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Peptide yog qhov sib txuas ntawm cov amino acids, txhua qhov uas muaj tshuaj lom neeg lub cev thiab cov tshuaj sib txawv vim yog cov amino acid residues confidues constituting nws cov kab ke. Nrog rau kev txhim kho theem tshuaj synthesis, cov tshuaj lom neeg hluavtaws ntawm ntau peptides tau ua tiav zoo. Txawm li cas los xij, vim txoj kev sib xyaw ua ke ntawm Peptide tau los ntawm cov khoom siv theem kawg, cov khoom kawg yuav tsum ua kom huv los ntawm kev sib cais sib cais. Cov cuab yeej ua kom huv si rau peptides suav nrog cov khoom siv chromatography (MW <1 KDA) Technologies, nyob rau Sepafash RP C18 cartridge tau siv rau kev ua kom huv si ntawm thymopentin (TP-5) thiab cov phiaj xwm cov khoom sib ntsib tau txais.
Daim duab 1. 20 Cov amino acids (luam tawm los ntawm www.bachem.com).
Muaj 20 hom amino acids uas muaj ntau yam nyob rau hauv cov lus qhia peptides. Cov amino acids tuaj yeem faib ua cov pab pawg hauv qab no raws li lawv cov tub ntxhais kawm ua ke thiab cov khoom siv hluav taws xob), polarged), acidged), raws li qhia hauv daim duab 1). Nyob rau hauv ib tug peptide sequence, yog tias cov amino acids sortituting cov txheej txheem polar yog feem ntau, sitamine, thiab lwm yam tuaj yeem muaj cov dej polarity. Thaum lub sij hawm cov txheej txheem lim tshuaj rau cov qauv polar peptide los ntawm theem Fydroographies, aq reversed theem checomatography txhua thiab lawv cov ntawv thov). Piv nrog cov kab c18 ib txwm muaj, cov kem txhim kho muaj feem ntau haum rau kev ua kom huv si polar lossis hydrophilic kuaj. Hauv cov ncej no, muaj zog polar peptide tau siv raws li cov qauv thiab ua kom muaj txiaj ntsig los ntawm C18Aq. Raws li qhov tshwm sim, cov khoom lag luam cov khoom sib tham txog cov cai tau txais thiab tuaj yeem siv rau hauv kev tshawb fawb hauv qab no thiab kev txhim kho.
| Lub cuab tam | Sepabean™Tshuab 2 | |||
| Tus saib xyuas | 12 g Sepaflash C18 RP Flash Carica, Spherical Silica, 20 - 45 μm, 100 Å, kev txiav txim nunber: sw-5222-012-sp) | 12 g Sepaflash C18aq RP Flash Carica, 20 - 45 μm, 100 Å, tus lej xaj: Sw-5222- Sp (AQ) | ||
| Lub paj | 254 nm, 220 nm | 214 nm | ||
| Txawb theem | Hnyav A: Dej Cov kuab tshuaj B: Acetonitrile | |||
| Txaus tus nqi | 15 ml / min | 20 ml / min | ||
| Qauv Chaw thau khoom | 30 mg | |||
| Grongent | Lub sijhawm (CV) | Cov kuab tshuaj B (%) | Sijhawm (min) | Cov kuab tshuaj B (%) |
| 0 | 0 | 0 | 4 | |
| 1.0 | 0 | 1.0 | 4 | |
| 10.0 | 6 | 7.5 | 18 | |
| 12.5 | 6 | 13.0 | 18 | |
| 16.5 | 10 | 14.0 | 22 | |
| 19.0 | 41 | 15.5 | 22 | |
| 21.0 | 41 | 18.0 | 38 | |
| / | / | 20.0 | 38 | |
| 22.0 | 87 | |||
| 29.0 | 87 | |||
Cov txiaj ntsig thiab kev sib tham
Txhawm rau muab cov qauv ua kom muaj zog rau cov qauv polar peptide ntawm cov kab c18 thiab C18AQ, peb siv cov flash huv si ntawm cov qauv thaum pib. Raws li muaj nyob hauv daim duab 2, vim muaj cov saw hlau dollapse ntawm C18 cov qauv tshwm sim los ntawm cov qauv ntawm C18 tsis tu ncua thiab raug kaw ncaj qha los ntawm cov theem mobile. Raws li qhov tshwm sim, cov qauv tsis tau sib cais thiab ua kom huv.
Daim duab 2. Lub Flash Chromoram ntawm cov qauv ntawm ib txwm C18 cartridge.
Tom ntej no, peb siv C18AQ rau lub eeb kom huv raws li tus qauv. Raws li muaj nyob hauv daim duab 3, tus peptide yog tau khaws cia rau ntawm kem thiab tom qab ntawd eluted tawm. Cov khoom lag luam tau sib cais los ntawm cov impurities hauv cov qauv nyoos thiab sau. Tom qab Lyophilization thiab tom qab ntawd tshuaj xyuas los ntawm HPLC, cov khoom siv huv si muaj cov kev coj dawb huv ntawm 98.2% thiab tuaj yeem siv ntxiv rau cov kauj ruam ntxiv rau cov kauj ruam ntxiv thiab kev txhim kho.
Daim duab 3. Lub Flash Chromoram ntawm cov qauv ntawm C18AQ Cartridge.
Hauv kev xaus, Sepaflash C18aq RP flash cartridge ua ke nrog lub flash chromatography zog Sepabean™Tshuab tuaj yeem muab cov tshuaj sai thiab ua tau zoo rau kev ua kom huv huv ntawm muaj zog polar lossis hydrophilic kuaj.
Muaj cov koob c18aq RP flash cartridges nrog cov sib txawv los ntawm cov thev naus laus zis sib txawv (raws li qhia hauv Table 2).
| Yam khoom | Kem li | Txaus tus nqi (ml / min) | Max.pressure (psi / bar) |
| SW-5222-004-SP (AQ) | 5.4 g | 5-15 | 400 / 27.5 |
| SW-5222-012-AQ) | 20 g | 10-25 | 400 / 27.5 |
| SW-5222-025-SP (AQ) | 33 g | 10-25 | 400 / 27.5 |
| SW-5222-040-SP (AQ) | 48 g | 15-30 | 400 / 27.5 |
| SW-5222-080-SP (AQ) | 105 g | 25-50 | 350 / 24.0 |
| SW-522-120-SP (AQ) | 150 g | 30-60 | 300 / 20.7 |
| SW-5222-220-SP (AQ) | 300 g | 40-80 | 300 / 20.7 |
| SW-522-330-SP (AQ) | 420 g | 40-80 | 250 / 17.2 |
Rooj 2. Sepaflash C18aq RP Flash cartridges. Cov ntaub ntawv ntim: High-Efficiticquicical spherical c18 (aq) -bonded silica, 20 - 45 μ5 μ5 μm, 100 Å.
Yog xav paub ntxiv txog cov ncauj lus kom ntxaws ntawm Sepabean, lossis cov ntaub ntawv xaj ntawm Sepafash series nyem cartridges, thov mus saib peb lub vev xaib.
Lub Sijhawm Post: Oct-12-2018