te ahua katote i roto i te otinga.E ai ki nga ahuatanga utu rereke o nga katote whakawhiti, ka taea te wehea te IEC ki nga momo e rua, te chromatography whakawhiti cation me te chromatography whakawhiti anion.I roto i te chromatography whakawhiti cation, ka herea nga roopu waikawa ki te mata o te papaho wehewehe.Hei tauira, ko te waikawa sulfonic (-SO3H) he roopu e whakamahia nuitia ana i roto i te whakawhitinga ngongo kaha (SCX), e wehewehe ana i te H+ me te roopu toraro -SO3- ka taea te whakauru i etahi atu katote i roto i te otinga.I roto i te chromatography whakawhiti anion, ka herea nga roopu kawakore ki te mata o te papaho wehewehe.Hei tauira, ko te amine quaternary (-NR3OH, kei reira te R ko te roopu waro waro) ka whakamahia i roto i te whakawhiti anion kaha (SAX), e wehewehe ana i te OH- me te roopu pai -N+R3 ka taea te whakauru i etahi atu anion i roto i te otinga, ka puta he anion. pānga whakawhiti.
I roto i nga hua taiao, ko nga flavonoids kua aro ki nga kairangahau na te mea ko o raatau mahi ki te aukati me te maimoatanga o nga mate ngakau.I te mea he waikawa nga ngota ngota flavonoid na te noho mai o nga roopu hydroxyl phenolic, ko te chromatography whakawhiti katote he whiringa kee hei taapiri atu ki te waahanga noa, ki te waahanga hurihuri ranei mo te wehenga me te purenga o enei pūhui waikawa.I roto i te chromatography uira, ko te whakamaarama wehe katoo mo te whakawhiti katote ko te matrix gel silica ka herea nga roopu whakawhiti katote ki tona mata.Ko nga tikanga whakawhiti katote e whakamahia nuitia ana i roto i te chromatography flash ko te SCX (te nuinga o te roopu waikawa sulfonic) me te SAX (te nuinga o te roopu amine quaternary).I roto i te tuhipoka tono i whakaputaina i mua me te taitara "Te Whakamahinga o SepaFlash Strong Cation Exchange Chromatography Columns in the Purification of Alkaline Compounds" na Santai Technologies, i whakamahia nga pou SCX mo te purenga o nga puhui kawakore.I tenei pou, he ranunga o nga paerewa kore me te waikawa i whakamahia hei tauira ki te torotoro i te whakamahinga o nga pou SAX i roto i te purenga o nga pūhui waikawa.
Wāhanga Whakamātau
Whakaatu 1. Ko te hoahoa hoahoa o te wahanga noho e herea ana ki te mata o te papaaho wehewehe SAX.
I tenei whakairinga, i whakamahia he pou SAX kua whakakiia ki te silica herea amine quaternary (penei i te Whakaaturanga 1).Ko te ranunga o te Chromone me te waikawa 2,4-dihydroxybenzoic i whakamahia hei tauira hei pure (penei i te Whakaaturanga 2).I rewa te ranunga ki te methanol, ka utaina ki runga i te kaata uira e te injector.Ko te tatūnga whakamātautau o te purenga uira kua whakarārangihia ki te Ripanga 1.
Whakaatu 2. Te hanganga matū o nga wahanga e rua o te ranunga tauira.
| Taputapu | Miihini SepaBean™ T | |||||
| Kaata | 4 g SepaFlash Standard Series kariri kohiko (silica kōhikohiko, 40 - 63 μm, 60 Å, Tau ota: S-5101-0004) | 4 g SepaFlash Bonded Series SAX kariri kohiko (silica kōhikohiko, 40 - 63 μm, 60 Å, Tau ota:SW-5001-004-IR) | ||||
| Te roangaru | 254 nm (kitenga), 280 nm (aroturuki) | |||||
| Waahanga pūkoro | Wairewa A: N-hexane | |||||
| Wairewa B: Ethyl acetate | ||||||
| Rere rere | 30 mL/min | 20 mL/min | ||||
| Uta tauira | 20 mg (he ranunga o te Wae A me te Wae B) | |||||
| Rōnaki | Wā (CV) | Wairewa B (%) | Wā (CV) | Wairewa B (%) | ||
| 0 | 0 | 0 | 0 | |||
| 1.7 | 12 | 14 | 100 | |||
| 3.7 | 12 | / | / | |||
| 16 | 100 | / | / | |||
| 18 | 100 | / | / | |||
Nga Hua me te Matapaki
Tuatahi, ka wehea te ranunga tauira e te kariri kohiko wahanga noa kua whakakiia ki te silica auau.E ai ki te Whakaaturanga 3, ko nga wahanga e rua i roto i te tauira i whakahekehia mai i te kariri tetahi i muri i tetahi.I muri mai, ka whakamahia he kariri rama SAX mo te purenga o te tauira.E whakaatu ana i te Whakaahua 4, i mau tonu te Wae B waikawa ki te kariri SAX.Ko te Waahanga Aa-a-waa i whakahekehia mai i te kariri me te whakamaaramatanga o te wahanga pūkoro.
Whakaahua 3. Ko te chromatogram rama o te tauira i runga i te kariri wahanga noa.
Whakaatu 4. Te chromatogram rama o te tauira i runga i te kariri SAX.
Ki te whakatairite i te Whakaahua 3 me te Whakaahua 4, he rereke te ahua o te Waa A ki runga i nga kariri kohiko rereke e rua.Hei whakaū mena kei te rite te tihi o te elution ki te waahanga, ka taea e taatau te whakamahi i te waahanga matawai roangaru katoa i hangaia ki roto i te punaha whakahaere o te miihini SepaBean™.Whakatuwherahia nga raraunga whakamatautau o nga wehenga e rua, toia ki te raina tohu i runga i te tuaka wa (CV) i roto i te chromatogram ki te pito teitei me te pito tuarua teitei o te tihi elution e rite ana ki te Waahanga A, me te whanuitanga roangaru o enei e rua. ka whakaatu aunoa nga tohu i raro i te chromatogram (penei i te Whakaahua 5 me te Whakaahua 6).Ko te whakatairite i nga raraunga hihinga roangaru katoa o enei wehenga e rua, he rite tonu te awhi whakaurunga o te Wae A i roto i nga whakamatautau e rua.Na te mea he rereke te ahua o te Waa A i runga i nga kaata rama rereke e rua, e whakapaetia ana he poke motuhake kei roto i te Waa A he rereke te pupuri i te kariri wahanga noa me te kariri SAX.Na reira, he rereke te raupapa eluting mo te Wae A me te poke o enei kaata kohiko e rua, na reira ka kore e rite te ahua o te tihi ki runga i nga chromatograms.
Whakaatu 5. Ko te roangaru roangaru katoa o te Wae A me te poke i wehea e te kariri wahanga noa.
Whakaatu 6. Ko te roangaru ngaru katoa o te Wae A me te poke i wehea e te kariri SAX.
Mēnā ko te hua ka kohia ko te Waahanga A kūpapa, ka ngawari te whakaoti i te mahi purenga ma te whakamahi tika i te kariri SAX mo te whakamaarama i muri i te utaina tauira.I tetahi atu taha, mena ko te hua e kohia ana ko te Wae B waikawa, ka taea te whakamahi i te tikanga hopu-tuku me te paku whakarereketanga i nga waahanga whakamatautau: i te wa i utaina ai te tauira ki te kariri SAX me te Wae A koretake. i whakakorea katoatia me nga whakarewa pararopi noa, huri i te wahanga pūkoro ki te wairewa methanol kei roto 5% waikawa ahetiki.Ka whakataetae nga katote acetate i roto i te wahanga pūkoro ki te Wae B mo te here ki nga roopu katote amine quaternary i runga i te wahanga tu o te kariri SAX, na reira ka peia te Wae B mai i te kaata kia whiwhi ai i te hua kua whakaritea.Ko te chromatogram o te tauira kua wehea i roto i te aratau whakawhiti katote i whakaatuhia i te Whakaahua 7.
Whakaahua 7. Ko te chromatogram uira o te Waa B i whakahekehia i roto i te aratau whakawhiti katote i runga i te kariri SAX.
Hei whakamutunga, ka tere te purea te tauira waikawa, koretake ranei e te kariri SAX me te kariri wahanga noa e whakamahi ana i nga rautaki purenga rereke.I tua atu, ma te awhina o te waahanga matawai roangaru katoa i hangaia ki roto i te punaha whakahaere o te miihini SepaBean ™, ka taea te whakataurite me te whakaū i te awheawhe whakaurunga o nga hautau kua whakahekehia, e awhina ana i nga kairangahau ki te whakatau tere i te hanganga me te ma o nga hautau kua whakahekehia, na reira ka pai ake. mahi pai.
| Tau Tuemi | Rahi Tīwae | Rere Rere (mL/mene) | Max.Pēhanga (psi/pa) |
| SW-5001-004-IR | 5.9 g | 10-20 | 400/27.5 |
| SW-5001-012-IR | 23 g | 15-30 | 400/27.5 |
| SW-5001-025-IR | 38 g | 15-30 | 400/27.5 |
| SW-5001-040-IR | 55 g | 20-40 | 400/27.5 |
| SW-5001-080-IR | 122 g | 30-60 | 350/24.0 |
| SW-5001-120-IR | 180 g | 40-80 | 300/20.7 |
| SW-5001-220-IR | 340 g | 50-100 | 300/20.7 |
| SW-5001-330-IR | 475 g | 50-100 | 250/17.2
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Ripanga 2. SepaFlash Bonded Series SAX flash cartridges.Ko nga taonga tapaki: Ultra-parakore kohikohiko te kirika herea SAX, 40 - 63 μm, 60 Å.
Mo etahi atu korero mo nga korero taipitopito o SepaBean™miihini, me nga korero ota mo nga kaata rama raupapa SepaFlash, tirohia to maatau paetukutuku.
Te wa tuku: Nov-09-2018