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Daim Ntawv Thov R & D Center
Lub hauv paus rau phau ntawv
Ion txauv cov chromatography (iec) yog cov qauv Chromatographic feem ntau siv los cais thiab ntxuav cov tebchaw uas tau nthuav tawm hauv Ionic daim ntawv hauv kev daws teeb meem. Raws li tus nqi sib txawv ntawm kev hloov pauv ntawm ions, IEC tuaj yeem faib ua ob hom, kev sib pauv cov chromatography thiab anion txauv cov chromatography. Hauv kev sib pauv hluav taws xob cov chromatography, cov pab pawg acidic tau sib raug zoo rau saum npoo ntawm kev sib cais xov xwm. Piv txwv li, sulfonic acid (-so3h) yog cov pab pawg siv sib luag hauv cov pawg sib xyaw ua ke (SCX) Hauv anion txauv cov chromatography, cov pab pawg alkaline tau sib raug zoo rau saum npoo ntawm kev sib cais xov xwm. Piv txwv, amerity amerity (-nr3oh, uas r mydrocarbon Group) feem ntau yog siv rau lwm pab pawg -n + R3 tuaj yeem ua rau muaj kev sib pauv muaj zog (SAx)
Ntawm cov khoom lag luam ntuj, flavonoids tau nyiam cov xim ntawm cov kws tshawb nrhiav vim tias lawv txoj haujlwm hauv kev tiv thaiv thiab kho cov kab mob plawv. Txij li thaum flavonoid molecules yog acidic vim tias muaj cov txheej txheem decoxyl, ion txauv cov roj ntsha tsis sib xws rau kev sib cais thiab huv ntawm cov khoom siv kua qaub. Hauv Flash chromatography, feem ntau siv sib cais media rau ion txauv yog silica gel Matrix qhov twg ion pab pawg tau sib raug zoo rau nws nto. Feem ntau siv cov qauv ion hauv flash chratography yog scx (feem ntau sulfonic acid pawg) thiab sax (feem ntau QUIdary Group). Hauv cov ntawv luam tawm yav dhau los nrog lub npe "daim ntawv thov ntawm cov kev ua kom tiav" los ntawm Santai thev naus laus zis, cov kab ntawv SCX tau ua haujlwm rau kev ua kom huv si ntawm alkaline cov tebchaw. Hauv cov ncej no, sib xyaw ntawm nruab nrab thiab acidic tus qauv tau siv los ua cov piv txwv los tshawb cov ntawv thov ntawm safification ntawm acidic teb.
Kev sim ntu
Daim duab 1. Lub schematic daim duab ntawm cov theem nyob ruaj khov rau saum npoo ntawm SAX sib cais xov xwm.
Hauv cov ncej no, ib kem SAX ua ntej-ntim nrog Quanet Qualine Herine Bonded silica tau siv (raws li qhia hauv daim duab 1). Kev sib xyaw ntawm cov chromone thiab 2,4-dihyrdoxybenzoic acid tau siv los ua cov qauv kom huv (raws li muaj nyob hauv daim duab 2). Cov sib tov tau yaj nyob hauv methanol thiab thauj mus rau lub nyem cartridge los ntawm ib tug injector. Kev sim teeb tsa ntawm Flash Purification yog teev nyob rau hauv lub rooj 1.
Daim duab 2. Cov qauv tshuaj ntawm ob qho kev sib xyaw hauv cov qauv sib tov.
| Lub cuab tam | Tshuab sepab ™ tshuab t | |||||
| Tus saib xyuas | 4 g Sepaflash Txheem Series Flash Carlica, 40 - 63 μm, 60 Å, tus lej xaj: S-5101-0004) | 4 g Sepaflash Bonded Series Sax Flash Carlica, 40 - 63 μm, 60 Å, Tus lej xaj: Sw-5004-IR) | ||||
| Lub paj | 254 nm (nrhiav kom tau), 280 nm (saib xyuas) | |||||
| Txawb theem | Kaus A: N-Hexane | |||||
| Cov Hnyav B: Ethyl Acetate | ||||||
| Txaus tus nqi | 30 ml / min | 20 ml / min | ||||
| Qauv Chaw thau khoom | 20 mg (kev sib xyaw ntawm cov khoom sib xyaw ua ke thiab ib feem b) | |||||
| Grongent | Lub sijhawm (CV) | Cov kuab tshuaj B (%) | Lub sijhawm (CV) | Cov kuab tshuaj B (%) | ||
| 0 | 0 | 0 | 0 | |||
| 1.7 | 12 | 14 | 100 | |||
| 3.7 | 12 | / | / | |||
| 16 | 100 | / | / | |||
| 18 | 100 | / | / | |||
Cov txiaj ntsig thiab kev sib tham
Firstly, cov qauv sib tov tau sib cais los ntawm theem theem nyem flash cartridge pre-packed nrog linica. Raws li qhia hauv Daim Duab 3, ob feem ntawm cov qauv raug kaw los ntawm Cartridge ib qho tom qab lwm tus. Tom ntej no, SAX flash cartridge tau siv rau kev ua kom huv ntawm cov qauv. Raws li qhia hauv Daim Duab 4, cov acidic tivthaiv B tau khaws tag nrho ntawm SAX cartridge. Lub nruab nrab tivthaiv ib qho tau maj mam muab los ntawm cov cartridge nrog kev tiv thaiv ntawm lub mobile theem.
Daim duab 3. Lub Flash Chromoram ntawm cov qauv ntawm ib ntu theem ib txwm cartridge.
Daim duab 4. Lub Flash Chromoram ntawm cov qauv ntawm SAX cartridge.
Sib piv rau daim duab 3 thiab Daim Duab 4, cov tivthaiv ib qho tsis sib xws ntawm cov duab sib txawv ntawm ob lub flash cartridges. Txhawm rau paub meej tias kev lees paub lub ncov yog sib xws nrog cov khoom siv, peb tuaj yeem siv tag nrho cov wavelength scanning feature uas tau ua rau hauv cov kev tswj software ntawm Sepabean ™ tshuab. Qhib cov txheej txheem sim ntawm ob qho sib cais, luag mus rau qhov sib xyaw ua ke ntawm ob lub ntsiab lus ntawm ob lub ntsiab lus hauv qab no axis (CV) Muab piv tag nrho wavelength cov ntaub ntawv ntawm ob cais, cov tivthaiv, cov tivthaiv ib qho muaj kev nqus tau cov khoom siv hauv ob sim. Vim li cas ntawm cov khoom lag luam muaj qhov tsis sib xws ntawm ob qho kev sib txawv ntawm cov kab mob uas muaj qhov sib txawv ntawm cov theem theem cartridge thiab sax cartridge. Yog li ntawd, kev hais lus qhuab qhia txawv rau cov sib xyaw ib thiab tus kab mob ntawm ob lub captistent, ua rau cov duab tsis sib xws ntawm cov chromatograms.
Daim duab 5. Lub wavelength tag nrho ntawm cov tivthaiv ib thiab lub impurity sib cais los ntawm theem theem ib txwm cartridge.
Daim duab 6. Lub wavelength tag nrho ntawm cov tivthaiv ib thiab kab mob sib cais los ntawm SAX cartridge.
Yog tias cov khoom lag luam tsis pub luam tawm yog qhov nruab nrab ntawm yog ib qho, ua haujlwm kom yooj yim tuaj yeem ua tiav los ntawm kev siv SAX kom tau txais kev lees paub tom qab chaw thau khoom. Ntawm qhov tod tes, yog tias cov khoom lag luam cov khoom lag luam yog sau rau ntawm theem kev sim siab, thaum cov qauv tau raug tshem tawm rau hauv Methanol daws teeb meem tau muaj 5% acetic acid. Cov acetate ions hauv lub xov tooj mobile yuav sib tw nrog cov theem, yog hais txog cov theem ntawm SAX cartridge, yog li hais txog cov cartridge kom tau txais cov khoom lag luam. Tus chromatogram ntawm cov qauv sib cais hauv ion kev sib pauv hom tau qhia hauv daim duab 7.
Daim duab 7. Lub Flash ChromoGram ntawm cov khoom siv b eleced hauv ion txauv hom ntawm sax cartridge.
Hauv kev xaus, acidic lossis nruab nrab cov qauv tuaj yeem ua kom sai ntawm SAX cartridge ua ke nrog theem theem cartridge siv cov kuab sib txawv. Tsis tas li ntawd, nrog kev pab ntawm cov nthwv wavelength scanning feature ua tau yooj yim thiab paub meej meej, pab cov kev sib cav sib ceg thiab yog li txhim kho kev ua haujlwm tau zoo.
| Yam khoom | Kem li | Txaus tus nqi (ml / min) | Max.pressure (psi / bar) |
| Sw-5001-004-ir | 5.9 g | 10-20 | 400 / 27.5 |
| Sw-5001-012-ir | 23 g | 15-30 | 400 / 27.5 |
| Sw-5001-025-ir | 38 g | 15-30 | 400 / 27.5 |
| Sw-5001-040-ir | 55 g | 00 | 400 / 27.5 |
| Sw-5001-080-ir | 122 g | 30-60 | 350 / 24.0 |
| Sw-5001-120-ir | 180 g | 40-80 | 300 / 20.7 |
| Sw-5001-220-ir | 340 G | 50-100 | 300 / 20.7 |
| Sw-5001-330-ir | 475 g | 50-100 | 250 / 17.2
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Rooj 2. Sepaflash Bonded Series SX Flash Cartridges. Cov ntaub ntawv ntim: ultra-ntshiab tsis xwm yeem-bonded silica, 40 - 63 μm, 60 Å.
Yog xav paub ntxiv txog cov ncauj lus kom ntxaws ntawm Sepabean ™Tshuab, lossis cov ntaub ntawv xaj ntawm Sepafash series nyem cartridges, thov mus saib peb lub vev xaib.
Lub Sijhawm Post: NOV-09-2018