Mwepụta Taxus dị ọcha site na igwe SepaBean™

Meiyuan Qian, Yuefeng Tan, Bo Xu
Ngwa R&D Center

Okwu mmalite
Taxus (Taxus chinensis ma ọ bụ Chinese yew) bụ ahịhịa ọhịa nke obodo na-echebe.Ọ bụ osisi na-adịghị ahụkebe na nke dị ize ndụ nke glaciers Quaternary hapụrụ.Ọ bụkwa naanị osisi ọgwụ okike n'ụwa.A na-ekesa taxus na mpaghara okpomọkụ nke ugwu ugwu ruo n'etiti mpaghara subtropical, nwere ihe dị ka ụdị 11 n'ụwa.Enwere ụdị 4 na 1 dị iche iche na China, ya bụ Northeast Taxus, Yunnan Taxus, Taxus, Tibet Taxus na Southern Taxus.A na-ekesa ụdị ise a na Southwest China, South China, Central China, East China, Northwest China, Northeast China na Taiwan.Osisi taxus nwere ụdị kemịkal dị iche iche, gụnyere taxes, flavonoids, lignans, steroid, phenolic acid, sesquiterpenes na glycosides.Ọgwụ mgbochi tumor a ma ama Taxol (ma ọ bụ Paclitaxel) bụ ụdị ụtụ isi.Taxol nwere usoro mgbochi ọrịa kansa pụrụ iche.Taxol nwere ike "friza" microtubules site na ijikọta ha ma gbochie microtubules si na-ekewa chromosomes n'oge nkewa cell, si otú a na-eduga na ọnwụ nke na-ekewa mkpụrụ ndụ, karịsịa ngwa ngwa na-amụba mkpụrụ ndụ cancer[1].Ọzọkwa, site na ịgbalite macrophages, Taxol na-eme ka mbelata nke TNF-α (tumor necrosis factor) ndị na-anabata ya na ntọhapụ nke TNF-α, si otú ahụ na-egbu ma ọ bụ na-egbochi mkpụrụ ndụ tumo [2].Ọzọkwa, Taxol nwere ike ịkpalite apoptosis site n'ime ihe na ụzọ nnabata apoptotic nke Fas / FasL na-agbaziri ma ọ bụ mee ka usoro cysteine ​​protease [3] rụọ ọrụ.N'ihi mmetụta anticancer ya dị iche iche, a na-eji Taxol eme ihe n'ọtụtụ ebe n'ịgwọ ọrịa cancer ovarian, ọrịa ara ara, ọrịa cancer akpa ume na-abụghị obere cell (NSCLC), kansa gastric, ọrịa kansa esophageal, kansa eriri afọ, kansa prostate, melanoma ọjọọ, isi na olu. ọrịa cancer, wdg[4].Karịsịa maka ọrịa ara ara dị elu na ọrịa cancer ovarian dị elu, Taxol nwere mmetụta ọgwụgwọ pụtara ìhè, ya mere a maara ya dị ka "usoro nchebe ikpeazụ maka ọgwụgwọ ọrịa cancer".

Taxol bụ ọgwụ mgbochi ọrịa kansa kacha ewu ewu n'ahịa mba ụwa n'afọ ndị na-adịbeghị anya, a na-ewere ya dị ka otu n'ime ọgwụ mgbochi ọrịa kansa kacha mma maka ụmụ mmadụ n'ime afọ 20 na-esote.N'afọ ndị na-adịbeghị anya, site na mmụba na-agbawa agbawa nke ọnụ ọgụgụ mmadụ na ọrịa cancer, ọchịchọ Taxol abawanyela nke ukwuu.Ugbu a, Taxol chọrọ maka nyocha ụlọ ọgwụ ma ọ bụ sayensị na-esite na Taxus ozugbo.N'ụzọ dị mwute, ọdịnaya nke Taxol na osisi bụ nnọọ ala.Dịka ọmụmaatụ, ọdịnaya Taxol bụ naanị 0.069% na ogbugbo Taxus brevifolia, nke a na-ewerekarị na ọ nwere ọdịnaya kachasị elu.Maka mmịpụta nke 1 g Taxol, ọ chọrọ ihe dị ka kilogram 13.6 nke ogbugbo Taxus.Dabere na atụmatụ a, ọ na-ewe 3 – 12 osisi Taxus nke dị ihe karịrị 100 afọ iji gwọọ onye ọrịa cancer ovarian.N'ihi ya, e gbutuola ọnụ ọgụgụ dị ukwuu nke osisi Taxus, na-eme ka ọ dị nso na ikpochapụ maka ụdị a dị oké ọnụ ahịa.Ke adianade do, Taxus bụ nnọọ ogbenye na akụrụngwa na ngwa ngwa na-eto eto, nke na-eme ka o siri ike n'ihu mmepe na itinye n'ọrụ nke Taxol.

Ugbu a, mkpokọta mkpokọta Taxol emechaala nke ọma.Otú ọ dị, ụzọ sịntetik ya dị mgbagwoju anya na ọnụ ahịa dị elu, na-eme ka ọ ghara inwe mkpa ụlọ ọrụ.Usoro Taxol nke ọkara synthetic dị ugbu a tozuru oke ma a na-ewere ya dị ka ụzọ dị irè isi gbasaa isi mmalite Taxol na mgbakwunye na ịkụ ihe ọkụkụ.Na nkenke, na ọkara njikọ nke Taxol, a na-amịpụta Taxol precursor compound nke dị oke ụba na osisi Taxus wee ghọọ Taxol site na njikọ kemịkalụ.Ọdịnaya nke 10-deacetylbaccatin Ⅲ na agịga Taxus baccata nwere ike iru 0.1%.Na agịga dị mfe ịmalite iji tụnyere ogbugbo.Ya mere, ọkara njikọ nke Taxol dabere na 10-deacetylbaccatin Ⅲ na-adọtakwu ndị na-eme nchọpụta [5] (dị ka egosiri na Figure 1).

Ọgụgụ 1. Ụzọ ọkara synthetic nke Taxol dabere na 10-deacetylbaccatin Ⅲ.

Na post a, a na-asachapụ ihe ọkụkụ Taxus site na sistemu chromatography mmiri na-akwadebe ngwa ngwa SepaBean ™ yana ngwakọta SepaFlash C18 reversed-phase (RP) flash katrij nke Santai Teknụzụ mepụtara.E nwetara ngwaahịa e lekwasịrị anya na-ezute ihe ndị dị ọcha chọrọ ma nwee ike iji ya na nyocha sayensị na-esote, na-enye ihe ngwọta dị ọnụ ahịa maka ịdị ọcha ngwa ngwa nke ụdị ngwaahịa eke.