FAQs

For SepaFlash^TM Standard Series columns, the connectors used are Luer-lock in and Luer-slip out. These columns could be directly mounted on ISCO’s CombiFlash systems.

For SepaFlash HP Series, Bonded Series or iLOKTM Series columns, the connectors used are Luer-lock in and Luer-lock out. These columns could also be mounted on ISCO’s CombiFlash systems via extra adaptors. For the details of these adaptors, please refer to the document Santai Adaptor Kit for 800g, 1600g, 3kg Flash Columns.

The parameter column volume (CV) is especially useful to determine scale-up factors. Some chemists think the internal volume of the cartridge (or column) without packing material inside is the column volume. However, the volume of an empty column is not the CV. The CV of any column or cartridge is the volume of the space not occupied by the material pre-packed in a column. This volume includes both the interstitial volume (the volume of the space outside the packed particles) and the particle’s own internal porosity (pore volume).

The alumina flash columns are an alternative option when the samples are sensitive and prone to degradation on silica gel.

The back pressure of flash column is related to the particle size of packed material. The packed material with smaller particle size will result in higher back pressure for the flash column. Therefore the flow rate of the mobile phase used in flash chromatography should be lowered accordingly in order to prevent the flash system from stop working.

The back pressure of flash column is also proportional to the length of column. Longer column body will result in higher back pressure for the flash column. Furthermore, the back pressure of flash column is inversely proportional to the ID (internal diameter) of the column body. Finally, the back pressure of flash column is proportional to the viscosity of the mobile phase used in flash chromatography.

Power on the instrument and wait for its prompt “Ready”. Make sure the iPad network connection is correct, and the router is powered on.

Check and confirm router status to make sure the iPad can be connected to the current router.

The equilibration is done when the column is totally wetted and looks translucent. Usually this can be done in flushing 2 ~ 3 CVs of the mobile phase. During the equilibration process, occasionally we might find that the column cannot be completely wetted. This is a normal phenomenon and will not compromise the separation performance.

Check if the tube rack is correctly placed in the right position. When this is done, the LCD screen on the tube rack should show a connected symbol.

If the tube rack is faulty, user can choose customized tube rack from the tube rack list in SePaBean App for temporary use . Or contact after-sale engineer.

Check whether the solvent bottle is lack of related solvent and replenish the solvent.

If the solvent line is full of solvent, please do not worry. Air bubble does not affect the flash separation since it is inevitable during solid sample loading. These bubbles will be gradually drained out during separation procedure.

Please open the back cover of the instrument, clean the pump piston rod with ethanol (analysis of pure or above), and rotate the piston while washing until the piston turns smoothly.

1. Instrument will not be able to pump the solvents when ambient temperature above 30℃, especially low boiling solvents, Such as dichloromethane or Ether.

Please ensure that the ambient temperature is below 30℃.

2. Air occupy the pipeline while the instrumnet out of operation for long time.

Please add ethanol to the ceramic rod of the pump head (analysis of pure or above) and increase the flow rate at the same time. The connector in front of the pump damaged or Loose, this will cause the line to leak air .Please check carefully whether the pipe connection is loose.

3. The connector in front of the pump damaged or Loose, it will cause the line to leak air .

Please confirm whether the pipe connector is in good condition.

The collect valve are blocked or aging. Please replace the three-way solenoid valve.

ADVICE: Please contact the after-sale engineer to deal with it.