Tsabtace Tsabtace Tsabtace Tsabtace a cikin Magungunan rigakafi ta Rukunin C18AQ

Mingzu Yang, Bo Xu
Cibiyar R&D Application

Gabatarwa
Magungunan rigakafi rukuni ne na metabolites na biyu da ƙananan ƙwayoyin cuta ke samarwa (ciki har da ƙwayoyin cuta, fungi, actinomycetes) ko mahaɗan makamantansu waɗanda aka haɗa su ta hanyar sinadarai ko sinadarai.Magungunan rigakafi na iya hana haɓakawa da rayuwan wasu ƙananan ƙwayoyin cuta.Kwayoyin cuta na farko da ɗan adam ya gano, penicillin, masanin ƙwayoyin cuta na Biritaniya Alexander Fleming ne ya gano shi a cikin 1928. Ya lura cewa ƙwayoyin cuta da ke kusa da mold ba za su iya girma a cikin tasa na al'ada na staphylococcus wanda ya gurɓata da mold.Ya bayyana cewa dole ne ya ɓoye wani abu mai kashe ƙwayoyin cuta, wanda ya sanya wa suna penicillin a cikin 1928. Duk da haka, ba a tsarkake abubuwan da ke aiki a lokacin ba.A shekara ta 1939, Ernst Chain da Howard Florey na Jami'ar Oxford sun yanke shawarar samar da wani maganin da zai iya magance cututtukan ƙwayoyin cuta.Bayan tuntuɓar Fleming don samun nau'ikan nau'ikan, sun sami nasarar cirewa da tsarkake penicillin daga nau'in.Don nasarar ci gaban su na penicillin a matsayin maganin warkewa, Fleming, Chain da Florey sun raba lambar yabo ta Nobel a cikin Magunguna ta 1945.

Ana amfani da maganin rigakafi azaman maganin kashe ƙwayoyin cuta don magance ko hana cututtukan ƙwayoyin cuta.Akwai manyan nau'ikan maganin rigakafi da yawa da aka yi amfani da su azaman magungunan kashe qwari: β-lactam maganin rigakafi (ciki har da penicillin, cephalosporin, da sauransu), maganin rigakafi na aminoglycoside, maganin rigakafi na macrolide, maganin rigakafi tetracycline, chloramphenicol (cikakken maganin rigakafi), da dai sauransu. Tushen maganin rigakafi sun haɗa da. nazarin halittu fermentation, Semi-kira da jimlar kira.Magungunan rigakafi da aka samar ta hanyar fermentation na halitta suna buƙatar gyara su ta hanyoyin sinadarai saboda kwanciyar hankali na sinadarai, illa masu guba, bakan ƙwayoyin cuta da sauran batutuwa.Bayan gyare-gyare ta hanyar sinadarai, maganin rigakafi na iya samun ƙarin kwanciyar hankali, rage tasirin sakamako masu guba, faɗaɗa nau'in ƙwayoyin cuta, rage juriya na ƙwayoyi, inganta yanayin rayuwa, kuma ta haka inganta tasirin magani.Saboda haka, Semi-Synthetic maganin rigakafi a halin yanzu shi ne mafi mashahuri shugabanci a cikin ci gaban da kwayoyin kwayoyi.

A cikin ci gaban Semi-Synthetic maganin rigakafi, maganin rigakafi da kaddarorin na low tsarki, kuri'a na by-kayayyakin da hadaddun aka gyara tun da aka samu daga microbial fermentation kayayyakin.A wannan yanayin, bincike da kula da ƙazanta a cikin maganin rigakafi na semi-synthetic yana da mahimmanci.Don gane da kuma kwatanta ƙazanta yadda ya kamata, ya zama dole don samun isasshen adadin ƙazanta daga samfurin roba na maganin rigakafi na semi-synthetic.Daga cikin fasahohin shirye-shiryen ƙazanta da aka saba amfani da su, chromatography mai walƙiya hanya ce mai tsada tare da fa'idodi kamar babban adadin lodin samfur, ƙarancin farashi, adana lokaci, da sauransu. Masu bincike na roba sun ƙara yin amfani da chromatography Flash.

A cikin wannan post ɗin, an yi amfani da babban ƙazanta na maganin rigakafi na aminoglycoside Semi-synthetic azaman samfuri kuma an tsarkake shi ta harsashi na SepaFlash C18AQ haɗe tare da tsarin chromatography na walƙiya SepaBean™ inji.An sami nasarar samun nasarar samfurin da aka yi niyya don saduwa da buƙatun, yana ba da shawarar ingantacciyar mafita don tsarkakewar waɗannan mahadi.