SepaFlash™ kem

Santai Solvent Lines Kit & Accessories

Tsis yog, kawg-capped silica yog insoluble nyob rau hauv tej yam uas nquag siv organic hnyav.

Txhawm rau kom zoo huv si nrog C18 flash kab, thov ua raws li cov kauj ruam no:
① Flush lub kem nrog 100% muaj zog (organic) hnyav rau 10 - 20 CVs (kem ntim), feem ntau methanol lossis acetonitrile.
② Flush lub kem nrog 50% muaj zog + 50% aqueous (yog tias xav tau ntxiv, suav nrog lawv) rau lwm 3 - 5 CVs.
③ Tshem tawm cov kab nrog qhov pib gradient rau 3 - 5 CVs.

Rau kab loj ntawm 4g thiab 330g, tus qauv Luer connector yog siv nyob rau hauv cov kab flash. Rau kem loj ntawm 800g, 1600g thiab 3000g, ntxiv connector adapters yuav tsum tau siv rau mount cov loj flash kab ntawm lub flash chromatography system. Thov xa mus rau cov ntaub ntawv Santai Adapter Kit rau 800g, 1600g, 3kg Flash Kab kom paub meej ntxiv.

Rau cov theem ib txwm muaj, nws raug nquahu kom siv lub sijhawm txawb uas qhov piv ntawm methanol tsis tshaj 25%.

Feem ntau, nws raug pom zoo kom siv lub sijhawm txawb qhov twg qhov piv ntawm cov kuab tshuaj polar tsis tshaj 5%. Cov kuab tshuaj polar muaj xws li DMSO, DMF, THF, TEA thiab lwm yam.

Khoom qauv thauj khoom yog cov txheej txheem muaj txiaj ntsig los thauj cov qauv kom huv mus rau hauv kem, tshwj xeeb tshaj yog rau cov qauv uas tsis tshua muaj solubility. Hauv qhov no, iLOK flash cartridge yog qhov kev xaiv tsim nyog heev.
Feem ntau, cov qauv yog yaj nyob rau hauv ib tug haum hnyav thiab adsorbed mus rau ib tug khoom adsorbant uas yuav zoo ib yam li siv nyob rau hauv flash kab, nrog rau diatomaceous earths los yog silica los yog lwm yam ntaub ntawv. Tom qab tshem tawm / evaporation ntawm cov residual kuab tshuaj, cov adsorbent yog muab tso rau saum ib feem ntawm kab los yog mus rau hauv ib tug khoob khoom loading cartridge. Yog xav paub ntxiv, thov mus saib cov ntaub ntawv iLOK-SL Cartridge Tus neeg siv phau ntawv qhia kom paub meej ntxiv.

Kem ntim yog kwv yees li qhov sib npaug ntawm qhov ntim tuag (VM) thaum tsis quav ntsej txog qhov ntim ntxiv hauv cov tubing txuas rau kem nrog lub injector thiab lub ntes.

Lub sij hawm tuag (tM) yog lub sij hawm xav tau rau elution ntawm cov khoom uas tsis tau khaws cia.

Tuag ntim (VM) yog qhov ntim ntawm lub xov tooj ntawm tes uas yuav tsum tau ua rau elution ntawm ib qho kev tivthaiv uas tsis tau khaws cia. Cov ntim tuag tuaj yeem suav los ntawm qhov sib npaug hauv qab no: VM = F0 * tM.

Ntawm cov kab zauv saum toj no, F0 yog tus nqi ntws ntawm theem mobile.

Tsis yog, kawg-capped silica yog insoluble nyob rau hauv tej yam uas nquag siv organic hnyav.

Cov silica flash kab yog pov tseg thiab siv ib zaug, tab sis nrog kev tuav kom zoo, cov silica cartridges tuaj yeem rov qab siv dua yam tsis muaj kev cuam tshuam kev ua haujlwm.
Txhawm rau rov siv dua, silica flash kem yuav tsum tau qhuav los ntawm huab cua compressed los yog yaug nrog thiab khaws cia hauv isopropanol.

Kev cia kom zoo yuav tso cai rau C18 flash kab rov qab siv:
• Tsis txhob cia cov kab kom qhuav tom qab siv.
• Tshem tawm tag nrho cov organic modifiers los ntawm yaug lub kem nrog 80% methanol lossis acetonitrile hauv dej rau 3 - 5 CVs.
• Khaws cov kem nyob rau hauv cov lus hais saum toj no cov dej ntws yaug nrog cov fittings kawg rau hauv qhov chaw.

Rau cov kab loj loj tshaj 220g, cov nyhuv thermal yog pom tseeb nyob rau hauv cov txheej txheem ntawm pre-equilibrium. Nws raug nquahu kom teeb tsa tus nqi ntws ntawm 50-60% ntawm cov lus pom zoo txaus hauv cov txheej txheem ua ntej sib npaug kom tsis txhob muaj kev cuam tshuam thermal.

Cov nyhuv thermal ntawm cov kuab tshuaj sib xyaw yog pom tseeb dua li cov kuab tshuaj ib leeg. Siv cov kuab tshuaj cyclohexane / ethyl acetate ua piv txwv, nws tau pom tias siv 100% cyclohexane hauv cov txheej txheem ua ntej sib npaug. Thaum kev sib npaug ua ntej ua tiav, qhov kev sib cais sib cais tuaj yeem ua tau raws li cov txheej txheem preset hnyav.