SepaFlash™ HP Series

Short Description:

HP series flash columns are spin-welded and allow for higher pressure up to 400 psi, the available adapter facilitates offer compatibility with any flash system on the market.

※ Provide Luer-Lok in and Luer-Lok out flexibility for convenient column stacking.

※ Outstanding resolution with pre-packed high-efficiency silica gel (irregular, 25-40 µm, 60 Å; spherical, 20-45 µm, 70 Å).

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Product Detail

Application

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Product Introduction

HP series flash columns are spin-welded and allow for higher pressure of up to 400 psi. Available adapter facilitates compatibility with any flash system on the market. This series provides Luer-Lok in and Luer-Lok out flexibility for convenient column stacking. When pre-packed with high-efficiency silica gel (irregular, 25-40 μm, 60 Å; spherical, 20-45 μm, 70 Å), this series presents an outstanding resolution over conventional flash cartridges.

※ Solid, one-piece polypropylene body with thick walls for safety
※ Freely choose irregular silica or spherical silica according to your personal preference
※ Markedly improved resolution and higher sample loading capability
※ Spherical silica provides improved performance without increasing the system backpressure

Benefits of spherical silica gel

For spherical silica gel, strict quality controls from raw material to finished product ensures high lot-to-lot reproducibility and tightly controlled specifications.

※Consistency, reliability, reproducibility
※No contamination, lower backpressure
※Superior resolution
※Symmetrical peaks with no tailing
※Higher sample loading capacity

Product Parameters

UltraPure irregular silica, 40−63 µm, 60 Å (surface area 500 m² /g, pH 6.5–7.5, loading capacity 0.1–10%)

Item Number	Column Size	Sample Size(g)	Flow Rate(mL/min)	Cartridge Length (cm)	Cartridge ID (mm)	Max. Pressure(psi/bar)	Quantity per Box
Item Number	Column Size	Sample Size(g)	Flow Rate(mL/min)	Cartridge Length (cm)	Cartridge ID (mm)	Max. Pressure(psi/bar)	Small	Big
SW-5101-004	4 g	4 mg–0.4 g	15–40	113.8	12.4	400/27.5	24	120
SW-5101-012	12 g	12 mg–1.2 g	30–60	134.8	21.4	400/27.5	24	108
SW-5101-025	25 g	25 mg–2.5 g	30–60	184	21.4	400/27.5	18	72
SW-5101-040	40 g	40 mg–4.0 g	40–70	184.4	26.7	400/27.5	12	48
SW-5101-080	80 g	80 mg–8.0 g	50–100	257.4	31.2	350/24.0	10	20
SW-5101-120	120 g	120 mg–12 g	60–150	261.5	38.6	300/20.7	8	16
SW-5101-220	220 g	220 mg–22 g	80–220	223.5	61.4	300/20.7	4	8
SW-5101-330	330 g	330 mg–33 g	80–220	280.2	61.4	250/17.2	3	6

※ Compatible with all flash chromatography systems on the market.

High-efficiency irregular silica, 25−40 µm, 60 Å (surface area 500 m² /g, pH 6.5–7.5, loading capacity 0.1–15%)

Item Number	Column Size	Sample Size(g)	Flow Rate(mL/min)	Cartridge Length (cm)	Cartridge ID (mm)	Max. Pressure(psi/bar)	Quantity per Box
Item Number	Column Size	Sample Size(g)	Flow Rate(mL/min)	Cartridge Length (cm)	Cartridge ID (mm)	Max. Pressure(psi/bar)	Small	Big
SW-5102-004	4 g	4 mg–0.6 g	15–30	113.8	12.4	400/27.5	24	120
SW-5102-012	12 g	12 mg–1.8 g	25–50	134.8	21.4	400/27.5	24	108
SW-5102-025	25 g	25 mg–3.8 g	25–50	184	21.4	400/27.5	18	72
SW-5102-040	40 g	40 mg–6.0 g	30–60	184.4	26.7	400/27.5	12	48
SW-5102-080	80 g	80 mg–12 g	40–80	257.4	31.2	350/24.0	10	20
SW-5102-120	120 g	120 mg–18 g	45–90	261.5	38.6	300/20.7	8	16
SW-5102-220	220 g	220 mg–33 g	60–120	223.5	61.4	300/20.7	4	8
SW-5102-330	330 g	330 mg–50 g	60–120	280.2	61.4	250/17.2	3	6

※ Compatible with all flash chromatography systems on the market.

UltraPure spherical silica, 40−75 µm, 70 Å (surface area 500 m² /g, pH 6.0–8.0, loading capacity 0.1–10%)

Item Number	Column Size	Sample Size(g)	Flow Rate(mL/min)	Cartridge Length (cm)	Cartridge ID (mm)	Max. Pressure(psi/bar)	Quantity per Box
Item Number	Column Size	Sample Size(g)	Flow Rate(mL/min)	Cartridge Length (cm)	Cartridge ID (mm)	Max. Pressure(psi/bar)	Small	Big
SW-2101-004-SP	4 g	4 mg–0.4 g	15–40	113.8	12.4	400/27.5	24	120
SW-2101-012-SP	12 g	12 mg–1.2 g	30–60	134.8	21.4	400/27.5	24	108
SW-2101-025-SP	25 g	25 mg–2.5 g	30–60	184	21.4	400/27.5	18	72
SW-2101-040-SP	40 g	40 mg–4.0 g	40–70	184.4	26.7	400/27.5	12	48
SW-2101-080-SP	80 g	80 mg–8.0 g	50–100	257.4	31.2	350/24.0	10	20
SW-2101-120-SP	120 g	120 mg–12 g	60–150	261.5	38.6	300/20.7	8	16
SW-2101-220-SP	220 g	220 mg–22 g	80–220	223.5	61.4	300/20.7	4	8
SW-2101-330-SP	330 g	330 mg–33 g	80–220	280.2	61.4	250/17.2	3	6

※Compatible with all flash chromatography systems on the market.

High-efficiency spherical silica, 20−45 µm, 70 Å (surface area 500 m² /g, pH 6.0–8.0, loading capacity 0.1–15%)

Item Number	Column Size	Sample Size(g)	Flow Rate(mL/min)	Cartridge Length (cm)	Cartridge ID (mm)	Max. Pressure(psi/bar)	Quantity per Box
SW-2102-004-SP	4 g	4 mg–0.6 g	15–30	113.8	12.4	400/27.5	24
SW-2102-012-SP	12 g	12 mg–1.8 g	25–50	134.8	21.4	400/27.5	24
SW-2102-025-SP	25 g	25 mg–3.8 g	25–50	184	21.4	400/27.5	18
SW-2102-040-SP	40 g	40 mg–6.0 g	30–60	184.4	26.7	400/27.5	12
SW-2102-080-SP	80 g	80 mg–12 g	40–80	257.4	31.2	350/24.0	10
SW-2102-120-SP	120 g	120 mg–18 g	45–90	261.5	38.6	300/20.7	8
SW-2102-220-SP	220 g	220 mg–33 g	60–120	223.5	61.4	300/20.7	4
SW-2102-330-SP	330 g	330 mg–50 g	60–120	280.2	61.4	250/17.2	3

※Compatible with all flash chromatography systems on the market.

SepaFlash™ column stacking to improve resolution of normal phase flash chromatography

Purification of compounds that are difficult to separate by flash chromatography (ΔRf ≤ 0.2 between spots on TLC) often results in additional steps such as subsequent purification by preparative scale HPLC. It is possible to reduce the amount of additional work required for purification by simply stacking several prepacked SepaFlash™ columns end to end on a flash chromatography system.

In liquid chromatography, chemical species are separated on the basis of their differences in velocity as they move through the column. Increasing column length can significantly increase resolution. By stacking columns end to end the length to diameter (L to D) ratio is increased so that no major changes to the media and solvent system are necessary. Often this increased L to D is sufficient to provide successful separation of difficult mixtures due to close compounds retention time that is not obtained on a single column. The data illustrates the linear relationship between the resolution and overall column length.

HP Series - High-capacity Spherical Silica Gel

The Highest Purification Performance Available
HP series flash columns are precisely manufactured with high-capacity silica gel, and deliver the highest purification performance. The high-capacity spherical silica has 40% higher surface area, doubling the loading capacity of lower surface area silica.
※ High-capacity silica with 40% more surface area.
※ Higher loading means smaller, faster and cheaper cartridges can be used for any separation.
※ Less solvent required.
※ The highest peak resolution.

High-capacity spherical silica, 25 µm, 50 Å (surface area 700 m² /g, pH 5.0–8.0, loading capacity 0.1–30%)

Item Number	Column Size	Sample Size(g)	Flow Rate(mL/min)	Cartridge Length (cm)	Cartridge ID (mm)	Max. Pressure(psi/bar)	Quantity per Box
Item Number	Column Size	Sample Size(g)	Flow Rate(mL/min)	Cartridge Length (cm)	Cartridge ID (mm)	Max. Pressure(psi/bar)	Small	Big
SW-2102-004-SP(H)	4 g	4 mg–1.2 g	15–30	113.8	12.4	400/27.5	24	120
SW-2102-012-SP(H)	12 g	12 mg–3.6 g	25–50	134.8	21.4	400/27.5	24	108
SW-2102-025-SP(H)	25 g	25 mg–7.5 g	25–50	184	21.4	400/27.5	18	72
SW-2102-040-SP(H)	40 g	40 mg–12 g	30–60	184.4	26.7	400/27.5	12	48
SW-2102-080-SP(H)	80 g	80 mg–24 g	40–80	257.4	31.2	350/24.0	10	20
SW-2102-120-SP(H)	120 g	120 mg–36 g	45–90	261.5	38.6	300/20.7	8	16
SW-2102-220-SP(H)	220 g	220 mg–66 g	60–120	223.5	61.4	300/20.7	4	8
SW-2102-330-SP(H)	330 g	330 mg–99 g	60–120	280.2	61.4	250/17.2	3	6

※ Compatible with all flash chromatography systems on the market.

High-capacity spherical silica, 15 µm, 50 Å (NEW Product) (surface area 700 m² /g, pH 5.0–8.0, loading capacity 0.1–30%)

Item Number	Column Size	Sample Size(g)	Flow Rate(mL/min)	Cartridge Length (cm)	Cartridge ID (mm)	Max. Pressure(psi/bar)	Quantity per Box
Item Number	Column Size	Sample Size(g)	Flow Rate(mL/min)	Cartridge Length (cm)	Cartridge ID (mm)	Max. Pressure(psi/bar)	Small	Big
SW-2103-004-SP(H)	4 g	4 mg–1.2 g	10–15	113.8	12.4	400/27.5	24	120
SW-2103-012-SP(H)	12 g	12 mg–3.6 g	15–20	134.8	21.4	400/27.5	24	108
SW-2103-025-SP(H)	25 g	25 mg–7.5 g	15–20	184	21.4	400/27.5	18	72
SW-2103-040-SP(H)	40 g	40 mg–12 g	20–30	184.4	26.7	400/27.5	12	48
SW-2103-080-SP(H)	80 g	80 mg–24 g	30–40	257.4	31.2	350/24.0	10	20
SW-2103-120-SP(H)	120 g	120 mg–36 g	35–45	261.5	38.6	300/20.7	8	16
SW-2103-220-SP(H)	220 g	220 mg–66 g	50–65	223.5	61.4	300/20.7	4	8
SW-2103-330-SP(H)	330 g	330 mg–99 g	50–65	280.2	61.4	250/17.2	3	6

※ Compatible with all flash chromatography systems on the market.

Application of SepaFlash™ high-capacity spherical silica gel

Double Loading Capacity of SepaFlash™ Cartridges pre-packed with high-capacity silica gel (700 m² /g for 50 Å pore size) delivers the highest purification performance. The high-capacity 25 µm or 15 µm spherical silica has 40% higher surface area, doubling the loading capacity of lower surface area silica. Santai evaluated the performance of the SepaFlash™ columns compared to two well-known brands. Results show SepaFlash™ outperforms the competitors.

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