Tiiyuan Qan, Yuefeng Tan, Bo x
Ngwa R & D
Ndubata
Taxtus (tagsus chinensis ma ọ bụ ndị China yEW) bụ osisi ọkụkụ na-echebe site na mba ahụ. Ọ bụ osisi dị ụkọ ma nke ahụ mebiri emebi hapụrụ site na glociers. Ọ bụkwa naanị ọgwụ ọgwụ eke ụwa. A na-ekesa tazis na mpaghara dị iche iche nke ugwu ọwụwa anyanwụ na mpaghara nke subropical, ihe dị ka otu 11 n'ụwa. E nwere ụdị 4 na 1 dị iche iche na China, onye na - abụghị Takus na ugwu Yunnan, Takus, Taxtus Taxtus ndịda. A na-ekesa ụdị ise ise ndị a na Southwest China, South China, Central China, East China, Northwest Sundan na Taiwan na Taiwan. Mkpụrụ osisi taxus nwere ọtụtụ ihe dịgasị iche iche, gụnyere ụtụ, flavoids, lanins, stenalics, phenalic acs, phenilic acid, susquiterpenes na glycosides. Ndị a ma ama anti-tumol ọgwụ (ma ọ bụ Paclipaxel) bụ ụdị taktan. Takiol nwere usoro ọgwụ pụrụ iche anticticr. Taktọl nwere ike "ifriizi" microtubules site na ijikọta ha ma gbochie microtubules site na cell cell nke na-ekewapụ mkpụrụ ndụ, ọkachasị na-eto eto mkpụrụ ndụ kansa kansa [1]. Ọzọkwa, site na-arụ ọrụ macrophges, tagon na-eme ka mbelata tnf-α (tumosis nevrosis nke), site na igbu mmadụ ma ọ bụ gbochie mkpụrụ ndụ tumo. Ọzọkwa, taxol nwere ike ịde ọgwụ apoptosis site na ime ihe autoptoutic na-eme ka ọ dị na FAS / FASL ma ọ bụ rụọ ọrụ cysteine proto na-emetụ elu [3]. N'ihi ọtụtụ lekwasịrị anya na-egbochi ihe na-egbochi ya, cancer ọrịa cancer, ọrịa cancefan, onye isi na cancer cancer, wdg [4]. Karịsịa maka ọrịa cancer dị elu na ọrịa kansa dị elu, ya mere a maara ya dị ka "eriri ikpeazụ nke ịgbachitere maka ọgwụgwọ kansa".
Akwa a ọgwụ ọjọọ bụ ọgwụ kachasị ewu ewu na mba ofesi na afọ ndị na-adịbeghị anya ma na-ahụta na ọ bụ otu n'ime ọgwụ anticancer maka ụmụ mmadụ n'afọ iri abụọ sochirinụ. N'afọ ndị na-adịbeghị anya, na uto nke ndị bi na ọrịa kansa, ọchịchọ maka Tagol enwewokwa nke ukwuu. Ugbu a, taglol chọrọ maka nyocha ma ọ bụ nke sayensị na-ewepụta ngwa ngwa site na taketus. Ọ bụ ihe nwute, ọdịnaya nke tagon na osisi dị ala. Dịka ọmụmaatụ, ọdịnaya tagon bụ naanị 0.069% na ogbugbo nke tagsus Brevifolia, nke a na-ewere dị ka ọdịnaya kachasị elu. Maka mmịpụta nke 1 g nke taktol, ọ chọrọ ihe dị ka 13.6 n'arọ nke taxus ogbugbo. Dabere na atụmatụ a, ọ ga-ewe 3 osisi tazis nke karịrị otu narị afọ iji gwọọ ọrịa kansa ovaan. N'ihi ya, egbutuwo ọtụtụ osisi ụtụ isi, na-ebute ikpochapụ mgbe a dị oke ọnụ ahịa. Na mgbakwunye, takrus dara ogbenye na akụrụngwa ma na-egbu oge na uto, nke na-eme ka o sie ike maka mmepe na itinye tagon.
Ugbu a, mkpokọta usoro nke Taxol rụzuru nke ọma. Agbanyeghị, ụzọ synity ya siri ike ma dị elu, na-eme ka ọ nweghị ezigbo ọrụ. Usoro ngagharị nke taxol ugbu a ma na-ahụta ka ọ bụrụ ụzọ dị mma iji gbasaa isi mmalite nke taxOl na mgbakwunye na akuku. Na nkenke, na ọkara-syntesis nke tagon, a na-ebu ụzọ tagon nke dị ukwuu nke ukwuu na osisi dị ukwuu ma gbanwee ya na taxtus nke kemịkalụ. Ọdịnaya nke 10-deaegtyCatin ⅲ Na mkpa nke taggus baccata nwere ike ruo 0.1%. Na mkpa ndị ahụ dị mfe ịmaliteghachi na ogbugbo. Ya mere, njikọta nke taktọ dabere na 10-deaegtyckatin ⅲ na-adọtakwu ntị karịa na ndị nyocha [5] (dị ka egosiri na eserese 1).
Ọgụgụ 1
Na post a, igwe na-emepụta ihe eji eme ihe na-eme ka ihe na-eme ka sepabeean ™ dị na Sepaflash c18 tụgharịrị-flash Cartrities mepụtara. Nzukọ ngwaahịa na-ezute a na-eme ka a nweta ihe a chọrọ na nyocha sayensị, na-enye ihe dị ọnụ ahịa maka ụdị ngwaahịa a eke.
| Nkwa egwu | Igwefoto SEPASEAN ™ | |
| Mkpoegbe | 12 g SepaFlash C18 RP flash cartridge (spherical silica, 20 - 45μm, 100 Å, Order number：SW-5222-012-SP) | |
| Evel | 254 nm (nchọta), 280 nm (nlekota) | |
| Mobile | Belvent a: Mmiri | |
| Belvernt B: Methanol | ||
| Ọnụego asọmpi | 15 ml / min | |
| Ntinye uche | Elekere 20 mg na-agbaze na 1 ml DMSO | |
| Kenleda anya | Oge (min) | Belvent b (%) |
| 0 | 10 | |
| 5 | 10 | |
| 7 | 28 | |
| 14 | 28 | |
| 16 | 40 | |
| 20 | 60 | |
| 27 | 60 | |
| 30 | 72 | |
| 40 | 72 | |
| 43 | 100 | |
| 45 | 100 | |
Nsonazụ na mkparịta ụka
E gosipụtara na flash chromatogram maka ndọtị ahụ sitere na taxus na eserese nke abụọ. Site na nyocha chromatogram, ngwaahịa a na-ahụ maka ebule na-enwe nkewa. Ọzọkwa, ezigbo ịmụgharị anya bụ ezigbo ngọngọ (data egosighi). Ọ ga-ewe ihe dị ka awa anọ iji mezue nkewa na usoro plamatography na kọlụm iko. Itinye ya na usoro iwu chromatation, usoro ọcha akpaka na post a na-achọ nkeji 44 iji mezue ọrụ dị ọcha (dị ka egosiri na eserese 3). Enwere ike ịchekwa ihe karịrị 80% nke oge na nnukwu ego ga-azoputa site na iwere akpaka, nke nwere ike belata ọnụ ahịa ya nke ọma.
Ọgụgụ 2
Ọgụgụ 3. Tụlee ntụle nke usoro chromatography na arụmọrụ ọcha akpaka.
Na mmechi, jikọtara Sepaflash c18 RP cartridgeges na igwe Sepaber nwere ike inye ngwa ngwa na arụmọrụ ngwa ngwa maka ngwa ngwa nke anụ ahụ dịka wepụta taxtus.
Ebe zoro aka
1. Alushin GM, Verher GC, Kellegg Eh, Baker R na Ngales D na Ngale Ngwá Ọrụ na-ekpughe ntụgharị usoro na αβ-tublin na gtp hydrolysis. Cell, 2014, 157 (5), 1117-1129.
2. Burkhart Ca, Berman JW, Swindell CS na Horwitz SB. Mmekọrịta dị n'etiti usoro taxol na ndị ọzọ na-akwụ ụtụ na ntinye nke tumosis-α dee na sey cytooxication. Nnyocha cancer, 1994, 54 (22), 5779-5782.
3. Park sj, WU ch, Gordon JD, Zhong x, emami a na saga a. Taktol na-emepụta caspase-10-dabere na apoptosis, j. biol. Chem., 2004, 279, 51057-51067.
4. Paclipaxel. Ndị America Society nke ndị na-ere ọgwụ na-ahụ maka ahụike. [Jenụwarị 2, 2015]
5. Bruce Ganem na Roland R. Franke. PacLimaxel si tagnan tagnan: echiche na ihe okike na Sanko Kwaozictonium Chemistry. J. org. Chem., 2007, 72 (11), 3981-3987.
Enwere usoro nke Sepaflash c18 RP cartridges nwere nkọwa dị iche iche sitere na teknụzụ Santai (dịka egosiri na Isiokwu 2).
| Nọmba ihe | Ihu kọlụm | Ọnụego asọmpi (ml / min) | Max.pressure (PSI / OR) |
| SW-5222-004-sp | 5.4 g | 5-15 | 400 / 27.5 |
| SW-5222-012-sp | 20 g | 10-25 | 400 / 27.5 |
| SW-5222-025-sp | 33 g | 10-25 | 400 / 27.5 |
| SW-5222-040-sp | 48 g | 15-30 | 400 / 27.5 |
| SW-5222-080-sp | 105 g | 25-50 | 350 / 24.0 |
| SW-5222-120-SP | 155 g | 30-60 | 300 / 20.7 |
| SW-5222-220-SP | 300 g | 40-80 | 300 / 20.7 |
| SW-5222-330-sp | 420 g | 40-80 | 250 / 17.2 |
Tebụl 2. Sepaflash c18 RP cartridges.
Ihe ejikọtara ọnụ: arụmọrụ dị elu silica, 20 - 45 μm, 100)
Maka ozi ndị ọzọ na ngwanrọ zuru ezu nke igwe Spabe nke Sepabea, ma ọ bụ ozi na-atụ n'usoro Sepaflash Carridges, biko gaa na websaịtị anyị
Oge Post: Sepp-20-2018