Tsarkake Cire Haraji ta Injin SepaBean™

Meiyuan Qian, Yuefeng Tan, Bo Xu
Cibiyar R&D Application

Gabatarwa
Taxus (Taxus chinensis ko Chinese yew) tsiro ne na daji da ƙasar ke kiyaye shi.Tsire-tsire ne da ba kasafai ba kuma mai hatsarin gaske wanda glaciers Quaternary ya bari.Har ila yau, ita ce kawai tsire-tsire masu magani a duniya.Ana rarraba harajin haraji a cikin yanki mai zafi na arewacin hemisphere zuwa tsakiyar tsakiyar yankin, tare da kusan nau'ikan 11 a duniya.Akwai nau'ikan guda 4 da kuma 1 iri-iri a cikin Arewa, wato Arbus na Arewa, Yunnan Hifus, Haraji, Tibetan Hipus da Kudancin Haɗa kai da Kudancin Haɗa kai.Ana rarraba wadannan nau'ikan guda biyar a kudu maso yammacin kasar Sin, Kudancin kasar Sin, Sin ta tsakiya, gabashin kasar Sin, arewa maso yammacin kasar Sin, arewa maso gabashin kasar Sin da Taiwan.Tsiren Taxus sun ƙunshi nau'ikan sinadarai iri-iri, waɗanda suka haɗa da haraji, flavonoids, lignans, steroids, phenolic acid, sesquiterpenes da glycosides.Shahararriyar maganin ciwon tumo Taxol (ko Paclitaxel) wani nau'i ne na haraji.Taxol yana da hanyoyin magance ciwon daji na musamman.Taxol na iya "daskare" microtubules ta hanyar haɗawa tare da su kuma ya hana microtubules daga raba chromosomes a lokacin rarraba tantanin halitta, don haka yana haifar da mutuwar sel masu rarraba, musamman masu saurin yaduwa da kwayoyin cutar kansa [1].Bugu da ƙari kuma, ta hanyar kunna macrophages, Taxol yana haifar da raguwa a cikin masu karɓa na TNF-α (tumor necrosis factor) da kuma sakin TNF-α, ta haka ne kashe ko hana ƙwayoyin tumor [2].Bugu da ƙari, Taxol na iya haifar da apoptosis ta hanyar yin aiki akan hanyar mai karɓa na apoptotic wanda Fas / FasL ya shiga tsakani ko kunna tsarin cysteine ​​protease [3].Saboda da mahara manufa anticancer sakamako, Taxol da ake amfani da ko'ina a cikin lura da ovarian ciwon daji, nono, non-small cell huhu ciwon daji (NSCLC), ciki ciwon daji, esophageal ciwon daji, mafitsara ciwon daji, prostate cancer, m melanoma, kai da wuyansa. kansa, da sauransu[4].Musamman ga ciwon nono mai ci gaba da ciwon daji na ovarian, Taxol yana da sakamako mai ban mamaki, saboda haka an san shi da "layin tsaro na ƙarshe don maganin ciwon daji".

Taxol shine maganin da ya fi shahara a kasuwannin duniya a shekarun baya-bayan nan kuma ana daukarsa a matsayin daya daga cikin magungunan da ake amfani da su wajen magance cutar kansa a cikin shekaru 20 masu zuwa.A cikin 'yan shekarun nan, tare da haɓakar haɓakar yawan jama'a da cutar kansa, buƙatar Taxol ita ma ta karu sosai.A halin yanzu, Taxol da ake buƙata don bincike na asibiti ko na kimiyya ana fitar da shi kai tsaye daga Taxus.Abin takaici, abun ciki na Taxol a cikin tsire-tsire ya ragu sosai.Misali, abun ciki na Taxol shine kawai 0.069% a cikin haushi na Taxus brevifolia, wanda galibi ana ɗauka yana da mafi girman abun ciki.Don hakar 1 g na Taxol yana buƙatar kimanin kilogiram 13.6 na haushin Taxus.Dangane da wannan kiyasin, ana ɗaukar bishiyar Taxus guda 3 – 12 waɗanda ke da shekaru sama da 100 don jinyar mai ciwon daji na kwai.A sakamakon haka, an sare itatuwan Taxus masu yawa, wanda ya haifar da kusan bacewa ga wannan nau'in nau'i mai daraja.Bugu da ƙari, Taxus yana da talauci sosai a cikin albarkatun kuma yana jinkirin girma, wanda ya sa ya zama mai wahala ga ci gaba da amfani da Taxol.

A halin yanzu, an kammala jimlar Taxol cikin nasara.Duk da haka, hanyarsa ta roba tana da sarƙaƙƙiya kuma mai tsada, wanda ya sa ba ta da mahimmancin masana'antu.Hanyar Semi-Synthetic ta Taxol yanzu ta cika balagagge kuma ana ɗaukarta a matsayin hanya mai inganci don faɗaɗa tushen Taxol baya ga shuka wucin gadi.A taƙaice, a cikin Semi-synthesis na Taxol, ana fitar da sinadarin Taxol precursor wanda yake da yawa a cikin tsire-tsire na Taxus sannan kuma a canza shi zuwa Taxol ta hanyar haɗin sinadarai.Abubuwan da ke cikin 10-deacetylbaccatin Ⅲ a cikin alluran Taxus baccata na iya zama har zuwa 0.1%.Kuma allura suna da sauƙin sake haɓakawa idan aka kwatanta da haushi.Saboda haka, Semi-kira ta Taxol dangane da 10-deacetylbaccatin Ⅲ yana jan hankali sosai daga masu bincike[5] (kamar yadda aka nuna a cikin Hoto 1).

Hoto 1. Hanyar Semi-synthetic Taxol bisa 10-deacetylbaccatin Ⅲ.

A cikin wannan post ɗin, cirewar shukar Taxus an tsarkake shi ta hanyar walƙiya na shirye-shiryen ruwa chromatography na'urar SepaBean ™ a hade tare da SepaFlash C18 reversed-phase (RP) filashin filashi wanda Santai Technologies ke samarwa.An samo samfurin da aka yi niyya don saduwa da buƙatun tsabta kuma ana iya amfani dashi a cikin binciken kimiyya na gaba, yana ba da mafita mai tsada don saurin tsarkakewa na irin waɗannan samfuran halitta.