Mingzu Yang, Bo Xu
Daim Ntawv Thov R & D Center
Lub hauv paus rau phau ntawv
Cov tshuaj tua kab mob yog chav kawm ntawm cov metabolites thib ob (suav nrog cov kab mob (suav nrog cov kab mob sib xyaw) lossis cov khoom siv tshuaj uas zoo sib xws uas ua rau muaj kev sib xyaw. Cov tshuaj tua kab mob tuaj yeem ua rau muaj kev loj hlob thiab muaj sia nyob ntawm lwm cov kab mob me me. Thawj cov tshuaj tua kab mob tau tshawb pom los ntawm tib neeg, penicillin, tau pom los ntawm British microbiologist yws yws tias cov pwm tsis tuaj yeem cog rau hauv cov pwm. Nws tau tshaj tawm tias cov pwm yuav tsum tso tawm cov tshuaj tua kab mob, uas nws tau hu ua penicillin xyoo 1928. Txawm li cas los xij, cov khoom xyaw nquag tsis huv lub sijhawm ntawd. Xyoo 1939, lub pob zeb tshiab thiab Howard Florey ntawm Oxford University txiav txim siab tsim cov tshuaj uas tuaj yeem kho tus kab mob. Tom qab hu rau Fleming kom tau txais cov leeg, lawv tau ua tiav thiab huv penicillin los ntawm cov leeg. Rau lawv txoj kev loj hlob ntawm kev ua yeeb yam ntawm cov tshuaj zoo li cov tshuaj kho, khav, saw thiab florey qhia xyoo 1945 Nobel khoom plig hauv cov tshuaj.
Cov tshuaj tua kab mob yog siv los ua cov sawv cev rau cov neeg sawv cev ua kom kho lossis tiv thaiv kab mob. Muaj ntau ntau pawg ntawm cov tshuaj tua kab mob siv ua cov tshuaj tua kab mob tua kab mob, thiab lwm yam tshuaj tua kab mob, thiab lwm yam tshuaj tua kab mob muaj yees suav nrog kev siv tshuaj tua kab mob semi-synthesis thiab tag nrho synthesis. Cov tshuaj tua kab mob tsim tawm los ntawm kev hloov kho tsiaj txhu muaj kev hloov kho los ntawm kev siv tshuaj lom neeg vim muaj tshuaj ruaj khov, tshuaj lom thiab lwm yam teeb meem. Tom qab tshuaj hloov kho, cov tshuaj tua kab mob yuav ua tiav cov kev mob tawm tsam ntau ntxiv, txhim kho cov tshuaj tiv thaiv kab mob kom tsawg, txhim kho cov tshuaj tiv thaiv kab mob kom tsawg, thiab yog li txhim kho cov nyhuv tshuaj kho mob. Yog li ntawd, cov tshuaj tua hluav taws xob synthetic tam sim no yog tam sim no nrov tshaj plaws hauv kev txhim kho cov tshuaj tua kab mob.
Hauv kev txhim kho ntawm cov tshuaj tua hluav taws xob ib nrab, tshuaj tua kab mob muaj cov khoom siv ntawm cov khoom siv qis, ntau yam khoom siv tau los ntawm cov khoom lag luam fermial fermotation. Hauv qhov no, kev soj ntsuam thiab tswj cov impurities nyob rau hauv semi-synthetic cov tshuaj tua kab mob yog tshwj xeeb yog ib qho tseem ceeb. Txhawm rau kom paub zoo thiab yam ntxwv tsis txaus ntseeg, nws yog qhov tsim nyog kom tau txais cov khoom siv hluav taws xob ntawm cov khoom hluavtaws ntawm cov tshuaj tua kab mob syntheticics. Ntawm cov feem ntau siv cov txheej txheem npaj npaj los txawv, nyem cov qauv chromatography, tus nqi tsawg dua xws li cov kws tshawb fawb hluavtaws loj dua.
Hauv cov ntawv tshaj tawm no, lub ntsiab tseem ceeb ntawm cov tshuaj lom hluavtaws aminoglycoscoside tau siv los ua tus qauv thiab lub carromium chromatography sepabean ™. Lub hom phiaj khoom sib ntsib txoj cai tau ua tiav tau txais, qhia txog kev daws teeb meem zoo heev rau kev ua kom huv ntawm cov tebchaw no.
Kev sim ntu
Cov qauv tau ua siab zoo muab los ntawm lub tuam txhab chaw muag tshuaj hauv ib cheeb tsam. Cov qauv yog ib hom amino polyciClic carbohydrates thiab nws cov qauv molecular tau zoo sib xws nrog aminoglycoside tshuaj tua kab mob. Cov polarity ntawm cov qauv yog theej siab, ua rau nws heev soluble hauv dej. Cov duab txheej txheem schematic ntawm cov qauv molecular tau pom hauv daim duab 1. Lub purity ntawm tus qauv raw yog txog 88% raws li kev ntsuas ntawm HPLC. Rau qhov ua kom huv ntawm cov tebchaw ntawm cov polarity siab, cov qauv yuav tsis tshua tau khaws cia ntawm C18 txhua raws li peb cov kev paub dhau los. Yog li ntawd, C18AQ kem tau ua haujlwm rau cov qauv ua kom huv.
Daim duab 1. Lub schematic daim duab ntawm cov qauv cov qauv molecular.
Los npaj cov qauv tshuaj, 50 mg cov dej nyoos tau yaj nyob rau hauv 5 ml dej ntshiab thiab tom qab ntawd ultrasonicated thiaj li ua kom nws ua tiav kev daws teeb meem meej. Cov qauv kev daws teeb meem yog tom qab ntawd txhaj rau hauv flash rau los ntawm kev muaj kev tiv thaiv. Kev sim teeb tsa ntawm Flash Purification tau teev nyob hauv lub rooj 1.
| Lub cuab tam | Sepabean ™ tshuab 2 | |
| Tus saib xyuas | 12 g Sepaflash C18Aq RP Flash Carica, 20 - 45μm, 100 Å, tus lej xaj: Sw-5222- Sp (AQ) | |
| Lub paj | 204 nm, 220 nm | |
| Txawb theem | Hnyav A: Dej Cov kuab tshuaj B: Acetonitrile | |
| Txaus tus nqi | 15 ml / min | |
| Qauv Chaw thau khoom | 50 mg | |
| Grongent | Sijhawm (min) | Cov kuab tshuaj B (%) |
| 0 | 0 | |
| 19.0 | 8 | |
| 47.0 | 80 | |
| 52.0 | 80 | |
Cov txiaj ntsig thiab kev sib tham
Lub Flash Chromogram ntawm cov qauv ntawm C18AQ Cartridge tau qhia hauv daim duab 2. Raws li qhia hauv daim duab 2, cov qauv polar tau muab rau ntawm C18AQ Cartridge. Tom qab Labopholization rau cov feem ntau cov zauv feem, cov khoom lag luam muaj cov kev huv ntawm 96.2% (raws li qhia hauv daim duab 3) los ntawm HPLC Kev Ntsuam Xyuas. Cov txiaj ntsig tau taw qhia tias cov khoom siv huv tuaj yeem siv ntxiv hauv kauj ruam kauj ruam kev tshawb fawb tom ntej thiab kev loj hlob.
Daim duab 2. Lub Flash Chromoram ntawm cov qauv ntawm C18AQ Cartridge.
Daim duab 3. HPLC LeptomoGram ntawm cov khoom lag luam.
Hauv kev xaus, Sepaflash C18aq RP flash cartridge system sawb chinabography solabogyan tuaj yeem muab cov qauv kev ua kom huv thiab ua kom huv si.
Txog Sepaflash C18aq RP Flash Cartridges
Muaj cov koob c18aq RP flash cartridges nrog cov sib txawv los ntawm cov thev naus laus zis sib txawv (raws li qhia hauv Table 2).
| Yam khoom | Kem li | Txaus tus nqi (ml / min) | Max.pressure (psi / bar) |
| SW-5222-004-SP (AQ) | 5.4 g | 5-15 | 400 / 27.5 |
| SW-5222-012-AQ) | 20 g | 10-25 | 400 / 27.5 |
| SW-5222-025-SP (AQ) | 33 g | 10-25 | 400 / 27.5 |
| SW-5222-040-SP (AQ) | 48 g | 15-30 | 400 / 27.5 |
| SW-5222-080-SP (AQ) | 105 g | 25-50 | 350 / 24.0 |
| SW-522-120-SP (AQ) | 150 g | 30-60 | 300 / 20.7 |
| SW-5222-220-SP (AQ) | 300 g | 40-80 | 300 / 20.7 |
| SW-522-330-SP (AQ) | 420 g | 40-80 | 250 / 17.2 |
Rooj 2. Sepaflash C18aq RP Flash cartridges. Cov ntaub ntawv ntim: High-Efficiticquicical spherical c18 (aq) -bonded silica, 20 - 45 μ5 μ5 μm, 100 Å.
Yog xav paub ntxiv txog cov ncauj lus kom ntxaws ntawm Sepabean, lossis cov ntaub ntawv xaj ntawm Sepafash series nyem cartridges, thov mus saib peb lub vev xaib.
Lub sijhawm Post: Oct-26-2018