Mingzu Yang, Bo Xu
Applicationem R & D Center
Introductio
Antibiotics sunt classis secundarium metabolitarum produci per microorganisms (including bacteria, fungi, Actinomycetes) componit, quae sunt chemica synthesized vel semi-synthesized. Antibiotics posset inhibere incrementum et salvos de aliis microorganisms. In prima antibiotic inventa per humanum, penicillin, inventa per Britanniam Microbiologist Alexander Fleming in MCMXXVIII. Notavit quod Bacteria in vicinia fingunt non crescere in Staphylococcus Cultum quod contaminari potuit fingunt. Et postulatum est fingunt debet secretum an antibacterial substantia, quae nominatur penicillin in MCMXXVIII. Sed activa ingredientia non purificati in illo tempore. In MCMXXXIX, Ernst torquem et Howard florey of Oxford University placuit ut develop a medicamento quod posset tractare bacterial infectiones. Post contingentes Fleming obtinere fervat feliciter extraxerunt et purificatum Penicillin a modica. Nam eorum felix progressionem Penicillin sicut therapeutic medicamento, Fleming, torquem et Florey participatur in MCMXLV Nobel praemium in medicina.
Antibiotics sunt ut antibacterial agentibus tractare vel ne bacterial Infectiones. Sunt pluribus principalis genera antibiotics usus est antibacterial agentibus, β-Lactam antibiotics (including penicillin, cephalosporin, etc.), aminoglycoside antibiotics, Macrolide antibiotics, tetratic includit biological fermentation (totalis antibiotics includit biological fermentation (Total antibiotics includit biological fermentationem, semi-synthesis et summa synthesis. In antibiotics produci per biologicum fermentationem postulo ut structuram modi modi ex chemical stabilitatem, toxicus latus effectus, antibacterial spectro et aliis exitibus. Post chemically mutatio, antibiotics non consequi auctus stabilitatem, reducitur toxicus latus effectus, expanded antibacterial spectro, reducitur medicamento resistentia, melius bioavailability, et ita melius effectus medicamento curatio. Ideo semi-synthetica antibiotics sunt currently maxime popularis directionem in progressionem antibiotic pharmaca.
In progressionem in semi-synthetica antibiotics, antibiotics habent proprietatibus humilis castitatis, sortem ex-products et complexu components quia sunt ex microbial fermentationem products. In hoc casu, in analysis et imperium impudicitiis in semi-synthetica antibiotics est praecipue magna. Ut efficaciter identify et characterize impurities, necesse est obtinendum sufficientem impudicitiis a Saccharum productum semi-synthetica antibiotics. Inter communiter adhibetur immunditia praeparatio techniques, mico chromatography est sumptus-effective modum cum commoda ut magna sample loading quantum, humilis sumptus, tempus salutaris, etc. Flash Homo est magis et magis usus per syntheticam inquisitores est magis et magis usus est in synthetography.
In hoc post, pelagus immunditia a semi-synthetica aminoglycoside antibiotic erat usus est in sample et purificatum per a separabis C18aq cartridge combined cum Flash Chromatography Sepabean ™ apparatus. In scopum productum occurrens requisita prospere adeptus, suggerente a valde efficient solutio ad purificationem harum componit.
Sectionalis
Et sample fuit misericordiam provisum est a locorum pharmaceutical company. Et sample erat quaedam amino polycyclic carbohydrates et eius mulgeular structuram erat similis cum aminoglycoside antibiotics. Polarity de sample erat alta, faciens solutum aqua. Et schematic diagram de sample scriptor mulgetam structuram in figura I. De puritate et rudis sample erat de LXXXVIII% ut resolvitur per Hplc. Nam purificatio horum componit altus verticitatem, sample vix retenta in iusto C18 columnas secundum priorem experientiam. Ideo a C18AQ columna usus est ad sample purificationem.
Figura I. Et schematic diagram de sample scriptor structuram.
Ut parare sample solutio, L mg mg cruda sample est dissolvi in V ml pura aqua et tunc ultrasonicated ut faciam illud facti omnino patet solutio. Et sample solution est tunc infusum in mico columna per injector. In experimentalem setup de mico purificatione fuit enumerantur in mensa I.
| Instrumentum | Sepabean ™ Machina II | |
| Cartridges | XII G separeflash c18aq rp mico cartridge (sphaericam Silica, XX - 45μm, C a, ut Number: Sw-5222-012, SP (AQ)) | |
| RECONCINNUS | CCIV Nm, CCXX NM | |
| Mobile Phase | Solvent A: Aqua Solvent B: Acathetonitrile | |
| Rate fluunt | XV ml / min | |
| Sample loading | L mg | |
| Clivus | Tempus (min) | Solvent B (%) |
| 0 | 0 | |
| 19.0 | 8 | |
| 47.0 | 80 | |
| 52.0 | 80 | |
Results et disputationem
In Flash chromatogram de sample in c18aq cartridge fuit ostensum est in Figura II. Sicut ostensum est in Figura II, in altus Suspendisse sample erat efficaciter retinuit in c18aq cartridge. Post lyopholizacionem ad collected fractiones, in scopum productum habuit puritatem 96.2% (ut ostensum est in Figura III) by HPLC Analysis. Eventus indicavit quod purificatum productum posse esse ulterius assuetudo in proximo gradum investigationis et progressionem.
Figura II. In Flash chromatogram de sample in c18aq cartridge.
Figura III. In HPRCH Chromatogram de target uber.
In conclusione, Sepaflash c18aq RP Flash Cartridge cum Flash Chromatography Sepabean ™ apparatus potuit offerre ieiunium et effective solutio ad purificationem altus polaris exempla.
De Series C18AQ RP Flash Cartridges
Sunt series in Sepaflash c18aq RP mico cartridges cum diversis specifications a Santai technology (ut ostensum est in Table II).
| Item numerus | Columna | Rate fluunt (M / min) | Max.pressure (Psi / Bar) |
| Sw-5222-004-SP (AQ) | 5.4 g | 5-15 | CD / 27.5 |
| Sw-5222-012, SP (AQ) | XX g | 10-25 | CD / 27.5 |
| Sw-5222-25-SP (AQ) | XXXIII g | 10-25 | CD / 27.5 |
| Sw-5222-040-SP (AQ) | XLVIII G | 15-30 | CD / 27.5 |
| Sw-5222-080-SP (AQ) | CV g | 25-50 | CCCL / 24.0 |
| Sw-5222-120, SP (AQ) | CLV g | 30-60 | CCC / 20.7 |
| Sw-5222-220-SP (AQ) | CCC g | 40-80 | CCC / 20.7 |
| Sw-5222-330-SP (AQ) | CDXX G | 40-80 | CCL / 17.2 |
Table II. Sepaflash c18aq RP mico cartridges. Stipare materiae, summus efficientia sphaerici C18 (AQ) -Bonded Silica, XX - μm XLV, C a.
Nam ulteriores notitia in detailed cubits of Sepabean ™ apparatus, aut ordinatio notitia in Series Series Cartridges, placere visitare noster website.
Post tempus: Oct-26-2018