Aikace-aikacen CO18AQ a cikin tsarkakewa na karfi polar

Rui Huang, Bo Xu
Aikace-aikacen R & D Cirini

Shigowa da
Peptide wani fili ne ya ƙunshi amino acid, kowane ɗayan kayan aikin jiki na musamman saboda nau'ikan sunadarai saboda nau'ikan nau'ikan nau'ikan da aka tsara kuma da odar Amino acid ya tsara tsarinta. Tare da ci gaban mimin m synthesisis, da syntharis na sinadarai na peptides daban-daban peptides ya sami babban ci gaba. Koyaya, saboda rikice-rikice na pepticated na pepthide samu ta m yanayin kira, ya kamata a tsarkake samfurin ƙarshe da amintattun hanyoyin rabuwa. Hanyoyin tsarkakewa na yau da kullun don peptides sun haɗa da ion musanya cromatography (RP-HPLC), da sauran kuɗi na rabuwa da kayan maye, da sauransu), Santai Fasaha, wanda aka samo katakon katakon gyaran ruwa na tsattsagewa na thypentin (TP-5) da kuma samfurin da aka samo buƙatun da ake buƙata.

Hoto 1. 20 Amino acid (wanda aka kirkira daga www.bachem.com).

Akwai nau'ikan amino acid iri 20 da suka zama ruwan dare gama gari a cikin abubuwan da peptides. Wadannanoo acid za'a iya raba su zuwa wadannan kungiyoyi gwargwadon iko da acid da acid na acid: m ko na asali (kamar yadda aka nuna a hoto 1). A cikin jerin peptide, idan amino acid din da ke haifar da jerin abubuwa galibi sune (kamar yadda aka yiwa alama, da sauransu, da sauransu suna da ƙarfi a cikin ruwa. A lokacin da aka tsara tsarkakewar tsinkaye don wannan samfuran Pepar Peptide, Cancer Candrophic Candropes: Aq ta rushewar kayan aikin cromatography ginshiƙai da aikace-aikacen su). Idan aka kwatanta da ginshiƙan C18 na yau da kullun, ingantattun kayan ginshiƙai na C18AAQ sun fi dacewa da tsarkakewar polar ko samfuran Hydrophilic. A cikin wannan post, mai ƙarfi polar peptide anyi amfani dashi azaman samfurin da tsarkake ta C18AQ. A sakamakon haka, ana iya amfani da damar samfurin da ake buƙata kuma za'a iya amfani dashi a cikin bincike mai zuwa da ci gaba.