Rui Huang, Bo Xu
Applicationem R & D Center
Introductio
Ion Exchange Chromatography (IEC) est chromatographic modum communiter ad separandum et purificat compositorum, quae sunt in Ionica forma in solution. Secundum diversas causam civitatibus exchangeable ions, IEC potest dividi in duo genera, catione commutationem chromatography et anion commutationem chromatergro. In catione chromatography, acidic coetibus sunt vincula in superficiem separationis media. Exempli gratia, sulfonic acidum (-so3h) est communiter in forti catione commutatione (SCX), quod Disociates H + et negative præcepit Group -So3- potest sic adsorb cessi in solut. In anion commutationem chromatography, alkaline coetibus sunt religata ad superficiem separationis media. Nam exempli gratia, Quaternarium Amine (-nr3Oh, ubi R est Hydrocarbon Group) solet usus est in fortis anion commutationem (Saxo), quae dissociates oh- et positive in solutione, unde in anion commutatione effectus.
Inter naturalis products, flavonoids ad attrahitur operam de investigatores propter eorum munus in praeventionis et curatio de cardiovascular morbis. Quia flavonoid moleculis sunt acidic debitum ad praesentiam of phenolic hydroxyl coetus, Ion Exchange Chromatography est alternative optio praeter institutum normalis tempus vel reversed Phase Croomatergraph ad separationem et purificationem harum acidic componit. In Flash Chromatography, communiter solebat separationem media ad Ion commutationem est Silica Gel Matrix ubi Ion commutationem coetibus sunt in eius superficiem. Maxime communiter usus ion commutationem modi in mico chromatography sunt scx (plerumque sulfonic acidum coetus) et Sax (plerumque quaternarium Amine Group). In ante published Application Nota cum titulo "applicationem de Sepeflash fortis cationem commutationem chromatography columnas in purificationem alkaline componit" per Santai Technologies, Scx columnas sunt usus ad purificationem alkaline componit. In hoc post, mixturam neutrum et acidic signa usus est ut sample explorarent applicationem of Sax columnas in purificatione acidic componit.
Sectionalis
Figura I. Et schematic diagram in stabilem tempus religata ad superficiem Saxboni separationis media.
In hoc post, in Sax columna Pre-facis cum Quadernary Amine Bonded Silica erat usus (ut ostensum est in Figura I). A mixtisque chromone et 2,4-dihydroxybenzoic acidum est usus ut sample ut purificari (ut ostensum est in Figura II). Et mixtisque dissolvi in Methanol et oneratis onto in Flash Cartridge per injector. Et experimentum setup de mico purificatione est enumerantur in mensa I.
Figura II. Et eget structuram de duabus components in specimen mixturam.
| Instrumentum | Sepabean ™ Machina T | |||||
| Cartridges | IV G Sepeflash Latin Edition Series Cartridge (Irregular Silica, XL - LXIII μm, LX Å, ut Number: S-5101-0004) | IV G separacshard Bonded Saxo Sax Flash Cartridge (Irregular Silica, XL - LXIII μm, LX å, ut Number: Sw-5001-004-IR) | ||||
| RECONCINNUS | CCLIV NM (Deprehensio), CCLXXX NM (Cras) | |||||
| Mobile Phase | Solvent A: N-Hexane | |||||
| Solvent B: ethyl acetate | ||||||
| Rate fluunt | XXX ml / min | XX ml / min | ||||
| Sample loading | XX mg (a mixtisque component a et component b) | |||||
| Clivus | Tempus (CV) | Solvent B (%) | Tempus (CV) | Solvent B (%) | ||
| 0 | 0 | 0 | 0 | |||
| 1.7 | 12 | 14 | C | |||
| 3,7 | 12 | / | / | |||
| 16 | C | / | / | |||
| 18 | C | / | / | |||
Results et disputationem
Uno modo, sample mixtisque separata a normalis phase Flash Cartridge pre-facis cum regularis silica. Ut ostenderet in Figura III, duo components in sample erant eluted ex cartridge unum post alterum. Deinde, in Sax Flash Cartridge est usus ad purificationem specimen. Ut ostenderet in Figura IV, in acidic pars B est omnino retinuit in Sax Martridge. Quod neutrum pars paulatim eluted ex cartridge cum eloquium mobile tempus.
Figura III. In Flash chromatogram de sample in ordinarius normalis tempus cartridge.
Figura IV. Et Flash chromatogram de sample in Sax Martridge.
Comparet Figura III et Figura IV, quod component a repugnat apicem figura in duas diversas mico cartridges. Ad confirmandas sive ad eludendum apicem correspondentes ad component, possumus utilitas plenum intus scanning pluma quae aedificatur in imperium software Sepabean ™ apparatus. Open the experimental data of the two separations, drag to indicator line on the time axis (CV) in the chromatogram to the highest point and the second highest point of the elution peak corresponding to the Component A, and the full wavelength spectrum of these two points will be automatically shown below the chromatogram (as shown in Figure 5 and Figure 6). Comparet plenam unam domesticis notitia de his duo separationes, ad component a consistent effusio spectro in duas experimenta. Nam ratio ad componentium a repugnat apicem figura in duas diversas mico cartridges, id est speculatio, quod est specifica immunditia in component a quo habet alium retentionem in normalis tempus cartridge et Sax Martridge. Ergo eluting serie differt ad component et immunditia in his duobus mico cartridges, unde repugnat apicem figura in chromatograms.
Figura V. In plenam domicilium spectro de component a et immunditia separata a normalis tempus cartridge.
Figura VI. Quod plenam domicilium spectro de component a et in inmunditia separata a Sax Martridge.
Si target uber ut colligi est neutrum component a, purificatio opus facile perficitur per directe per Sax Martridge ad Helium cum sample loading. In alia manu, si target uber ut collecta est acidic pars B, captis, release modo potest esse adoptatus cum tantum levi temperatio in experimentalem et neutrum est in Loaded in SAX in Mobile tempus ad Methanol solvente, switch et in Mobile Phase in Methanol solutionum continet ad Mobile Phase et Methanol Suspendisse in Mobile Phase et Methanol Suspendisse in Mobile et Methanol SOLITUDING ACETICUS Methanol in solutionum, cum in Mobile ACETICUS et Methanol SOLITUDING PARHANOL CARNHANOL CARRIDENTUM et Mobile ACETICUS ad Methanol SOLITUDING EST in Mobile ACITIC ACIDUM. Et acetate ions in mobile tempus Certatim cum component b ad binding to the Quaesternary Amine Ion coetibus in stationarius tempus de Sax Cartridge, ita eluting ad b a cartridge ad target productum. Et chromatogram de sample separata in Ion commutationem modus est ostensum est in Figura VII.
Figura VII. In Flash chromatogram de component b elutedit in Ion commutationem modus in Sax Martridge.
In conclusione, acidic aut neutrum sample potest esse cursim purificatum per Saxapter cartridge combined cum normalis tempus cartridge utilizing diversis purificatione consiliantur. Ceterum cum auxilio plena intus scanning pluma ædificavit in potestate software of Sepabean ™ apparatus, proprietatem effusio spectro de eluted fractiones posset determinare et confirmavit, auxilium investigatores cito determinare compositionem et inquisitores inquisitores cito determinare compositionem efficientiam elit.
| Item numerus | Columna | Rate fluunt (M / min) | Max.pressure (Psi / Bar) |
| Sw-5001-004-ir | 5.9 g | 10-20 | CD / 27.5 |
| Sw-5001-012, ir | XXIII g | 15-30 | CD / 27.5 |
| Sw-5001-025-ir | XXXVIII G | 15-30 | CD / 27.5 |
| Sw-5001-040-ir | LV g | 20-40 | CD / 27.5 |
| Sw-5001-080-ir | CXXII g | 30-60 | CCCL / 24.0 |
| Sw-5001-120-ir | CLXXX g | 40-80 | CCC / 20.7 |
| Sw-5001-220-ir | CCCXL g | 50-100 | CCC / 20.7 |
| Sw-5001-330-ir | CDLXXV g | 50-100 | CCL / 17.2
|
Tabula II. Series Saxo Saxbs Sax Martridges. Stipare materiae: Ultra pura irregular Sax-Bonded Silica, XL - LXIII μm LXIII Â.
Nam ulteriores notitia in detailed cubits of Sepabean ™Machina, aut ordinatio notitia in Series Series Flash Cartridges, placere visita nos website.
Post tempus: Nov, 09-2018