Rui Huang, Bo Xu
Applicationem R & D Center
Introductio
A peptide composita composito ex amino acida, cuiusque cuius habet unique corporalis et eget proprietatibus debitum ad diversas genera et ordo amino acidum residuae constituting eius sequence. Cum progressionem solidum tempus eget synthesis, in chemical synthesis ex variis activae peptides fecit magni progressus. Tamen, debitum ad complicated compositionem peptide adeptus per solidum phase synthesis, in ultima uber debet purificari a certa separationem modi. Communiter usus PECUNDITIO modi ad Peptides includit Ion Exchange Chromatography (IEC) et Reversed-Phase High Ebrs Liquid Chromatography (RP-HPRIGENTIA PRESTENTUM EXPLORATIO PRIMUS SANDIDAE TECHTA, et EXPLICATION PRIMUS SANTAI TECHDOTAE ALTECTUM EXPLICATIONS ASPECTAE SANTAI technologies In quo a separacheflash rp c18 cartridge est usus ad celeri purificationem thymopentin (TP-V) et scopum uber occurrens in requisita adeptus est.
Figura I. XX communis amino acida (expressi a www.bachem.com).
Sunt XX genera amino acida, qui sunt communia in compositionem peptides. Hi amino acida potest dividitur in sequentibus coetibus secundum eorum verticitatem et acidum-basi res: Non-Suspendisse (Hydrophobic), Suspendisse (Unhorrged), acidic aut basic (ut ostensum est in Figura I). In peptide serie, si amino acida constituens sequentia sunt plerumque polaris ones (ut notatum in rosea color in Figura I), ut cysteine, tyrosine, et hunc peptids ut fortis polarity et hunc solutum aquam. Per Purificacionem procedure pro his fortis peptide exemplaria perversari-phase Chromatography, a phaenomenon dicitur Hydrophobic phase ruina erit (referre ad a antea published application note per Santai Technologies: Hydrophobic Phase Columnas, AQ Reversed Phase Columnas et Applications). C18 C18 comparari columnas melius C18aq columnas aptissima purificatione fortis Suspendisse aut hydrophilic exempla. Hoc post, fortis peptide erat usus est sample et purificatus a C18aq columna. Sicut effectus, in scopum productum occurrens requisita est adeptus potuit esse in sequentibus investigationis et progressionem.
| Instrumentum | Sepabean™Machina II | |||
| Cartridges | XII G Sepeflash C18 RP Flash Cartridge (Sphaerical Silica, XX - XLV μm, C å, Ordinis Nunber: Sw-5222-012, SP) | XII G Sepeflash c18aq RP Flash Cartridge (Sphaerical Silica, XX - XLV μm, C a, ut Number: Sw-5222-012, SP (AQ)) | ||
| RECONCINNUS | CCLIV NM, CCXX NM | CCXIV NM | ||
| Mobile Phase | Solvent A: Aqua Solvent B: Acathetonitrile | |||
| Rate fluunt | XV ml / min | XX ml / min | ||
| Sample loading | XXX mg | |||
| Clivus | Tempus (CV) | Solvent B (%) | Tempus (min) | Solvent B (%) |
| 0 | 0 | 0 | 4 | |
| 1.0 | 0 | 1.0 | 4 | |
| 10.0 | 6 | 7.5 | 18 | |
| 12,5 | 6 | 13.0 | 18 | |
| 16.5 | 10 | 14.0 | 22 | |
| 19.0 | 41 | 15,5 | 22 | |
| 21.0 | 41 | 18.0 | 38 | |
| / | / | 20.0 | 38 | |
| 22.0 | 87 | |||
| 29.0 | 87 | |||
Results et disputationem
Compare Purificacionis perficientur ad peptide specimenque inter iusto C18 columna C18AQ columna, utendum iusto C18 columna pro mico purificatione sample ut initium. Ut ostensum est in Figura II, debitum ad Hydrophobic Phase ruina C18 vincula per altum aqueum Ratio, sample vix retinuit in regularis C18 cartridge et directe elutu ex mobili tempus. Sicut effectus, in sample non efficaciter separata et purificatus.
Figura II. Et Flash chromatogram de sample in iusto C18 cartridge.
Deinde, utendum est C18AQ columnae ad Flash purificationem specimen. Ut ostensum est in Figura III, in peptide erat effectus retinuit in columna et elutari ex. Target productum separata ab impudicitiis in rudis sample et collecta. Post lyophillam et resolvitur per hplc, purificatum uber habet puritatem 98.2% et posset esse adhuc usus est proximus gradus investigationis et progressionem.
Figura III. Et Flash chromatogram de sample in c18aq cartridge.
In conclusione, Sepaflash c18aq RP Flash Cartridge combined cum Flash Chromatography System Sepabean™Machina potuit offerre ieiunium et effective solutio ad purificationem fortis Suspendisse aut hydrophilic exempla.
Sunt series in Sepaflash c18aq RP mico cartridges cum diversis specifications a Santai technology (ut ostensum est in Table II).
| Item numerus | Columna | Rate fluunt (M / min) | Max.pressure (Psi / Bar) |
| Sw-5222-004-SP (AQ) | 5.4 g | 5-15 | CD / 27.5 |
| Sw-5222-012, SP (AQ) | XX g | 10-25 | CD / 27.5 |
| Sw-5222-25-SP (AQ) | XXXIII g | 10-25 | CD / 27.5 |
| Sw-5222-040-SP (AQ) | XLVIII G | 15-30 | CD / 27.5 |
| Sw-5222-080-SP (AQ) | CV g | 25-50 | CCCL / 24.0 |
| Sw-5222-120, SP (AQ) | CLV g | 30-60 | CCC / 20.7 |
| Sw-5222-220-SP (AQ) | CCC g | 40-80 | CCC / 20.7 |
| Sw-5222-330-SP (AQ) | CDXX G | 40-80 | CCL / 17.2 |
Table II. Sepaflash c18aq RP mico cartridges. Stipare materiae, summus efficientia sphaerici C18 (AQ) -Bonded Silica, XX - μm XLV, C a.
Nam ulteriores notitia in detailed cubits of Sepabean ™ apparatus, aut ordinatio notitia in Series Series Cartridges, placere visitare noster website.
Post tempus: Oct-12-2018