RUI Huang, Bo x
Ngwa R & D
Ndubata
Atino acid mejupụtara amino acid, nke ọ bụla nwere ihe pụrụ iche anụ ahụ na kemịkal dị iche iche na usoro acid acid na-agụ usoro ya. Site na mmepe nke usoro kemịkal nke ọma, na kemịkalụ nke peptide dị iche iche emeela ka nnukwu ọganihu. Agbanyeghị, n'ihi ihe mgbagwoju anya nke peptide nke nwetara site na njikọta nke ngwa siri ike, ngwaahịa ikpeazụ kwesịrị ka ọ dị ọcha site na ụzọ nkewapụrụ iche. A na-ejikarị usoro a na-eme ihe eji eme ihe eji eme ihe na-agbanwe agbanwe - Ion) na akụrụngwa nke obere moleCography, wdg <1 KDA), Santai bipụtara na mbụ site na Santai A na-enweta teknụzụ sepaflash R18.
Ọgụgụ 1
Enwere ụdị amino acid iri abụọ nke bụ ihe a na-ahụkarị na nchịkọta nke peptides. Enwere ike kee amino acid na ndị na-eso ya dị ka ihe ndị na-eso ya dị na ngalaba acid: na-abụghị pol (hydrophobic), polar (dị ka ihe atụ (dị ka egosiri). N'ime usoro peptino, ma ọ bụrụ na amino acid na-agụchi usoro ahụ bụ nke kachasị mma (dị ka akara Cysteine, Cyreonine, wdg mgbe ahụ ka ọ ga-enwe ike na mmiri. N'oge usoro dị ọcha nke poptide ndị a siri ike site na resed-spraphotography, phenomenon a na-akpọ hydrophobic flative flate na mbụ, aq sprap mode, AQ gbanwere kọmịshọm chrase na ngwa ha). E jiri ya tụnyere kọlụm C18 mgbe nile, kọlụm C1AQ kachasị mma dabara adaba maka ọcha nke ike polar polar ma ọ bụ ihe nlere nke hydrophilic. Na post a, a na-eji polar siri ike poptide dị ka ihe nlele ma dị ọcha site na C1AQ kọlụm. N'ihi ya, a na-enweta ihe ndị a chọrọ iji mezue ihe ndị a chọrọ ma nwee ike iji ya na nyocha na mmepe.
| Nkwa egwu | Ozabia™igwe 2 | |||
| Ugbo ala | 12 g Sepaflash c18 RP FL FLAVED (SPEHACE SILA, 20 - 45 μm, 100), Ruo-5222-012-SP) | 12 g Sepaflash c18aq rp clash clash clash clash clash clash clash, 20 - 10022-012-012-sp (AQ)) | ||
| Evel | 254 nm, 220 nm | 214 NM | ||
| Mobile | Belvent a: Mmiri Belvent B: acetonitrael | |||
| Ọnụego asọmpi | 15 ml / min | 20 ml / min | ||
| Ntinye uche | 30 mg | |||
| Kenleda anya | Oge (CV) | Belvent b (%) | Oge (min) | Belvent b (%) |
| 0 | 0 | 0 | 4 | |
| 1.0 | 0 | 1.0 | 4 | |
| 10.0 | 6 | 7.5 | 18 | |
| 12.5 | 6 | 13.0 | 18 | |
| 16.5 | 10 | 14.0 | 22 | |
| 19.0 | 41 | 15.5 | 22 | |
| 21.0 | 41 | 18.0 | 38 | |
| / | / | 20.0 | 38 | |
| 22.0 | 87 | |||
| 29.0 | 87 | |||
Nsonazụ na mkparịta ụka
Iji tulee arụmọrụ nke popira na kọlụm C18 mgbe niile na kọlụm C18aq mgbe niile, anyị ji kọlụm C18 maka kọlụm Flash nke ihe nlele ahụ dị ka mmalite. Dị ka egosiri na eserese nke abụọ, n'ihi usoro a na-eme ka ọ dị elu nke osisi C18 kpatara nke ọma, a na-ejigide ihe nlele ahụ na oge C18 mgbe niile ma na-akpọpụta crase Cardidge mgbe niile ma kpochapụ ya site na ekwentị mkpanaaka. N'ihi ya, ewezugaghị ahụ nke ọma ma dị ọcha.
Ọgụgụ 2
Ọzọ, anyị jiri kọlụm C18AQ maka Flash ọcha nke ihe nlele ahụ. Dị ka egosiri na eserese nke 3, a na-ejide peptide nke ọma na kọlụm ahụ wee kpochapụ. A na-ekewa ngwaahịa ndị zubere na adịghị mma na ntuli aka ma na-anakọta. Mgbe ị ga - eleghachi anya site na Hplc, ngwaahịa dị ọcha nwere ịdị ọcha nke 98.2% ma nwee ike ijikwa ya maka nyocha na mmepe na mmepe.
Ọgụgụ 3
Na mmechi, sepaflash c18aQ rp cartridge jikọtara ya na usoro flash chromatography sylabea™Igwe igwe nwere ike inye ihe ngwọta ngwa ngwa ma dị irè maka ịdị ọcha polar polar ma ọ bụ ihe nlele.
Onwere usoro sepaflash c18aq rp cartridges nwere nkọwa dị iche iche sitere na teknụzụ Santai (dị ka egosiri na Isiokwu 2).
| Nọmba ihe | Ihu kọlụm | Ọnụego asọmpi (ml / min) | Max.pressure (PSI / OR) |
| SW-5222-004-SP (AQ) | 5.4 g | 5-15 | 400 / 27.5 |
| SW-5222-012-SP (AQ) | 20 g | 10-25 | 400 / 27.5 |
| SW-5222-025-SP (AQ) | 33 g | 10-25 | 400 / 27.5 |
| SW-5222-040-SP (AQ) | 48 g | 15-30 | 400 / 27.5 |
| SW-5222-080-SP (AQ) | 105 g | 25-50 | 350 / 24.0 |
| SW-5222-120-SP (AQ) | 155 g | 30-60 | 300 / 20.7 |
| SW-5222-220-SP (AQ) | 300 g | 40-80 | 300 / 20.7 |
| SW-5222-330-SP (AQ) | 420 g | 40-80 | 250 / 17.2 |
Tebụl 2. Sepaflash c18aQ RP Cartridges. Ngwakọta na - ebukọta: arụmọrụ dị elu C18 (AQ) - na - enye silica, 20 - 45 μm, 100.
Iji nwetakwuo ihe ọmụma banyere igwe zuru ezu nke igwe Spabe nke Sepabiean ™, ma ọ bụ ozi ịtụ maka cartided carridges, biko gaa na websaịtị anyị.
Oge post: Ọkt-12-2018