Meiyuan Qian, Yuefeng Tan, Bo Xu
Applicationem R & D Center
Introductio
Taxus (Taxus chinensis vel Seres) est feram planta tuta in regionem suam. Est rara et periclitari planta reliquit a tergo a quaternarium molibus. Est etiam solum naturalis medicinalis in mundo. Taxus distribuitur in temperante zonam de septemtrionis hemisphaerio usque ad medium subtropical regionem, cum circa XI speciei in hoc mundo. Sunt IV speciei et I varietate in Sina, nimirum Northeast Taxus, Yunnan Taxus Taxus, Tibetan Taxus et Southern Taxus. Hi quinque species sunt distribuit in Sinis Sina, Sinis Sina, Central Sina, Sina Sina, Sina Service Service Service et Taiwan. Taxus plantis continent a varietate chemical components, comprehendo taxanes, flavonoids, Lignans, steroids, phenolic acida, sesquires et glycosides. Et celebre anti-tumor medicamento taxol (vel Paclitaxel) est quaedam taxanes. Taxol habet unique anticancer machinationes. Taxol potest "Frigidus" Microtubules per pectentes cum illis et ne microtubules a separando chromosomatum ad tempus cellulis, ita ducit ad mortem dividendo cellulis, praesertim cursim celeriter cancer cellulis [I]. Ceterum activating macrophages, taxol causat decrementum in TNF-α (tumore necrosis factor) receptores et dimittere TNF-α, ita occidere vel inhibentes tumorem cellulis [II]. Porro Taxol potest inducere apoptosis per agens in Apoptotic receptor via mediantur a FAS / Fasl aut activum Cysteine protease system [III]. Due to its multiple target anticancer effect, Taxol is widely used in the treatment of ovarian cancer, breast cancer, non-small cell lung cancer (NSCLC), gastric cancer, esophageal cancer, bladder cancer, prostate cancer, malignant melanoma, head and neck cancer, etc[4]. Praesertim pro Advanced pectus cancer et provectus ovarii cancer, taxol habet praestantes curativa effectus, ergo quod est ut 'ultimum linea defensionis pro cancer curatio. "
Taxol est maxime popular anticancer medicamento in internationalis foro in recens annos et consideretur esse unum ex maxime anticancer medicinae ad homines in altera XX annis. In annis, cum explosivae incrementum de populatio et cancer incidentiae, in demanda pro taxol etiam augetur significantly. Currently, taxol requiritur ad orci vel scientificam investigationis maxime extrahitur directe ex Taxus. Infeliciter, in content of taxol in plantis satis humilis. Exempli gratia, in taxol contentus est solum 0.069% in cortice of Taxus brevifolia, quae plerumque considerari habere summum contentus. Nam extraction of I g de taxol, quod postulat de 13.6 kg de Paxus cortice. Ex hoc estimate, capit III - XII taxus ligna quae sunt plus quam C annorum, ut tractare ovarian cancer patientes estote. Sicut effectus, numerus of taxus arbores sunt interficiam descendit, unde in proximo exstinguendo hoc pretiosa speciebus. In addition, Taxus est valde pauper in opibus et tardus in incrementum, quod facit difficile ad longius progressionem et utendo de taxol.
In praesenti, in summa synthesis Taxol est feliciter perficitur. Tamen, eius synthetica route valde universa et summus sumptus, faciens illud habere non industriae momenti. Et semi-synthetica methodus Taxol nunc relative mature et consideretur esse efficax ad expand fontem Taxol in addition artificialis plantabant. Breviter, in semi-synthesim of taxol, et taxol precursor compositis, quae est relative abundat in taxus plantis extrahitur et convertitur in taxol per eget synthesis. In content of X-deacetyslbaccatin ⅲ in acus Pinus Baccata potest esse usque ad 0.1%. Et acus facile regenerare comparet cum latrat. Ideo in semi-synthesim of taxol secundum X, deacetyslbaccatin ⅲ est attracting magis et magis operam ex investigatores [V] (ut ostensum est in Figura I).
Figura I. De semi-synthetica Taxol secundum X, deacetyslbaccatin ⅲ.
In hoc post, in taxus plant extract purificatum a Flash praeparative Liquid Chromatography Sepabean ™ Apparatus in combination cum Sepaillash C18 Reversed-Phase (RP) Flash Cartridges produci per Santai Technologies. In scopum productum occurrens in puritate requisita est adeptus et potest esse in subsequent scientificis investigationibus, offering sumptus-effective solution pro celeri purificatione huius generis naturalis products.
| Instrumentum | Sepabean ™ Machina | |
| Cartridge | XII G Sepeflashsh C18 RP Flash Cartridge (Sphaerical Silica, XX - 45μm, C a, ut Number: Sw-5222-012, SP) | |
| RECONCINNUS | CCLIV NM (Deprehensio), CCLXXX NM (Cras) | |
| Mobile Phase | Solvent A: Aqua | |
| Solvent B: Methanol | ||
| Rate fluunt | XV ml / min | |
| Sample loading | XX mg crudum sample dissolvi in I ml dmso | |
| Clivus | Tempus (min) | Solvent B (%) |
| 0 | 10 | |
| 5 | 10 | |
| 7 | 28 | |
| 14 | 28 | |
| 16 | 40 | |
| 20 | 60 | |
| 27 | 60 | |
| 30 | 72 | |
| 40 | 72 | |
| 43 | C | |
| 45 | C | |
Results et disputationem
Et Flash chromatogram pro cruda eliciunt ex taxus ostensum est in Figura II. Per analyzing chromatogram, in scopum productum et impudicitiis effectum baseline separationem. Praeterea, bonum reproducibility etiam cognovit per plures specimen injections (notitia non ostensum est). Is mos sumo de IV horas ad perficere separationem in manual chromatography modum in speculo columnas. Comparet cum traditional manual chromatography modum, in automatic purificationem modum in hoc post solum requirit XLIV minuta ad perficere totum purificationem negotium (ut ostensum est in Figura III). Plus quam LXXX% of tempus et magnum moles solvendo potest salvus per ipsum modum, quae potest efficaciter reducere sumptus tum magna amplio opus efficientiam.
Figura II. Et Flash chromatogram de cruda ex extract ex Taxus.
Figura III. De comparatione manual chromatography modum in ipso purificatione modus.
In conclusione, Pectore Sepaflash C18 RP Flash Cartridges cum Sepabean ™ apparatus potest offerre ieiunium et efficient solutio ad celeri purificationem naturalis products ut taxus extractum.
References
1. Alushin GM, Lander GC, Kellogg EH, Zhang R, Baker D and Nogales E. High-resolution microtubule structures reveal the structural transitions in αβ-tubulin upon GTP hydrolysis. Cell, MMXIV, CLVII (V), 1117-1129.
II. Burkhart CA, Berman JW, Swindell CS et Horwitz si. Necessitudinem inter structuram de taxol et alia taxanes in inductione tumore necrosis factor-α gene expressio et cytotoxicity. Cancri Research, MCMXCIV, LIV (XXII), 5779-5782.
III. Park SJ, Wu C, Gordon JD, Zhong X, Emami A et Safa AR. Taxol inducit Caspase-X, dependens apoptosis, J. Biol. Cham., MMIV, CCLXXIX, 51057-51067.
IV. Paclitaxel. Americae Society of Salutem, Ratio Pharmacists. [Ianuarii II, MMXV]
V. Brus Ganem Roland R. Franke. Paclitaxel de prima taxanes: A perspective in partum invention in organozirconium elit. J. Org. Cham., MMVII, LXXII (XI), 3981-3987.
Sunt a series in Sepaillash C18 RP Flash Cartridges cum diversis specifications a Santai technology (ut ostensum est in mensa II).
| Item numerus | Columna | Rate fluunt (M / min) | Max.pressure (Psi / Bar) |
| Sw-5222-004, sp | 5.4 g | 5-15 | CD / 27.5 |
| Sw-5222-012, sp | XX g | 10-25 | CD / 27.5 |
| Sw-5222-025-sp | XXXIII g | 10-25 | CD / 27.5 |
| Sw-5222-040-sp | XLVIII G | 15-30 | CD / 27.5 |
| Sw-5222-080-sp | CV g | 25-50 | CCCL / 24.0 |
| Sw-5222-120-sp | CLV g | 30-60 | CCC / 20.7 |
| Sw-5222-220-sp | CCC g | 40-80 | CCC / 20.7 |
| Sw-5222-330-sp | CDXX G | 40-80 | CCL / 17.2 |
Table II. Sepaflash C18 RP Flash Cartridges.
Stipare materiae, summus efficientiam sphaerici C18-Bonded Silica, XX - XLV μ, C a
Nam ulteriores notitia in detailed specifications Sepabean ™ apparatus, aut ordinatio notitia in Series Series Cartridges, placere visitare noster website
Post tempus: Sep, 20-2018