Aikace-aikacen ginshiƙan C18AQ A cikin Tsabtace Ƙarfin Ƙarfin Peptides

Rui Huang, Bo Xu
Cibiyar R&D Application

Gabatarwa
peptide wani fili ne da ya ƙunshi amino acid, kowannensu yana da sifofi na musamman na zahiri da sinadarai saboda nau'i da tsari na ragowar amino acid waɗanda suka haɗa da jerin sa.Tare da ci gaban m lokaci sunadarai kira, da sinadaran kira na daban-daban aiki peptides ya sami babban ci gaba.Koyaya, saboda rikitarwa mai rikitarwa na peptide da aka samu ta hanyar ingantaccen lokaci mai ƙarfi, samfuran ƙarshe yakamata a tsarkake su ta hanyoyin amintattun hanyoyin rabuwa.Hanyoyin tsarkakewa da aka saba amfani da su don peptides sun haɗa da ion musayar chromatography (IEC) da juyawa-lokaci high yi ruwa chromatography (RP-HPLC), wanda ke da disadvantages na low samfurin loading iya aiki, high kudin na rabuwa kafofin watsa labarai, rikitarwa da kuma tsada rabuwa kayan aiki, Da dai sauransu. Don saurin tsarkakewa na ƙananan ƙwayoyin ƙwayoyin peptides (MW <1 kDa), Santai Technologies an buga nasarar aikace-aikacen da aka yi nasara a baya, wanda aka yi amfani da harsashin SepaFlash RP C18 don saurin tsarkakewar thymopentin (TP-5) da Abubuwan da aka yi niyya don saduwa da buƙatun an samu.

Hoto 1. 20 na kowa amino acid (an sake fitowa daga www.bachem.com).

Akwai nau'ikan amino acid guda 20 waɗanda suka zama ruwan dare a cikin abubuwan peptides.Ana iya raba waɗannan amino acid zuwa ƙungiyoyi masu zuwa bisa ga polarity da dukiyar acid-base: wadanda ba polar (hydrophobic), iyakacin duniya (ba a caji), acidic ko asali (kamar yadda aka nuna a hoto 1).A cikin jeri na peptide, idan amino acid ɗin da suka ƙunshi jerin galibin polar ne (kamar yadda aka yi alama a cikin launin ruwan hoda a hoto na 1), kamar su Cysteine, Glutamine, Asparagine, Serine, Threonine, Tyrosine, da sauransu to wannan peptide na iya samun ƙarfi. polarity kuma zama sosai mai narkewa cikin ruwa.A lokacin aikin tsarkakewa na waɗannan samfuran peptide masu ƙarfi ta hanyar juzu'i na chromatography, wani abin al'ajabi da ake kira rushewar lokaci na hydrophobic zai faru (koma zuwa bayanin aikace-aikacen da aka buga a baya ta Santai Technologies: Rushewar Mataki na Hydrophobic, AQ Reversed Phase Chromatography ginshikan da Aikace-aikacensu).Idan aka kwatanta da ginshiƙan C18 na yau da kullum, ginshiƙan C18AQ da aka inganta sun fi dacewa don tsarkakewa na polar karfi ko samfurori na hydrophilic.A cikin wannan sakon, an yi amfani da peptide mai ƙarfi a matsayin samfurin kuma an tsarkake shi ta hanyar C18AQ.Sakamakon haka, an samo samfurin da aka yi niyya don saduwa da buƙatun kuma ana iya amfani da shi a cikin bincike da haɓaka mai zuwa.