Hongchang Wang, bo XU
Ngwa R & D
Ndubata
Dabere na Polaties nke ekwentị nke ekwentị na ekwentị, mmiri chromatography na nkịtị was chromatography (NPC) ma tụgharịa usoro warara (RPC). Maka rpc, polarality nke ekwentị mkpanaka siri ike karịa nke usoro ọdụ. RPC aghọọla otu ihe kachasị eji eme ihe na mpaghara Chromatography n'ihi ịdị elu ya dị elu, mkpebi dị mma na usoro ndozi doro anya na usoro njigide. Ya mere rpc kwesịrị ekwesị maka nkewa na okpomọkụ nke polar dị iche iche ma ọ bụ nke acidio, acidii, na-eme nke ọma, na-eme ihe na-arụ ọrụ dị iche iche, gụnyere C18, C8, Phenyl, Cyano, Amino, wdg, wdg. N'ime otu ọrụ ndị a na-arụ ọrụ, otu otu bụ otu bụ C18. A na-eme atụmatụ na ihe karịrị 80% nke RPC na-eji ugbu a na-adọta c18. Ya mere kọlụm C18 Chromatography abụrụla otu kọlụm maka ụwa niile.
Ọ bụ ezie na enwere ike iji kọlụm C18 n'ọtụtụ ngwa nke ngwa dị iche iche, maka ụfọdụ ihe nlele nke dị oke mma polar ma ọ bụ kọlụm c1Drophic, nwere ike iji ya mee ihe mgbe a na-eme ya. Na rpc, enwere ike inye iwu nke ọma dị ka porarity si dị: Mmiri <methanol <ethanol <Tetrahydroful <inopropanol <thapropananol <inopropanol <inopropananol <emopropanol <imopropanol <thapropanol <thapropanol <thapropanol <thapropanol <thapropanol <thapropananol <etropropanol Iji mesie ezigbo nsogbu na kọlụm maka ihe nlele ndị a (polar siri ike ma ọ bụ nke ukwuu hydrophilic), dị elu nke usoro ihe ọghọm dị mkpa iji mee ihe dị ka ekwentị. Agbanyeghị, mgbe ị na-eji usoro mmiri dị ọcha (gụnyere mmiri dị ọcha ma ọ bụ mmiri dị ọcha) na kọlụm nke C18 kọlụm, na-ebute na ibe ya na njikọta ma ọ bụ enweghị nchedo. A na-akpọ a phenomenon "hydrophobic usoro" (dị ka egosiri na akụkụ aka ekpe nke ọgụgụ 1). Ọ bụ ezie na ọnọdụ a na-atọghachi ya mgbe a na-eji ihe na-edozi ahịhịa dị ka methanol ma ọ bụ acetonitrile, ọ ka nwere ike imebi ihe kọlụm. Ya mere, ọ dị mkpa iji gbochie ọnọdụ a.
Ọgụgụ 1
Iji lebara nsogbu ndị ae kwuru n'elu, ihe ndị na-emepụta chromatographics emepụtala nkwalite ọrụ. Otu n'ime ndozi ndị a na-eme ka ụfọdụ mgbanwe dị n'elu elu nke silica matriks, dị ka ngosipụta nke hydrophilic Conse (dị ka egosiri na akụkụ aka nri nke eserese 1), iji mee ka elu silica gel karịa hydrophilic. N'ihi ya, a ga-eji eriri C18 dị na elu silica nwere ike ịgbatị kpamkpam n'okpuru ala a na-adabara na ndagwurugwu hydrophobic nke a ga-ezere. A na-akpọ kọlụm C18 kọlụm, ya bụ kọlụm c18aQ, nke a na-eme maka usoro ihe omimi ma nwee ike ịnagide 100%. A na-etinye kọlụm C18aQ ebe niile na nkewa nke ogige siri ike, gụnyere ihe acids, peptides, nucleosides na mmiri soluble.
Ihe na-egbochi ya bụ otu n'ime ngwa nke C18A na-eme ka ọ dị ọcha na-ekpo ọkụ maka ihe nlele, nke na-ewepụ nnu ma ọ bụ ihe nlele iji kwado ngwa nke ihe nlele ahụ. Na post a, ejiri polarity na-acha anụnụ anụnụ dị ka ihe nlele ma dị ọcha na kọlụm C18AQ. Ejiri akụkụ sochiri anya sitere na njikwa nchekwa, si otú a na-eme ka ndị na-eso ụzọ Rory na-eme ka ihe mgbapụta na oge nchekwa. Ọzọkwa, ịdị ọcha nke ihe nlele ahụ ka mma site na iwepu adịghị adịghị mma na nlele ahụ.
Ngalaba nyocha
Ọgụgụ 2. Na kemịkalụ nke ihe nlele ahụ.
Ejiri FCF na-acha anụnụ anụnụ FCF dịka ọmụmaatụ na post a. E gosipụtara ịdị ọcha nke ihe nlereanya ahụ dị 86% na usoro kemịkalụ nke ihe nlele ahụ, iji kwadebe ngwọta na-acha anụnụ anụnụ na-acha odo odo na-acha uhie uhie na-acha odo odo ma mee ka ọ bụrụ nke ọma ka ọ bụrụ ihe doro anya. Emere ihe nlele ahụ n'ime kọlụm flash site na injector. Edepụtara usoro nnwale nke Flash ọcha dị na tebụl 1.
| Nkwa egwu | Igwefoto SEPASEAN ™2 | |||
| Ugbo ala | 12 g Sepaflash c18 RP FL FL FLAVE (SPEHACE SILICA, 20 - 45 μm, 100222-012-SP) | 12 g Sepaflash c18aq rp clash clash clash clash clash clash clash (silica 5222-012-SP (AQ)) | ||
| Evel | 254 nm | |||
| Mobile | Belvent a: Mmiri Belvernt B: Methanol | |||
| Ọnụego asọmpi | 30 ml / min | |||
| Ntinye uche | 300 mg (amamiihe acha anụnụ anụnụ fcf na ịdị ọcha nke 86%) | |||
| Kenleda anya | Oge (CV) | Belvent b (%) | Oge (CV) | Belvent b (%) |
| 0 | 10 | 0 | 0 | |
| 10 | 10 | 10 | 0 | |
| 10.1 | 100 | 10.1 | 100 | |
| 17.5 | 100 | 17.5 | 100 | |
| 17.6 | 10 | 17.6 | 0 | |
| 22.6 | 10 | 22.6 | 0 | |
Nsonazụ na mkparịta ụka
Ejiri sepaflash c18aq rp clash cartriddid maka ileba anya na ime ka ọ dị ọcha. A na-eji ihe na-emesi ihe ike mee ihe eji eme mmiri dị ka oge ekwentị na mmalite nke ịtụ aro ma na-agba ọsọ maka mpịakọta 10 kọlụm (CV). Dị ka egosiri na eserese 3, mgbe ị na-eji mmiri dị ọcha dị ka ekwentị mkpanaaka, nlele ahụ na-ejide kpamkpam na katrik flash. Na-esote, Methanol dị na ekwentị mkpanaka ka mụbaa 100% na ahụ gradied ahụ maka 7.5 CV. A na-esite na nlele ahụ pụta site na 11.5 ruo 13.5 CV. Na irighiri ihe ndị na-atọkọta, a na-edochi ihe ga-anọchi usoro nchekwa nah2pol. Iji ya atụnyere nke dị elu, methanol dị mfe ka ewepu ya na nzacha rotari na nzọụkwụ ndị ọzọ, nke na-eme ka nyocha a.
Ọgụgụ 3
Iji tụnyere omume njigide nke C18aq na c18 cartridge mgbe niile maka ihe nlere nke polarity, a rụrụ ule ntụle myirịta. A na-eji sepaflash c18 RP Clash Clash Clash Trash Chromatogram maka ihe nlereanya ahụ na eserese 4 Ya mere, mmalite ahụ dị na 10% methanol na mmiri 90%. Dị ka egosiri na eserese 4, n'ihi usoro nke C18 nke agbụ nke osisi C18 kpatara nke ọma, a na-ejigide ihe nlele ahụ na ekwentị C18 mgbe niile ma kpochapụ ya site na ekwentị mkpanaaka. N'ihi nke a, a gaghị emecha ọrụ nke ileba anya ma ọ bụ ịdị ọcha.
Ọgụgụ 4
Iji ya atụnyere iji Gleartient Linent, iji ihe na-esonụ nwere uru ndị a:
1
2. Ihe ndị a na-eleba anya na ọnụahịa dị nkọ, nke na-ebelata oke olu nke irighiri irighiri irighiri ihe ma na-eme ka oge nchekwa na-esochi ya.
3. Ngwaahịa anakọtara dị na methananol nke dị mfe a ga - ewepụ, yabụ na - ebelata oge ịla.
Na mmechi, maka udiri ihe nlereanya nke ihe nlere nke siri ike polar ma ọ bụ nke ukwuu hydrophilic, sepaflash cartring system cresh Clash Clash cart Sepabey Cell nwere ike inye ihe ngwa ngwa ma rụọ ọrụ nke ọma.
Banyere Sepaflash Boostem Form C18 RP Cartridges
Onwere usoro sepaflash c18aq rp cartridges nwere nkọwa dị iche iche sitere na teknụzụ Santai (dị ka egosiri na Isiokwu 2).
| Nọmba ihe | Ihu kọlụm | Ọnụego asọmpi (ml / min) | Max.pressure (PSI / OR) |
| SW-5222-004-SP (AQ) | 5.4 g | 5-15 | 400 / 27.5 |
| SW-5222-012-SP (AQ) | 20 g | 10-25 | 400 / 27.5 |
| SW-5222-025-SP (AQ) | 33 g | 10-25 | 400 / 27.5 |
| SW-5222-040-SP (AQ) | 48 g | 15-30 | 400 / 27.5 |
| SW-5222-080-SP (AQ) | 105 g | 25-50 | 350 / 24.0 |
| SW-5222-120-SP (AQ) | 155 g | 30-60 | 300 / 20.7 |
| SW-5222-220-SP (AQ) | 300 g | 40-80 | 300 / 20.7 |
| SW-5222-330-SP (AQ) | 420 g | 40-80 | 250 / 17.2 |
Tebụl 2. Sepaflash c18aQ RP Cartridges.
Ngwakọta na - ebukọta: arụmọrụ dị elu C18 (AQ) - na - enye silica, 20 - 45 μm, 100.
logy (dị ka egosiri na tebụl 2).
Oge Post: Ọgọst-27-2018